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- Characterization of Newly Isolated Bacteriophages Targeting Carbapenem-Resistant Klebsiella pneumoniae
- Bokyung Kim, Shukho Kim, Yoon-Jung Choi, Minsang Shin, Jungmin Kim
- J. Microbiol. 2024;62(12):1133-1153. Published online December 10, 2024
- DOI: https://doi.org/10.1007/s12275-024-00180-7
- 392 View
- 9 Download
- 1 Web of Science
- 2 Crossref
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Abstract
PDF - Klebsiella pneumoniae, a Gram-negative opportunistic pathogen, is increasingly resistant to carbapenems in clinical settings. This growing problem necessitates the development of alternative antibiotics, with phage therapy being one promising option. In this study, we investigated novel phages targeting carbapenem-resistant Klebsiella pneumoniae (CRKP) and evaluated their lytic capacity against clinical isolates of CRKP. First, 23 CRKP clinical isolates were characterized using Multi-Locus Sequence Typing (MLST), carbapenemase test, string test, and capsule typing. MLST classified the 23 K. pneumoniae isolates into 10 sequence types (STs), with the capsule types divided into nine known and one unknown type. From sewage samples collected from a tertiary hospital, 38 phages were isolated. Phenotypic and genotypic characterization of these phages was performed using Random Amplification of Polymorphic DNA-PCR (RAPD-PCR), transmission electron microscopy (TEM), and whole genome sequencing (WGS) analysis. Host spectrum analysis revealed that each phage selectively lysed strains sharing the same STs as their hosts, indicating ST-specific activity. These phages were subtyped based on their host spectrum and RAPD-PCR, identifying nine and five groups, respectively. Fourteen phages were selected for further analysis using TEM and WGS, revealing 13 Myoviruses and one Podovirus. Genomic analysis grouped the phages into three clusters: one closely related to Alcyoneusvirus, one to Autographiviridae, and others to Straboviridae. Our results showed that the host spectrum of K. pneumoniae-specific phages corresponds to the STs of the host strain. These 14 novel phages also hold promise as valuable resources for phage therapy against CRKP.
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Citations
Citations to this article as recorded by
- Evaluation of Bacteriophage and Antibiotic Synergy Against Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates
Bokyung Kim, Shukho Kim, Yoon-Jung Choi, Minsang Shin, Jungmin Kim
Journal of Bacteriology and Virology.2025; 55(2): 131. CrossRef - Possible regulatory network and associated pathways governing the expression of ADH2 in Saccharomyces cerevisiae
Pratima Sarkar, Rohan Nath, Prity Adhikary, Arindam Bhattacharjee
Current Genetics.2025;[Epub] CrossRef
- Evaluation of Bacteriophage and Antibiotic Synergy Against Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates
- NEDD4 Regulated Pyroptosis Occurred from Co‑infection between Influenza A Virus and Streptococcus pneumoniae
- Jiangzhou You , Linlin Zhou , Xudong San , Hailing Li , Mingyuan Li , Baoning Wang
- J. Microbiol. 2023;61(8):777-789. Published online October 4, 2023
- DOI: https://doi.org/10.1007/s12275-023-00076-y
- 357 View
- 0 Download
- 9 Web of Science
- 8 Crossref
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Abstract
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- Co-infection of respiratory tract viruses and bacteria often result in excess mortality, especially pneumonia caused by influenza viruses and Streptococcus pneumoniae. However, the synergistic mechanisms remain poorly understood. Therefore, it is necessary to develop a clearer understanding of the molecular basis of the interaction between influenza virus and Streptococcus pneumonia. Here, we developed the BALB/c mouse model and the A549 cell model to investigate inflammation and pyroptotic cell death during co-infection. Co-infection significantly activated the NLRP3 inflammasome and induced pyroptotic cell death, correlated with excess mortality. The E3 ubiquitin ligase NEDD4 interacted with both NLRP3 and GSDMD, the executor of pyroptosis. NEDD4 negatively regulated NLRP3 while positively regulating GSDMD, thereby modulating inflammation and pyroptotic cell death. Our findings suggest that NEDD4 may play a crucial role in regulating the GSDMD-mediated pyroptosis signaling pathway. Targeting NEDD4 represents a promising approach to mitigate excess mortality during influenza pandemics by suppressing synergistic inflammation during co-infection of influenza A virus and Streptococcus pneumoniae.
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Citations
Citations to this article as recorded by- GSDMD and GSDME exhibit distinct roles in enteric coronavirus PDCoV-induced pyroptosis and inflammatory responses
Chenyu Li, Yuting Shi, Chunying Xie, Kaiqi Duan, Tong Ding, Xiangfei Xu, Liurong Fang, Yanrong Zhou, Shaobo Xiao, Tom Gallagher
Journal of Virology.2025;[Epub] CrossRef - Updated insights into the molecular networks for NLRP3 inflammasome activation
Seungwha Paik, Jin Kyung Kim, Hyo Jung Shin, Eun-Jin Park, In Soo Kim, Eun-Kyeong Jo
Cellular & Molecular Immunology.2025; 22(6): 563. CrossRef - Universal and highly sensitive detection of influenza A virus and streptococcus pneumoniae using WGA-modified magnetic SERS nanotags-based lateral flow assay
Xiaofei Jia, Zhenzhen Liu, Juan Zhou, Chunran Cao, Yunwei Hao, Jin Chen, Han Han, Jing Liang, Zhibin Zhao, Yi Wang, Zhendong Niu, Rui Xiao
Nanomedicine: Nanotechnology, Biology and Medicine.2025; 69: 102853. CrossRef - Post-influenza bacterial infection: mechanisms of pathogenesis and advances in therapeutic strategies
Biao Lei, Shun Wang, Linzhong Yu, Qinhai Ma
Frontiers in Microbiology.2025;[Epub] CrossRef - Pyroptosis in Respiratory Virus Infections: A Narrative Review of Mechanisms, Pathophysiology, and Potential Therapeutic Interventions
Runqi Lin, Barbara N. Porto
Microorganisms.2025; 13(9): 2109. CrossRef - Yinqin Qingfei granules alleviate Mycoplasma pneumoniae pneumonia via inhibiting NLRP3 inflammasome-mediated macrophage pyroptosis
Zhe Song, Chengen Han, Guangzhi Luo, Guangyuan Jia, Xiao Wang, Baoqing Zhang
Frontiers in Pharmacology.2024;[Epub] CrossRef - Overexpression of DTX1 inhibits D-GalN/TNF-α-induced pyroptosis and inflammation in hepatocytes by regulating NLRP3 ubiquitination
Mingshui Liu, Jing Gu, Li Chen, Wei Sun, Xiaoping Huang, Jianhe Gan
Toxicology Research.2024;[Epub] CrossRef - NLRP3 Inflammasomes: Dual Function in Infectious Diseases
Yanbo Li, Rui Qiang, Zhengmin Cao, Qingjuan Wu, Jiuchong Wang, Wenliang Lyu
The Journal of Immunology.2024; 213(4): 407. CrossRef
- GSDMD and GSDME exhibit distinct roles in enteric coronavirus PDCoV-induced pyroptosis and inflammatory responses
- Varicella‑Zoster Virus ORF39 Transmembrane Protein Suppresses Interferon‑Beta Promoter Activation by Interacting with STING
- Gwang Myeong Lee , Shuang Gong , Seong&# , Hyemin Ko , Woo&# , Jihyun Lee , Ok Sarah Shin , Jin&#
- J. Microbiol. 2023;61(2):259-270. Published online February 20, 2023
- DOI: https://doi.org/10.1007/s12275-023-00019-7
- 292 View
- 1 Download
- 1 Web of Science
- 1 Crossref
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Abstract
PDF
- Varicella-Zoster virus (VZV) causes varicella in primary infection of children and zoster during reactivation in adults. Type I interferon (IFN) signaling suppresses VZV growth, and stimulator of interferon genes (STING) plays an important role in anti-VZV responses by regulating type I IFN signaling. VZV-encoded proteins are shown to inhibit STING-mediated activation of the IFN-β promoter. However, the mechanisms by which VZV regulates STING-mediated signaling pathways are largely unknown. In this study, we demonstrate that the transmembrane protein encoded by VZV open reading frame (ORF) 39 suppresses STING-mediated IFN-β production by interacting with STING. In IFN-β promoter reporter assays, ORF39 protein (ORF39p) inhibited STING-mediated activation of the IFN-β promoter. ORF39p interacted with STING in co-transfection assays, and this interaction was comparable to that of STING dimerization. The cytoplasmic N-terminal 73 amino acids region of ORF39P was not necessary for ORF39 binding and suppression of STING-mediated IFN-β activation. ORF39p also formed a complex containing both STING and TBK1. A recombinant VZV expressing HA-tagged ORF39 was produced using bacmid mutagenesis and showed similar growth to its parent virus. During HA-ORF39 virus infection, the expression level of STING was markedly reduced, and HA-ORF39 interacted with STING. Moreover, HA-ORF39 also colocalized with glycoprotein K (encoded by ORF5) and STING at the Golgi during virus infection. Our results demonstrate that the transmembrane protein ORF39p of VZV plays a role in evading the type I IFN responses by suppressing STINGmediated activation of the IFN-β promoter.
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Citations
Citations to this article as recorded by- Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro
Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi
Journal of Microbiology.2025; 63(5): e2501029. CrossRef
- Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro
- Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
- Syahriel Abdullah , Yoon Sin Oh , Min-Kyu Kwak , KhimPhin Chong
- J. Microbiol. 2021;59(2):164-174. Published online December 23, 2020
- DOI: https://doi.org/10.1007/s12275-021-0551-8
- 357 View
- 0 Download
- 11 Web of Science
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Abstract
PDF
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There have been relatively few studies which support a link
between Ganoderma boninense, a phytopathogenic fungus
that is particularly cytotoxic and pathogenic to plant tissues
and roots, and antimicrobial compounds. We previously observed
that liquid-liquid extraction (LLE) using chloroformmethanol-
water at a ratio (1:1:1) was superior at detecting
antibacterial activities and significant quantities of antibacterial
compounds. Herein, we demonstrate that antibacterial
secondary metabolites are produced from G. boninense mycelia.
Antibacterial compounds were monitored in concurrent
biochemical and biophysical experiments. The combined
methods
included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites. -
Citations
Citations to this article as recorded by- Techno‐Functional Biochemical Analysis and Food Applications of Edible Mushroom Powder
Subhra De, Prince Chawla, Sanju Bala Dhull, Gulden Goksen, Anarase Dattatray Arjun, Aarti Bains, Nadica Maltar Strmečki
Journal of Food Biochemistry.2025;[Epub] CrossRef -
Optimization of
Ganoderma lingzhi
triterpene extraction method and its hypoglycemic activity
Shuang Hua, Yanshuang Li, Feng Jin, Meiyao Gan, Xianshun Jiang, Ying Zhang, Bo Zhang, Xiao Li
Preparative Biochemistry & Biotechnology.2025; 55(9): 1180. CrossRef - Exploring the health benefits of Ganoderma: antimicrobial properties and mechanisms of action
Samantha C. Karunarathna, Nimesha M. Patabendige, Kalani K. Hapuarachchi, Itthayakorn Promputtha
Frontiers in Cellular and Infection Microbiology.2025;[Epub] CrossRef - Extraction, Isolation and Characterization of Bioactive Compounds from Euphorbia neriifolia (L.) Leaf and Evaluation of their Antioxidant Activity
Priya Chaudhary, Devendra Singh, Mukesh Meena, Pracheta Janmeda
Current Analytical Chemistry.2025; 21(5): 559. CrossRef - Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches
Trang Thi Thu Nguyen, Trinh Thi Tuyet Nguyen, Hoang Duc Nguyen, Tan Khanh Nguyen, Phu Tran Vinh Pham, Linh Thuy Thi Tran, Hong Khuyen Thi Pham, Phu Chi Hieu Truong, Linh Thuoc Tran, Manh Hung Tran
Heliyon.2024; 10(7): e28118. CrossRef - Medium composition optimization and characterization of polysaccharides extracted from Ganoderma boninense along with antioxidant activity
Qian-Zhu Li, Chuan Xiong, Wei Chee Wong, Li-Wei Zhou
International Journal of Biological Macromolecules.2024; 260: 129528. CrossRef - Plant Defense Inducers and Antioxidant Metabolites Produced During Oil Palm-Ganoderma boninense Interaction In Vitro
Neda Shokrollahi, Chai-Ling Ho, Nur Ain Izzati Mohd Zainudin, Mohd As’wad Bin Abdul Wahab, Mui-Yun Wong
Chemistry Africa.2023; 6(1): 499. CrossRef - Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques
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Yow-San Chan, Khim-Phin Chong
Molecules.2022; 27(3): 838. CrossRef - Review Update on the Life Cycle, Plant–Microbe Interaction, Genomics, Detection and Control Strategies of the Oil Palm Pathogen Ganoderma boninense
Izwan Bharudin, Anis Farhan Fatimi Ab Wahab, Muhammad Asyraff Abd Samad, Ng Xin Yie, Madihah Ahmad Zairun, Farah Diba Abu Bakar, Abdul Munir Abdul Murad
Biology.2022; 11(2): 251. CrossRef - Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria
Clément Huguet, Mélanie Bourjot, Jean-Michel Bellanger, Gilles Prévost, Aurélie Urbain
Applied Sciences.2022; 12(10): 5229. CrossRef
- Techno‐Functional Biochemical Analysis and Food Applications of Edible Mushroom Powder
Review
- Recent advances in the development of β-lactamase inhibitors
- Shivakumar S. Jalde , Hyun Kyung Choi
- J. Microbiol. 2020;58(8):633-647. Published online July 27, 2020
- DOI: https://doi.org/10.1007/s12275-020-0285-z
- 336 View
- 1 Download
- 23 Web of Science
- 22 Crossref
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Abstract
PDF
- β-Lactam antibiotics are the most commonly prescribed antibiotics worldwide; however, antimicrobial resistance (AMR) is a global challenge. The β-lactam resistance in Gram-negative bacteria is due to the production of β-lactamases, including extended-spectrum β-lactamases, metallo-β-lactamases, and carbapenem-hydrolyzing class D β-lactamases. To restore the efficacy of BLAs, the most successful strategy is to use them in combination with β-lactamase inhibitors (BLI). Here we review the medically relevant β-lactamase families and penicillins, diazabicyclooctanes, boronic acids, and novel chemical scaffold-based BLIs, in particular approved and under clinical development.
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Citations
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Yoshiki Kato, Toshio Yamaguchi, Haruka Nakagawa-Kamura, Yoshikazu Ishii, Akiko Shimizu-Ibuka, Pablo Power
Microbiology Spectrum.2024;[Epub] CrossRef - Current Strategy for Targeting Metallo-β-Lactamase with Metal-Ion-Binding Inhibitors
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Hao Chang, Renzhong Qiao, Chao Li
Chinese Chemical Letters.2024; : 110675. CrossRef - Exploring the dynamics of gut microbiota, antibiotic resistance, and chemotherapy impact in acute leukemia patients: A comprehensive metagenomic analysis
Ying Luo, Taha Majid Mahmood Sheikh, Xin Li, YuMeng Yuan, Fen Yao, Meimei Wang, Xiaoling Guo, Jilong Wu, Muhammad Shafiq, Qingdong Xie, Xiaoyang Jiao
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Fungal Genetics and Biology.2024; 172: 103889. CrossRef - Revisiting the Checkerboard to Inform Development of β-Lactam/β-Lactamase Inhibitor Combinations
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Antibiotics.2024; 13(4): 337. CrossRef - Role of β-Lactamase Inhibitors as Potentiators in Antimicrobial Chemotherapy Targeting Gram-Negative Bacteria
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Antibiotics.2024; 13(3): 260. CrossRef - The C5α-Methyl-Substituted Carbapenem NA-1-157 Exhibits Potent Activity against Klebsiella spp. Isolates Producing OXA-48-Type Carbapenemases
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ACS Infectious Diseases.2023; 9(5): 1123. CrossRef - Phenotypes, genotypes and breakpoints: an assessment of β-lactam/β-lactamase inhibitor combinations against OXA-48
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Foodborne Pathogens and Disease.2023; 20(7): 270. CrossRef - Sequential C−H Methylation Catalyzed by the B12‐Dependent SAM Enzyme TokK: Comprehensive Theoretical Study of Selectivities
Wen‐Hao Deng, Rong‐Zhen Liao
Chemistry – A European Journal.2023;[Epub] CrossRef - CMOS Spectrophotometric Microsystem for Malaria Detection
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- Functional and structural analyses of IMP-27 metallo-β-lactamase: evolution of IMP-type enzymes to overcome Zn(II) deprivation
Journal Article
- Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling
- Ling Wang , Xuemei Zhang , Guangying Wu , Yuhong Qi , Jinghui Zhang , Jing Yang , Hong Wang , Wenchun Xu
- J. Microbiol. 2020;58(4):330-339. Published online February 27, 2020
- DOI: https://doi.org/10.1007/s12275-020-9538-0
- 337 View
- 0 Download
- 11 Web of Science
- 10 Crossref
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Abstract
PDF
- Streptococcus pneumoniae is a Gram-positive pathogen with high morbidity and mortality globally but some of its pathogenesis remains unknown. Previous research has provided evidence that aminopeptidase N (PepN) is most likely a virulence factor of S. pneumoniae. However, its role in S. pneumoniae virulence and its interaction with the host remains to be confirmed. We generated a pepN gene deficient mutant strain and found that its virulence for mice was significantly attenuated as were in vitro adhesion and invasion of host cells. The PepN protein could induce a strong innate immune response in vivo and in vitro and induced secretion of IL-6 and TNF-α by primary peritoneal macrophages via the rapid phosphorylation of MAPK and PI3K/AKT signaling pathways and this was confirmed using specific pathway inhibitors. In conclusion, PepN is a novel virulence factor that is essential for the virulence of S. pneumoniae and induces host innate immunity via MAPK and PI3K/AKT signaling.
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Review
- REVIEW] Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea
- Kwan Soo Ko
- J. Microbiol. 2019;57(3):195-202. Published online October 2, 2018
- DOI: https://doi.org/10.1007/s12275-019-8491-2
- 350 View
- 0 Download
- 7 Web of Science
- 9 Crossref
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Abstract
PDF
- Antibiotic resistance is a global concern in public health. Antibiotic-resistant clones can spread nationally, internationally, and globally. This review considers representative antibiotic-resistant Gram-negative bacterial clones–CTX-M- 15-producing ST131 in Escherichia coli, extended-spectrum β-lactamase-producing ST11 and KPC-producing ST258 in Klebsiella pneumoniae, IMP-6-producing, carbapenem-resistant ST235 in Pseudomonas aeruginosa, and OXA-23- producing global clone 2 in Acinetobacter baumannii–that have disseminated worldwide, including in Korea. The findings highlight the urgency for systematic monitoring and international cooperation to suppress the emergence and propagation of antibiotic resistance.
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Citations
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Journal Articles
- Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
- Min Yong Jwa , Soyoung Jeong , Eun Byeol Ko , A Reum Kim , Hyun Young Kim , Sun Kyung Kim , Ho Seong Seo , Cheol-Heui Yun , Seung Hyun Han
- J. Microbiol. 2018;56(8):579-585. Published online July 25, 2018
- DOI: https://doi.org/10.1007/s12275-018-8347-1
- 381 View
- 0 Download
- 15 Crossref
-
Abstract
PDF
-
Streptococcus pneumoniae is a major respiratory pathogen
that causes millions of deaths worldwide. Although subunit
vaccines formulated with the capsular polysaccharides or
their protein conjugates are currently-available, low-cost
vaccines with wide serotype coverage still remain to be developed,
especially for developing countries. Recently, gamma-
irradiation has been considered as an effective inactivation
method
to prepare S. pneumoniae vaccine candidate. In this study, we investigated the immunogenicity and protective immunity of gamma-irradiated S. pneumoniae (r-SP), by comparing with heat-inactivated S. pneumoniae (h-SP) and formalin-inactivated S. pneumoniae (f-SP), both of which were made by traditional inactivation methods. Intranasal immunization of C57BL/6 mice with r-SP in combination with cholera toxin as an adjuvant enhanced S. pneumoniaespecific antibodies on the airway mucosal surface and in sera more potently than that with h-SP or f-SP under the same conditions. In addition, sera from mice immunized with r- SP potently induced opsonophagocytic killing activity more effectively than those of h-SP or f-SP, implying that r-SP could induce protective antibodies. Above all, immunization with r-SP effectively protected mice against S. pneumoniae infection. Collectively, these results suggest that gamma- irradiation is an effective method for the development of a killed whole cell pneumococcal vaccine that elicits robust mucosal and systemic immune responses. -
Citations
Citations to this article as recorded by- Acute otitis media pneumococcal disease burden and nasopharyngeal colonization in children due to serotypes included and not included in current and new pneumococcal conjugate vaccines
Michael Pichichero, Richard Malley, Ravinder Kaur, Robert Zagursky, Porter Anderson
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Yuyu Wang, Jianjiang Huang, Fang Zhang, Keli Shen, Bin Qiu
Growth Factors.2023; 41(4): 210. CrossRef - Occurrence of influenza and bacterial infections in cancer patients receiving radiotherapy in Ghana
Augustina K. Arjarquah, Evangeline Obodai, Hannah Ayettey Anie, Michael Aning Osei, John Kofi Odoom, Joseph H. K. Bonney, Eric Behene, Erasmus N. Kotey, James Aboagye, Stephen O. Nyarko, Jeannette Bentum, Clara Yeboah, Selassie Kumordjie, Bright Agbodzi,
PLOS ONE.2022; 17(7): e0271877. CrossRef - Low-Energy Electron Irradiation of Tick-Borne Encephalitis Virus Provides a Protective Inactivated Vaccine
Julia Finkensieper, Leila Issmail, Jasmin Fertey, Alexandra Rockstroh, Simone Schopf, Bastian Standfest, Martin Thoma, Thomas Grunwald, Sebastian Ulbert
Frontiers in Immunology.2022;[Epub] CrossRef - Non-capsular based immunization approaches to prevent Streptococcus pneumoniae infection
Pedro H. Silva, Yaneisi Vázquez, Camilo Campusano, Angello Retamal-Díaz, Margarita K. Lay, Christian A. Muñoz, Pablo A. González, Alexis M. Kalergis, Susan M. Bueno
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - A Nonadjuvanted Whole-Inactivated Pneumococcal Vaccine Induces Multiserotype Opsonophagocytic Responses Mediated by Noncapsule-Specific Antibodies
Shannon C. David, Erin B. Brazel, Eve V. Singleton, Vikrant Minhas, Zoe Laan, Catherine Scougall, Austen Y. Chen, Hui Wang, Chloe J. Gates, Kimberley T. McLean, Jeremy S. Brown, Giuseppe Ercoli, Rachel A. Higgins, Paul V. Licciardi, Kim Mulholland, Justin
mBio.2022;[Epub] CrossRef - Developing green and sustainable concrete in integrating with different urban wastes
Huaguo Chen, Cheuk Lun Chow, Denvid Lau
Journal of Cleaner Production.2022; 368: 133057. CrossRef - Intranasal Vaccine Delivery Technology for Respiratory Tract Disease Application with a Special Emphasis on Pneumococcal Disease
William Walkowski, Justin Bassett, Manmeet Bhalla, Blaine A. Pfeifer, Elsa N. Bou Ghanem
Vaccines.2021; 9(6): 589. CrossRef - Immune Responses to Irradiated Pneumococcal Whole Cell Vaccine
Eunbyeol Ko, Soyoung Jeong, Min Yong Jwa, A Reum Kim, Ye-Eun Ha, Sun Kyung Kim, Sungho Jeong, Ki Bum Ahn, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Vaccines.2021; 9(4): 405. CrossRef - Controlling the Colonization of Clostridium perfringens in Broiler Chickens by an Electron-Beam-Killed Vaccine
Palmy R. Jesudhasan, Sohini S. Bhatia, Kirthiram K. Sivakumar, Chandni Praveen, Kenneth J. Genovese, Haiqi L. He, Robert Droleskey, Jack L. McReynolds, James A. Byrd, Christina L. Swaggerty, Michael H. Kogut, David J. Nisbet, Suresh D. Pillai
Animals.2021; 11(3): 671. CrossRef - Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities
Jasmin Fertey, Martin Thoma, Jana Beckmann, Lea Bayer, Julia Finkensieper, Susann Reißhauer, Beatrice Sarah Berneck, Leila Issmail, Jessy Schönfelder, Javier Portillo Casado, Andre Poremba, Frank-Holm Rögner, Bastian Standfest, Gustavo R. Makert, Lia Walc
Scientific Reports.2020;[Epub] CrossRef - Innate and Adaptive Immune Responses against Bordetella pertussis and Pseudomonas aeruginosa in a Murine Model of Mucosal Vaccination against Respiratory Infection
Catherine B. Blackwood, Emel Sen-Kilic, Dylan T. Boehm, Jesse M. Hall, Melinda E. Varney, Ting Y. Wong, Shelby D. Bradford, Justin R. Bevere, William T. Witt, F. Heath Damron, Mariette Barbier
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Jasmin Fertey, Lea Bayer, Sophie Kähl, Rukiya M. Haji, Anke Burger-Kentischer, Martin Thoma, Bastian Standfest, Jessy Schönfelder, Javier Portillo Casado, Frank-Holm Rögner, Christoph Georg Baums, Thomas Grunwald, Sebastian Ulbert
Vaccines.2020; 8(1): 113. CrossRef - Next-Generation Whole-Cell Pneumococcal Vaccine
Victor Morais, Esther Texeira, Norma Suarez
Vaccines.2019; 7(4): 151. CrossRef - Gamma-irradiation-killed Streptococcus pneumoniae potently induces the expression of IL-6 and IL-8 in human bronchial epithelial cells
Min Yong Jwa, Eun Byeol Ko, Hyun Young Kim, Sun Kyung Kim, Soyoung Jeong, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Microbial Pathogenesis.2018; 124: 38. CrossRef
- Acute otitis media pneumococcal disease burden and nasopharyngeal colonization in children due to serotypes included and not included in current and new pneumococcal conjugate vaccines
- Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
- Yulan Qiu , Xuemei Zhang , Xinyuan Zhang , Yunjun Mo , Xiaoyu Sun , Jichao Wang , Yibing Yin , Wenchun Xu
- J. Microbiol. 2017;55(10):823-829. Published online September 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-7262-1
- 365 View
- 0 Download
- 5 Crossref
-
Abstract
PDF
-
diseases in children under 5-year-old. Vaccine has been used
as an indispensable strategy to prevent S. pneumoniae infection
for more than 30 years. Our previous studies confirmed
that mucosal immunization with live attenuated strain SPY1
can protect mice against nasopharyngeal colonization of S.
pneumoniae and lethal pneumococcal infection, and the
protective effects are comparable with those induced by commercially
available 23-valent polysaccharide vaccine. However,
live attenuated vaccine SPY1 needs four inoculations to
get satisfactory protective effect, which may increase the risk
of virulence recovery. It is reported that heterologous primeboost
approach is more effective than homologous primeboost
approach. In the present study, to decrease the doses
of live SPY1 and improve the safety of SPY1 vaccine, we immunized
mice with SPY1 and DnaJ protein alternately. Our
results
showed that heterologous prime-boost immunization with SPY1 and DnaJ protein could significantly reduce the colonization of S. pneumoniae in the respiratory tract of mice, and induce stronger Th1 and Th17 cellular immune responses than SPY1 alone. These results indicate heterologous prime-boost immunization method not only elicits better protective effect than SPY1 alone, but also reduces the doses of live SPY1 and decreases the risk of SPY1 vaccine. This work is the first time to study the protective efficiency with two different forms of S. pneumoniae candidate vaccine, and provides a new strategy for the development of S. pneumoniae vaccine. -
Citations
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Luís Cláudio Lima de Jesus, Rhayane Cristina Viegas Santos, Vasco Azevedo
BioDrugs.2025;[Epub] CrossRef - Subcutaneous immunization with the fusion protein ΔA146Ply-SP0148 confers protection against Streptococcus pneumoniae infection
Yao Wang, Lingyin Xia, Guangli Wang, Huifang Lu, Hui Wang, Shilu Luo, Tao Zhang, Song Gao, Jian Huang, Xun Min
Microbial Pathogenesis.2022; 162: 105325. CrossRef - The imbalance of the Th17/Treg axis following equine ascending placental infection
C.E Fedorka, H. El-Sheikh Ali, O.F. Walker, K.E. Scoggin, P. Dini, S.C. Loux, M.H.T. Troedsson, B.A. Ball
Journal of Reproductive Immunology.2021; 144: 103268. CrossRef - Combined prime-boost immunization with systemic and mucosal pneumococcal vaccines based on Pneumococcal surface protein A to enhance protection against lethal pneumococcal infections
Yue Zhang, Xiaonan Guo, Mengze Guo, Xiaorui Chen, Bo Li, Jinfei Yu, Tiejun Gu, Wei Kong, Yongge Wu
Immunologic Research.2019; 67(4-5): 398. CrossRef - Protective Regulatory T Cell Immune Response Induced by Intranasal Immunization With the Live-Attenuated Pneumococcal Vaccine SPY1 via the Transforming Growth Factor-β1-Smad2/3 Pathway
Hongyi Liao, Xiaoqiong Peng, Lingling Gan, Jiafu Feng, Yue Gao, Shenghui Yang, Xuexue Hu, Liping Zhang, Yibing Yin, Hong Wang, Xiuyu Xu
Frontiers in Immunology.2018;[Epub] CrossRef
- Recombinant Molecules as a New Frontier in Mucositis Therapy
Research Support, Non-U.S. Gov'ts
- Pneumococcal wall teichoic acid is required for the pathogenesis of Streptococcus pneumoniae in murine models
- Hongmei Xu , Libin Wang , Jian Huang , Yanqing Zhang , Feng Ma , Jianmin Wang , Wenchun Xu , Xuemei Zhang , Yibing Yin , Kaifeng Wu
- J. Microbiol. 2015;53(2):147-154. Published online January 28, 2015
- DOI: https://doi.org/10.1007/s12275-015-4616-4
- 386 View
- 0 Download
- 4 Crossref
-
Abstract
- Pneumococcal asymptomatic colonization of the respiratory tracts is a major risk for invasive pneumococcal disease. We have previously shown that pneumococcal wall teichoic acid (WTA) was involved in pneumococcal infection of sepsis and adherence to epithelial and endothelial cells. In this study, we investigated the contribution of pneumococcal WTA to bacterial colonization and dissemination in murine models. The result showed that nasopharynx colonizing D39 bacterial cells have a distinct phenotype showing an increased exposure of teichoic acids relative to medium-grown bacteria. The WTA-deficient mutants were impaired in their colonization to the nasopharynx and lungs, and led to a mild inflammation in the lungs at 36 h post-inoculation. Pretreatment of the murine nares with WTA reduced the ability of wild type D39 bacteria to colonize the nasopharynx. In addition, the WTA-deficient strain was impaired in its ability to invade the blood and brain following intranasal administration. WTA-deficient D39 strain was reduced in C3 deposition but was more susceptible to the killing by the neutrophils as compared with its parent strain. Our results also demonstrated that the WTA enhanced pneumococcal colonization and dissemination independently of the host strains. These results indicate that WTA plays an important role in pneumococcal pathogenesis, both in colonization and dissemination processes.
-
Citations
Citations to this article as recorded by- Insight into the structure, biosynthesis, isolation method and biological function of teichoic acid in different gram-positive microorganisms: A review
Jiarun Han, Xin Zhao, Xilian Zhao, Ping Li, Qing Gu
International Journal of Biological Macromolecules.2023; 253: 126825. CrossRef - spd1672, a novel in vivo-induced gene, affects inflammatory response in a murine model of Streptococcus pneumoniae infection
Lingling Gan, Xuemei Zhang, Xiuyu Xu, Wenchun Xu, Chang Lu, Jin Cui, Hong Wang
Canadian Journal of Microbiology.2018; 64(6): 401. CrossRef - Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
Nathalie Heß, Franziska Waldow, Thomas P. Kohler, Manfred Rohde, Bernd Kreikemeyer, Alejandro Gómez-Mejia, Torsten Hain, Dominik Schwudke, Waldemar Vollmer, Sven Hammerschmidt, Nicolas Gisch
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Robert T Gale, Eric D Brown
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- Insight into the structure, biosynthesis, isolation method and biological function of teichoic acid in different gram-positive microorganisms: A review
- Changes in Gene Expression of Actinobacillus pleuropneumoniae in Response to Anaerobic Stress Reveal Induction of Central Metabolism and Biofilm Formation
- Lu Li , Jiawen Zhu , Kui Yang , Zhuofei Xu , Ziduo Liu , Rui Zhou
- J. Microbiol. 2014;52(6):473-481. Published online April 11, 2014
- DOI: https://doi.org/10.1007/s12275-014-3456-y
- 350 View
- 1 Download
- 17 Crossref
-
Abstract
PDF
- Actinobacillus pleuropneumoniae is an important porcine respiratory pathogen causing great economic losses in the pig industry worldwide. Oxygen deprivation is a stress that A. pleuropneumoniae will encounter during both early infection and the later, persistent stage. To understand modulation of A. pleuropneumoniae gene expression in response to the stress caused by anaerobic conditions, gene expression profiles under anaerobic and aerobic conditions were compared in this study. The microarray results showed that 631 genes (27.7% of the total ORFs) were differentially expressed in anaerobic conditions. Many genes encoding proteins involved in glycolysis, carbon source uptake systems, pyruvate metabolism, fermentation and the electron respiration transport chain were up-regulated. These changes led to an increased amount of pyruvate, lactate, ethanol and acetate in the bacterial cells as confirmed by metabolite detection. Genes encoding proteins involved in cell surface structures, especially biofilm formation, peptidoglycan biosynthesis and lipopolysaccharide biosynthesis were up-regulated as well. Biofilm formation was significantly enhanced under anaerobic conditions. These results indicate that induction of central metabolism is important for basic survival of A. pleuropneumoniae after a shift to an anaerobic environment. Enhanced biofilm formation may contribute to the persistence of this pathogen in the damaged anaerobic host tissue and also in the early colonization stage. These discoveries give new insights into adaptation mechanisms of A. pleuropneumoniae in response to environmental stress.
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Mycobacteroides abscessus
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Critical Reviews in Microbiology.2025; 51(5): 792. CrossRef - Effect of epinephrine on growth characteristics of Actinobacillus pleuropneumoniae field isolates
Elena Schreiber, Fritjof Freise, Nicole de Buhr, Isabel Hennig-Pauka
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Malaysian Applied Biology.2025; 54(3): 15. CrossRef - The morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm
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Veterinary Research.2023;[Epub] CrossRef - The Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P
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Infection and Immunity.2022;[Epub] CrossRef - Identification of FtpA, a Dps-Like Protein Involved in Anti-Oxidative Stress and Virulence in Actinobacillus pleuropneumoniae
Hao Tang, Qiuhong Zhang, Weiyao Han, Zhenyue Wang, Siqi Pang, Han Zhu, Kangning Tan, Xiao Liu, Paul R. Langford, Qi Huang, Rui Zhou, Lu Li, Julie A. Maupin-Furlow
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Mycobacteroides abscessus
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- Serotype-Independent Protection against Pneumococcal Infections Elicited by Intranasal Immunization with Ethanol-Killed Pneumococcal Strain, SPY1
- Xiuyu Xu , Jiangping Meng , Yiping Wang , Jie Zheng , Kaifeng Wu , Xuemei Zhang , Yibing Yin , Qun Zhang
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- 346 View
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- 18 Crossref
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Abstract
PDF
- The 23-valent polysaccharide vaccine and the 7-valent pneumococcal conjugate vaccine are licensed vaccines that protect against pneumococcal infections worldwide. However, the incidence of pneumococcal diseases remains high in lowincome countries. Whole-cell vaccines with high safety and strong immunogenicity may be a favorable choice. We previously obtained a capsule-deficient Streptococcus pneumoniae mutant named SPY1 derived from strain D39. As an attenuated live pneumococcal vaccine, intranasal immunization with SPY1 elicits broad serotype-independent protection against pneumococcal infection. In this study, for safety consideration, we inactivated SPY1 with 70% ethanol and intranasally immunized BALB/c mice with killed SPY1 plus cholera toxin adjuvant for four times. Results showed that intranasal immunization with inactivated SPY1 induced strong humoral and cellular immune responses. Intranasal immunization with inactivated SPY1 plus cholera toxin adjuvant elicited effective serotype-independent protection against the colonization of pneumococcal strains 19F and 4 as well as lethal infection of pneumococcal serotypes 2, 3, 14, and 6B. The protection rates provided by inactivated SPY1 against lethal pneumococcal infection were comparable to those of currently used polysaccharide vaccines. In addition, vaccinespecific B-cell and T-cell immune responses mediated the protection elicited by SPY1. In conclusion, the 70% ethanolinactivated pneumococcal whole-cell vaccine SPY1 is a potentially safe and less complex vaccine strategy that offers broad protection against S. pneumoniae.
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Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef
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Streptococcus pneumoniae
serotype distribution in low- and middle-income countries of South Asia: Do we need to revisit the pneumococcal vaccine strategy?
- Characterization of Streptococcus pneumoniae N-Acetylglucosamine-6-Phosphate Deacetylase as a Novel Diagnostic Marker
- Chi-Won Choi , Hee-Young An , Yong Ju Lee , Yeol Gyun Lee , Sung Ho Yun , Edmond Changkyun Park , Yeonhee Hong , Gun-Hwa Kim , Jae-Eun Park , Sun Jong Baek , Hyun Sik Kim , Seung Il Kim
- J. Microbiol. 2013;51(5):659-664. Published online October 31, 2013
- DOI: https://doi.org/10.1007/s12275-013-3451-8
- 331 View
- 0 Download
- 4 Crossref
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Abstract
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- The identification of novel diagnostic markers of pathogenic bacteria is essential for improving the accuracy of diagnoses and for developing targeted vaccines. Streptococcus pneumoniae is a significant human pathogenic bacterium that causes pneumonia. N-acetylglucosamine-6-phosphate deacetylase (NagA) was identified in a protein mixture secreted by S. pneumoniae and its strong immunogenicity was confirmed in an immuno-proteomic assay against the anti-serum of the secreted protein mixture. In this study, recombinant S. pneumoniae NagA protein was expressed and purified to analyze its protein characteristics, immunospecificity, and immunogenicity, thereby facilitating its evaluation as a novel diagnostic marker for S. pneumoniae. Mass spectrometry analysis showed that S. pneumoniae NagA contains four internal disulfide bonds and that it does not undergo posttranslational modification. S. pneumoniae NagA antibodies successfully detected NagA from different S. pneumoniae strains, whereas NagA from other pathogenic bacteria species was not detected. In addition, mice infected with S. pneumoniae generated NagA antibodies in an effective manner. These results suggest that NagA has potential as a novel diagnostic marker for S. pneumoniae because of its high immunogenicity and immunospecificity.
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Citations
Citations to this article as recorded by- Multi-omic profiling to assess the effect of iron starvation inStreptococcus pneumoniaeTIGR4
Irene Jiménez-Munguía, Mónica Calderón-Santiago, Antonio Rodríguez-Franco, Feliciano Priego-Capote, Manuel J. Rodríguez-Ortega
PeerJ.2018; 6: e4966. CrossRef - Mycoplasma fermentans deacetylase promotes mammalian cell stress tolerance
Qingzhou Cheng, Lijuan Wu, Rongfu Tu, Jun Wu, Wenqian Kang, Tong Su, Runlei Du, Wenbin Liu
Microbiological Research.2017; 201: 1. CrossRef - Serotype IV Sequence Type 468 Group BStreptococcusNeonatal Invasive Disease, Minnesota, USA
Sarah Teatero, Patricia Ferrieri, Nahuel Fittipaldi
Emerging Infectious Diseases.2016; 22(11): 1937. CrossRef - Mass Spectrometry in Clinical Microbiology and Infectious Diseases
Frank Fleurbaaij, Hans C. van Leeuwen, Oleg I. Klychnikov, Ed J. Kuijper, Paul J. Hensbergen
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- Multi-omic profiling to assess the effect of iron starvation inStreptococcus pneumoniaeTIGR4
- Screening and Identification of ClpE Interaction Proteins in Streptococcus pneumoniae by a Bacterial Two-Hybrid System and Co-immunoprecipitation
- WenJuan Yan , YingYing Cai , Qun Zhang , YuSi Liu , WenChun Xu , YiBing Yin , YuJuan He , Hong Wang , XueMei Zhang
- J. Microbiol. 2013;51(4):453-460. Published online August 30, 2013
- DOI: https://doi.org/10.1007/s12275-013-3001-4
- 277 View
- 0 Download
- 4 Crossref
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Abstract
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- Hsp100/Clp proteins have crucial functions in the protein quality control, stress tolerance, and virulence of many pathogenic bacteria. ClpE is an important virulence factor involved in adherence and invasion in Streptococcus pneumoniae. To explore the underlying mechanism, we screened ClpE interaction proteins using a bacterial two-hybrid system and co-immunoprecipitation. We used ClpE as bait and constructed the pBT-ClpE bait plasmid for two-hybrid screening. Then, we constructed ClpE::GFP fusion for co-immunoprecipitation screening using anti-GFP monoclonal antibody. We obtained eight potential ClpE interaction proteins, including carbamoyl-phosphate synthase, pyruvate oxidase (SpxB), phosphoenolpyruvate-protein phosphotransferase, aminopeptidase N (pepN), L-lactate dehydrogenase, ribosomal protein S4, sensor histidine kinase (SPD_2019), and FtsW (a cell division protein). FtsW, SpxB, pepN, and SPD_2019 were confirmed to interact with ClpE using Bacterial Two-hybrid or Co-immunoprecipitation. Morphologic observations found that ΔclpE strain existed in abnormal division. β-Galactosidase activity assay suggested that ClpE contributed to the degradation of FtsW. Furthermore, FtsW could be induced by heat shock. The results suggested that ClpE might affect cell division by regulating the level of FtsW. These data may provide new insights in studying the role of ClpE in S. pneumoniae.
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Citations
Citations to this article as recorded by- Legume nodulation and nitrogen fixation require interaction of DnaJ-like protein and lipid transfer protein
Dasong Chen, Dongzhi Li, Ziqi Li, Yuting Song, Qingsong Li, Lihong Wang, Donglai Zhou, Fuli Xie, Youguo Li
Plant Physiology.2023; 193(3): 2164. CrossRef - Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling
Ling Wang, Xuemei Zhang, Guangying Wu, Yuhong Qi, Jinghui Zhang, Jing Yang, Hong Wang, Wenchun Xu
Journal of Microbiology.2020; 58(4): 330. CrossRef - IcmF and DotU are required for the virulence of Acidovorax oryzae strain RS-1
Bin Li, Xiaoxuan Wang, Jie Chen, He Liu, Khattak Arif Ali, Yanli Wang, Wen Qiu, Guochang Sun
Archives of Microbiology.2018; 200(6): 897. CrossRef - Antibacterial effects of Traditional Chinese Medicine monomers against Streptococcus pneumoniae via inhibiting pneumococcal histidine kinase (VicK)
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Frontiers in Microbiology.2015;[Epub] CrossRef
- Legume nodulation and nitrogen fixation require interaction of DnaJ-like protein and lipid transfer protein
- Identification of Conserved Surface Proteins as Novel Antigenic Vaccine Candidates of Actinobacillus pleuropneumoniae
- Xiabing Chen , Zhuofei Xu , Lu Li , Huanchun Chen , Rui Zhou
- J. Microbiol. 2012;50(6):978-986. Published online December 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2214-2
- 288 View
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- 12 Crossref
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Abstract
PDF
- Actinobacillus pleuropneumoniae is an important swine respiratory pathogen causing great economic losses worldwide. Identification of conserved surface antigenic proteins is helpful for developing effective vaccines. In this study, a genome-wide strategy combined with bioinformatic and experimental approaches, was applied to discover and characterize surface-associated immunogenic proteins of A. pleuropneumoniae. Thirty nine genes encoding outer membrane proteins (OMPs) and lipoproteins were identified by comparative genomics and gene expression profiling as beinghighly conserved and stably transcribed in the different serotypes of A. pleuropneumoniae reference strains. Twelve of these conserved proteins were successfully expressed in Escherichia coli and their immunogenicity was estimated by homologous challenge in the mouse model, and then three of these proteins (APJL_0126, HbpA and OmpW) were further tested in the natural host (swine) by homologous and heterologous challenges. The results showed that these proteins could induce high titers of antibodies, but vaccination with each protein individually elicited low protective immunity against A. pleuropneumoniae. This study gives novel insights into immunogenicity of the conserved OMPs and lipoproteins of A. pleuropneumoniae. Although none of the surface proteins characterized in this study could individually induce effective protective immunity against A. pleuropneumoniae, they are potential candidates for subunit vaccines in combination with Apx toxins.
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Citations
Citations to this article as recorded by- Identification of candidate vaccine antigens using 2-D gel electrophoresis and immunoproteomics for cross protection against Glaesserella parasuis
Samantha J. Hau, Kirsten C. Eberle, Jarlath E. Nally, Daniel W. Nielsen, John D. Lippolis, Susan L. Brockmeier
Veterinary Microbiology.2025; 307: 110594. CrossRef - Review of advanced research on swine Actinobacillus pleuropneumoniae vaccine development strategy
Adehanom Baraki Tesfaye, Rui Han, Zhengyu Tao, Liuchao You, Jiayao Zhu, Pengcheng Gao, Lei Fu, Yuefeng Chu
Frontiers in Immunology.2025;[Epub] CrossRef - De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies
Jinyue Yang, Xueting Zhang, Junhua Dong, Qian Zhang, Erchao Sun, Cen Chen, Zhuangxia Miao, Yifei Zheng, Nan Zhang, Pan Tao
Frontiers in Immunology.2023;[Epub] CrossRef - Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody
Geyan Liu, Kang Wang, Zhen Yang, Xiaoyu Tang, Yung-Fu Chang, Ke Dai, Xinwei Tang, Bangdi Hu, Yiwen Zhang, Sanjie Cao, Xiaobo Huang, Qigui Yan, Rui Wu, Qin Zhao, Senyan Du, Xintian Wen, Yiping Wen
International Journal of Molecular Sciences.2023; 24(10): 8638. CrossRef - Proteomic and immunoproteomic insights into the exoproteome of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia
Stelli G. Stancheva, Janna Frömbling, Elena L. Sassu, Isabel Hennig-Pauka, Andrea Ladinig, Wilhelm Gerner, Tom Grunert, Monika Ehling-Schulz
Microbial Pathogenesis.2022; 172: 105759. CrossRef - Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge
Yurou Cao, Lulu Gao, Li Zhang, Lixiang Zhou, Jihong Yang, Lingfu Deng, Jin Zhao, Chao Qi, Jinlin Liu
Scientific Reports.2020;[Epub] CrossRef - The roles of flp1 and tadD in Actinobacillus pleuropneumoniae pilus biosynthesis and pathogenicity
Tingting Li, Qiuhong Zhang, Rong Wang, Sihua Zhang, Jie Pei, Yaokun Li, Lu Li, Rui Zhou
Microbial Pathogenesis.2019; 126: 310. CrossRef - Recombinant ApxIV protein enhances protective efficacy againstActinobacillus pleuropneumoniaein mice and pigs
H.-C. Wu, P.-H. Yeh, K.-J. Hsueh, W.-J. Yang, C.-Y. Chu
Journal of Applied Microbiology.2018; 124(6): 1366. CrossRef - New trends in innovative vaccine development against Actinobacillus pleuropneumoniae
Abraham Loera-Muro, Carlos Angulo
Veterinary Microbiology.2018; 217: 66. CrossRef - A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model
Kui Xu, Qin Zhao, Xintian Wen, Rui Wu, Yiping Wen, Xiaobo Huang, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Yung-Fu Chang, Sanjie Cao, Utpal Pal
PLOS ONE.2018; 13(1): e0191286. CrossRef - Identification and characterization of a novel stress-responsive outer membrane protein Lip40 from Actinobacillus pleuropneumoniae
Xuehe Hu, Hao Yan, Ke Liu, Jiansheng Hu, Chao Qi, Jihong Yang, Yanli Liu, Jin Zhao, Jinlin Liu
BMC Biotechnology.2015;[Epub] CrossRef - Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
Luhua Zhang, Yiping Wen, Ying Li, Xingliang Wei, Xuefeng Yan, Xintian Wen, Rui Wu, Xiaobo Huang, Yong Huang, Qigui Yan, Mafeng Liu, Sanjie Cao
Proteome Science.2014;[Epub] CrossRef
- Identification of candidate vaccine antigens using 2-D gel electrophoresis and immunoproteomics for cross protection against Glaesserella parasuis
- SP0454, A Putative Threonine Dehydratase, Is Required For Pneumococcal Virulence In Mice
- WenJuan Yan , Hong Wang , WenChun Xu , KaiFeng Wu , Run Yao , XiuYu Xu , Jie Dong , YanQing Zhang , Wen Zhong , XueMei Zhang
- J. Microbiol. 2012;50(3):511-517. Published online June 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2014-8
- 255 View
- 0 Download
- 5 Crossref
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Abstract
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- Increasing pressure in antibiotic resistance and the requirement for the design of new vaccines are the objectives of clarifying the putative virulence factors in pneumococcal infection. In this study, the putative threonine dehydratase sp0454 was inactivated by erythromycin-resistance cassette replacement in Streptococcus pneumoniae CMCC 31203 strain. The sp0454 mutant was tested for cell growth, adherence, colonization, and virulence in a murine model. The Δsp0454 mutant showed decreased ability for colonization and impaired ability to adhere to A549 cells. However, the SP0454 polypeptide or its antiserum did not affect pneumococcal CMCC 31203 adhesion to A549 cells. The sp0454 deletion mutant was less virulent in a murine intranasal infection model. Real-time RT-PCR analysis revealed significant decrease of the pneumococcal surface antigen A expression in the sp0454 mutant. These results suggest that SP0454 contributes to virulence and colonization, which could be explained in part by modulating the expression of other virulence factors, such as psaA in pneumococcal infection.
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Citations
Citations to this article as recorded by- Dual RNA-Seq of Flavobacterium psychrophilum and Its Outer Membrane Vesicles Distinguishes Genes Associated with Susceptibility to Bacterial Cold-Water Disease in Rainbow Trout (Oncorhynchus mykiss)
Pratima Chapagain, Ali Ali, Mohamed Salem
Pathogens.2023; 12(3): 436. CrossRef - Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Journal of Microbiology.2017; 55(10): 823. CrossRef - Mucosal Immunization with the Live Attenuated Vaccine SPY1 Induces Humoral and Th2-Th17-Regulatory T Cell Cellular Immunity and Protects against Pneumococcal Infection
Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef - A Novel Protein, RafX, Is Important for Common Cell Wall Polysaccharide Biosynthesis in Streptococcus pneumoniae: Implications for Bacterial Virulence
Kaifeng Wu, Jian Huang, Yanqing Zhang, Wenchun Xu, Hongmei Xu, Libin Wang, Ju Cao, Xuemei Zhang, Yibing Yin
Journal of Bacteriology.2014; 196(18): 3324. CrossRef - Serotype-independent protection against pneumococcal infections elicited by intranasal immunization with ethanol-killed pneumococcal strain, SPY1
Xiuyu Xu, Jiangping Meng, Yiping Wang, Jie Zheng, Kaifeng Wu, Xuemei Zhang, Yibing Yin, Qun Zhang
Journal of Microbiology.2014; 52(4): 315. CrossRef
- Dual RNA-Seq of Flavobacterium psychrophilum and Its Outer Membrane Vesicles Distinguishes Genes Associated with Susceptibility to Bacterial Cold-Water Disease in Rainbow Trout (Oncorhynchus mykiss)
- Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection
- Li Jun Zhang , Li Jun Zhang , Wei Quan , Bei Bei Wang , Bing Ling Shen , Teng Teng Zhang , Yi Kang
- J. Microbiol. 2011;49(5):834-840. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-1051-z
- 303 View
- 0 Download
- 7 Crossref
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Abstract
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- This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.
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Citations
Citations to this article as recorded by- Berberine and its nanoformulations and extracts: potential strategies and future perspectives against multi-drug resistant bacterial infections
Xue Yang, Yanfen Wang, Ling Li, Daiyan Tang, Zhong Yan, MingYan Li, Jiayi Jiang, Dongming Bi
Frontiers in Microbiology.2025;[Epub] CrossRef - Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens
Effie E. Bastounis, Prathima Radhakrishnan, Christopher K. Prinz, Julie A. Theriot
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef - Berberine: Pathways to protect neurons
Xiaorui Lin, Nan Zhang
Phytotherapy Research.2018; 32(8): 1501. CrossRef - Berberine inhibits Chlamydia pneumoniae infection-induced vascular smooth muscle cell migration through downregulating MMP3 and MMP9 via PI3K
Lu Ma, Lijun Zhang, Beibei Wang, Junyan Wei, Jingya Liu, Lijun Zhang
European Journal of Pharmacology.2015; 755: 102. CrossRef - Rational Design of Berberine-Based FtsZ Inhibitors with Broad-Spectrum Antibacterial Activity
Ning Sun, Fung-Yi Chan, Yu-Jing Lu, Marco A. C. Neves, Hok-Kiu Lui, Yong Wang, Ka-Yan Chow, Kin-Fai Chan, Siu-Cheong Yan, Yun-Chung Leung, Ruben Abagyan, Tak-Hang Chan, Kwok-Yin Wong, Dirk-Jan Scheffers
PLoS ONE.2014; 9(5): e97514. CrossRef -
Chlamydia pneumoniae infection induces vascular smooth muscle cell migration via Rac1 activation
Junxia Zhang, Haiwei Wang, Lijun Zhang, Tengteng Zhang, Beibei Wang, Xiankui Li, Junyan Wei, Lijun Zhang
Journal of Medical Microbiology .2014; 63(2): 155. CrossRef - Chlamydia pneumoniae Infection Promotes Vascular Smooth Muscle Cell Migration through a Toll-Like Receptor 2-Related Signaling Pathway
Beibei Wang, Lijun Zhang, Tengteng Zhang, Haiwei Wang, Junxia Zhang, Junyan Wei, Bingling Shen, Xin Liu, Zhelong Xu, Lijun Zhang, R. P. Morrison
Infection and Immunity.2013; 81(12): 4583. CrossRef
- Berberine and its nanoformulations and extracts: potential strategies and future perspectives against multi-drug resistant bacterial infections
- Molecular Analysis of a Prolonged Spread of Klebsiella pneumoniae Co-producing DHA-1 and SHV-12 β-Lactamases
- Young Kyung Yoon , Hye Won Cheong , Hyunjoo Pai , Kyoung Ho Roh , Jeong Yeon Kim , Dae Won Park , Jang Wook Sohn , Seung Eun Lee , Byung Chul Chun , Hee Sun Sim , Min Ja Kim
- J. Microbiol. 2011;49(3):363-368. Published online June 30, 2011
- DOI: https://doi.org/10.1007/s12275-011-0491-9
- 232 View
- 0 Download
- 4 Crossref
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Abstract
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- The study investigated molecular mechanisms for prolonged nosocomial spread of multidrug-resistant Klebsiella pneumoniae co-producing plasmid-mediated AmpC β-lactamase DHA-1 and extended-spectrum β-lactamase SHV-12. Forty-eight clinical isolates of K. pneumonia, resistant to the extended-spectrum cephalosporins, were collected in a 750-bed university hospital over a year. The isolates were characterized for PCR-based β-lactamase genotypes, isoelectric focusing and pulsed-field gel electrophoresis (PFGE) profiles. Resistance transfer was performed by plasmid conjugation and confirmed by a duplex-PCR and Southern hybridization. On β-lactamase typing, the strains producing only the DHA-1 enzyme (n=17) or co-producing DHA-1 and SHV-12 enzymes (n=15) were predominant. Judging from a one year-distribution of PFGE profiles, the co-producer was spread primarily with single clonal expansion of the PFGE-type A with subtypes (n=14), whereas the strains producing only DHA-1 enzyme were spread simultaneously with the PFGE-type A (n=11) and other PFGE types (n=6). Transconjugants of the co-producers were confirmed to harbor either both blaDHA-1 and blaSHV-12 or only the blaDHA-1. In conclusion, this study indicated that the persistent nosocomial spread of multidrug-resistant K. pneumoniae strains was primarily associated with expansion of a clone harboring both the blaDHA-1 and blaSHV-12 or the blaDHA-1 only, and to a lesser extent with the horizontal transfer of the resistant plasmids. Our observations have clinical implication for the control and prevention of nosocomial dissemination of multidrug-resistant K. pneumoniae strains.
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Citations
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Min Kyeong Cha, Cheol-In Kang, So Hyun Kim, Doo Ryeon Chung, Kyong Ran Peck, Nam Yong Lee, Jae-Hoon Song
Microbial Drug Resistance.2018; 24(7): 1002. CrossRef - Role of piperacillin/tazobactam as a carbapenem-sparing antibiotic for treatment of acute pyelonephritis due to extended-spectrum β-lactamase-producing Escherichia coli
Young Kyung Yoon, Jong Hun Kim, Jang Wook Sohn, Kyung Sook Yang, Min Ja Kim
International Journal of Antimicrobial Agents.2017; 49(4): 410. CrossRef - Emergence of serotype K1 Klebsiella pneumoniae ST23 strains co-producing the plasmid-mediated AmpC beta-lactamase DHA-1 and an extended-spectrum beta-lactamase in Korea
Hae Suk Cheong, Doo Ryeon Chung, Chaeyoeng Lee, So Hyun Kim, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
Antimicrobial Resistance & Infection Control.2016;[Epub] CrossRef - Effects of Group 1 versus Group 2 Carbapenems on the Susceptibility of Acinetobacter baumannii to Carbapenems: A Before and After Intervention Study of Carbapenem-Use Stewardship
Young Kyung Yoon, Kyung Sook Yang, Seung Eun Lee, Hyun Jeong Kim, Jang Wook Sohn, Min Ja Kim, Mark Alexander Webber
PLoS ONE.2014; 9(6): e99101. CrossRef
- High Prevalence of CTX-M-15-Type Extended-Spectrum β-Lactamase Among AmpC β-Lactamase-Producing Klebsiella pneumoniae Isolates Causing Bacteremia in Korea
- Predicting Genetic Traits and Epitope Analysis of apxIVA in Actinobacillus pleuropneumoniae
- Min-Kyoung Shin , Seung-Bin Cha , Won-Jung Lee , Han Sang Yoo
- J. Microbiol. 2011;49(3):462-468. Published online June 30, 2011
- DOI: https://doi.org/10.1007/s12275-011-0449-y
- 277 View
- 1 Download
- 7 Crossref
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Abstract
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- Actinobacillus pleuropneumoniae causes a severe hemorrhagic pneumonia in pigs. Fifteen serotypes of A. pleuropneumoniae express four different Apx toxins that belong to the pore-forming repeats-in-toxin (RTX) group of toxins. ApxIV, which is conserved and up-regulated in vivo, could be an excellent candidate for the development of a protective cross-serotype immunity vaccine, and could aid in the differential diagnosis of diseases caused by A. pleuropneumoniae. We identified and sequenced apxIVA from A. pleuropneumoniae serotype 2 isolated in Korea (Kor-ApxIVA). The Kor-ApxIVA was closely related to Switzerland (AF021919), China (CP000687), and China (GQ332268), showing 98.6%, 98.4%, and 97.2% amino acid homology, respectively. The level of amino acid homology, however, was higher than the nucleotide homology. The structural characteristics of ApxIVA showed RTX proteins, including N-terminal hydrophobic domains, signature sequences for potential acylation sites, and repeated glycine-rich nonapeptides in the C-terminal region of the protein. Thirty glycine-rich nonapeptides with the consensus sequence, L/V-X-G-G-X-G-N/D-D-X, were found in the C-terminus of the Kor-ApxIVA. In addition, the Kor-ApxIVA was predicted for the linear B-cell epitopes and conserved domains with determined peptide sequences. This genetic analysis of the Kor-ApxIVA might be an important foundation for future biological and functional research on ApxIVA.
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Citations
Citations to this article as recorded by- Review of advanced research on swine Actinobacillus pleuropneumoniae vaccine development strategy
Adehanom Baraki Tesfaye, Rui Han, Zhengyu Tao, Liuchao You, Jiayao Zhu, Pengcheng Gao, Lei Fu, Yuefeng Chu
Frontiers in Immunology.2025;[Epub] CrossRef - Rapid detection of Actinobacillus pleuropneumoniae targeting the apxIVA gene for diagnosis of contagious porcine pleuropneumonia in pigs by polymerase spiral reaction
R. Sarkar, P. Roychoudhury, S. Kumar, S. Dutta, N. Konwar, P. K. Subudhi, T. K. Dutta
Letters in Applied Microbiology.2022; 75(2): 442. CrossRef - Development ofActinobacillus pleuropneumoniaeApxI, ApxII, and ApxIII-specific ELISA methods for evaluation of vaccine efficiency
Myunghwan Jung, Hokeun Won, Min-Kyoung Shin, Myung Whan Oh, Soojin Shim, Injoong Yoon, Han Sang Yoo
Journal of Veterinary Science.2019;[Epub] CrossRef - Carbon source utilisation and evaluation of the Biolog system in the identification of Actinobacillus pleuropneumoniae
László Makrai, Rita Sárközi, László Fodor
Acta Veterinaria Hungarica.2019; 67(3): 327. CrossRef - So sánh khả năng dự đoán chất lượng nước sông Đồng Nai bằng mạng nơ ron nhân tạo và lý thuyết xám
Nguyễn Hiền Thân, Chế Đình Lý, Phạm Văn Tất
Can Tho University, Journal of Science.2017; Môi trường: 120. CrossRef - Prevalence and Characterization ofActinobacillus pleuropneumoniaeIsolated from Korean Pigs
Ki-Eun Lee, Hwan-Won Choi, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang
Journal of Bacteriology and Virology.2015; 45(1): 19. CrossRef - Identification and Characterization of the Insecticidal Toxin “Makes Caterpillars Floppy” in Photorhabdus temperata M1021 Using a Cosmid Library
Ihsan Ullah, Eun-Kyung Jang, Min-Sung Kim, Jin-Ho Shin, Gun-Seok Park, Abdur Khan, Sung-Jun Hong, Byung-Kwon Jung, JungBae Choi, YeongJun Park, Yunyoung Kwak, Jae-Ho Shin
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- NOTE] Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach
- Chi-Won Choi , Sung-Ho Yun , Sang-Oh Kwon , Sun-Hee Leem , Jong-Soon Choi , Chi-Young Yun , Seung Il Kim
- J. Microbiol. 2010;48(6):872-876. Published online January 9, 2011
- DOI: https://doi.org/10.1007/s12275-010-0220-9
- 176 View
- 0 Download
- 9 Crossref
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Abstract
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- In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferse systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.
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Enzong Xiao, Weimin Sun, Jinmei Deng, Li Shao, Zengping Ning, Tangfu Xiao
Plant and Soil.2023; 493(1-2): 589. CrossRef - Efficient profiling of detergent-assisted membrane proteome in cyanobacteria
Jong-Soon Choi, Yun Hwan Park, Jeong Hyun Oh, Sooyong Kim, Joseph Kwon, Yoon-E Choi
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María de la Luz Mohedano, Pasquale Russo, Vivian de los Ríos, Vittorio Capozzi, Pilar Fernández de Palencia, Giuseppe Spano, Paloma López
Open Biology.2014; 4(2): 130154. CrossRef - Influence of Impaired Lipoprotein Biogenesis on Surface and Exoproteome of Streptococcus pneumoniae
Thomas Pribyl, Martin Moche, Annette Dreisbach, Jetta J.E. Bijlsma, Malek Saleh, Mohammed R. Abdullah, Michael Hecker, Jan Maarten van Dijl, Dörte Becher, Sven Hammerschmidt
Journal of Proteome Research.2014; 13(2): 650. CrossRef - Identification of Potential New Protein Vaccine Candidates through Pan-Surfomic Analysis of Pneumococcal Clinical Isolates from Adults
Alfonso Olaya-Abril, Irene Jiménez-Munguía, Lidia Gómez-Gascón, Ignacio Obando, Manuel J. Rodríguez-Ortega, Paulo Lee Ho
PLoS ONE.2013; 8(7): e70365. CrossRef - Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker
Chi-Won Choi, Hee-Young An, Yong Ju Lee, Yeol Gyun Lee, Sung Ho Yun, Edmond Changkyun Park, Yeonhee Hong, Gun-Hwa Kim, Jae-Eun Park, Sun Jong Baek, Hyun Sik Kim, Seung Il Kim
Journal of Microbiology.2013; 51(5): 659. CrossRef - Reevaluation of the Harboe Assay as a Standardized Method of Assessment for the Hemolytic Performance of Ventricular Assist Devices
Chris H.H. Chan, Andrew Hilton, Graham Foster, Karl Hawkins
Artificial Organs.2012; 36(8): 724. CrossRef - Application of subproteomics in the characterization of Gram-positive bacteria
Xiao-Yan Yang, Jie Lu, Xuesong Sun, Qing-Yu He
Journal of Proteomics.2012; 75(10): 2803. CrossRef - Analysis of Streptococcus pneumoniae secreted antigens by immuno-proteomic approach
Chi-Won Choi, Yeol Gyun Lee, Sang-Oh Kwon, Hye-Yeon Kim, Je Chul Lee, Young-Ho Chung, Chi-Young Yun, Seung Il Kim
Diagnostic Microbiology and Infectious Disease.2012; 72(4): 318. CrossRef
- The potential linkage between antibiotic resistance genes and microbial functions across soil–plant systems
- Role of Hydrogen Generation by Klebsiella pneumoniae in the Oral Cavity
- Tomoko Kanazuru , Eisuke F. Sato , Kumiko Nagata , Hiroshi Matsui , Kunihiko Watanabe , Emiko Kasahara , Mika Jikumaru , June Inoue , Masayasu Inoue
- J. Microbiol. 2010;48(6):778-783. Published online January 9, 2011
- DOI: https://doi.org/10.1007/s12275-010-0149-z
- 314 View
- 0 Download
- 12 Crossref
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Abstract
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- Some gastrointestinal bacteria synthesize hydrogen (H2) by fermentation. Despite the presence of bactericidal factors in human saliva, a large number of bacteria also live in the oral cavity. It has never been shown that oral bacteria also produce H2 or what role H2 might play in the oral cavity. It was found that a significant amount of H2 is synthesized in the oral cavity of healthy human subjects, and that its generation is enhanced by the presence of glucose but inhibited by either teeth brushing or sterilization with povidone iodine. These observations suggest the presence of H2-generating bacteria in the oral cavity. The screening of commensal bacteria in the oral cavity revealed that a variety of anaerobic bacteria generate H2. Among them, Klebsiella pneumoniae (K. pneumoniae) generated significantly large amounts of H2 in the presence of glucose. Biochemical analysis revealed that various proteins in K. pneumoniae are carbonylated under standard culture conditions, and that oxidative stress induced by the presence of Fe++ and H2O2 increases the number of carbonylated proteins, particularly when their hydrogenase activity is inhibited by KCN. Inhibition of H2 generation markedly suppresses the growth of K. pneumoniae. These observations suggest that H2 generation and/or the reduction of oxidative stress is important for the survival and growth of K. pneumoniae in the oral cavity.
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Citations
Citations to this article as recorded by- Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms
Guoping Ren, Jie Ye, Lu Liu, Andong Hu, Kenneth H. Nealson, Christopher Rensing, Shungui Zhou
Engineering.2025; 47: 194. CrossRef - The oral-gut microbiome axis in inflammatory bowel disease: from inside to insight
Aili Wang, Zihan Zhai, Yiyun Ding, Jingge Wei, Zhiqiang Wei, Hailong Cao
Frontiers in Immunology.2024;[Epub] CrossRef - Targeting bacterial nickel transport with aspergillomarasmine A suppresses virulence-associated Ni-dependent enzymes
David Sychantha, Xuefei Chen, Kalinka Koteva, Gerd Prehna, Gerard D. Wright
Nature Communications.2024;[Epub] CrossRef - The On/Off History of Hydrogen in Medicine: Will the Interest Persist This Time Around?
Tyler W. LeBaron, Kinji Ohno, John T. Hancock
Oxygen.2023; 3(1): 143. CrossRef - Relationships Between Oral Microecosystem and Respiratory Diseases
Jiajia Dong, Wei Li, Qi Wang, Jiahao Chen, Yue Zu, Xuedong Zhou, Qiang Guo
Frontiers in Molecular Biosciences.2022;[Epub] CrossRef -
Methanobrevibacter smithii Archaemia in Febrile Patients With Bacteremia, Including Those With Endocarditis
Michel Drancourt, Kenza Djemai, Frédérique Gouriet, Ghiles Grine, Ahmed Loukil, Marielle Bedotto, Anthony Levasseur, Hubert Lepidi, Jacques Bou-Khalil, Saber Khelaifia, Didier Raoult
Clinical Infectious Diseases.2021; 73(9): e2571. CrossRef - Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists
Stéphane L. Benoit, Robert J. Maier, R. Gary Sawers, Chris Greening
Microbiology and Molecular Biology Reviews.2020;[Epub] CrossRef - Role of Nickel in Microbial Pathogenesis
Robert Maier, Stéphane Benoit
Inorganics.2019; 7(7): 80. CrossRef - Impact of Klebsiella pneumoniae in lower gastrointestinal tract diseases
Christina Parvinder Kaur, Jamuna Vadivelu, Samudi Chandramathi
Journal of Digestive Diseases.2018; 19(5): 262. CrossRef - Site-directed mutagenesis of Campylobacter concisus respiratory genes provides insight into the pathogen’s growth requirements
Stéphane L. Benoit, Robert J. Maier
Scientific Reports.2018;[Epub] CrossRef - Markers of Perioperative Bowel Complications in Colorectal Surgery Patients
Radomír Hyšpler, Alena Tichá, Milan Kaška, Lenka Žaloudková, Lenka Plíšková, Eduard Havel, Zdeněk Zadák
Disease Markers.2015; 2015: 1. CrossRef - Hydrogenase: the next antibiotic target?
Wei Nie, Hao Tang, Zheng Fang, Jiquan Chen, Haili Chen, Qingyu Xiu
Clinical Science.2012; 122(12): 575. CrossRef
- Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms
- Heterogeneous Virulence Potential and High Antibiotic Resistance of Pseudomonas aeruginosa Strains Isolated from Korean Pneumonia Patients
- Mi Young Yoon , Kang-Mu Lee , Seok Hoon Jeong , Jungmin Kim , Sang Sun Yoon
- J. Microbiol. 2010;48(4):518-525. Published online August 20, 2010
- DOI: https://doi.org/10.1007/s12275-010-9388-2
- 308 View
- 0 Download
- 4 Crossref
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Abstract
PDF
- Pseudomonas aeruginosa is an opportunistic human pathogen of clinical importance that causes airway infections in immunocompromised patients. Here, we report the virulence-associated characteristics of strains of P. aeruginosa, isolated from the sputa of 25 Korean pneumonia patients. A high degree of genomic plasticity was observed by random amplified polymorphic DNA genotype analysis, suggesting that the infections were caused by strains with diverse genomic backgrounds. Biofilm formation of each isolate was heterogeneous in terms of their relative motilities. In addition, 48% of isolates were defective in the production of 3-oxo-C12-HSL (PAI-1), a quorum sensing signal molecule. In these strains, PAI-1-dependent elastase production was correspondingly decreased, suggesting that a large number of strains were presumed to be quorum sensing deficient. Multidrug resistance (MDR) was seen in 56% of the isolates tested, and 44% of the MDR strains were resistant to five or more antibiotics. Taken together, our results provide additional insights into the virulence traits of P. aeruginosa clinical isolates, which will aid in treating P. aeruginosa infections in pneumonia patients.
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Citations
Citations to this article as recorded by- Virulence Characteristics and an Action Mode of Antibiotic Resistance in Multidrug-Resistant Pseudomonas aeruginosa
Wontae Hwang, Sang Sun Yoon
Scientific Reports.2019;[Epub] CrossRef - The ferrichrome receptor A as a new target forPseudomonas aeruginosavirulence attenuation
Keehoon Lee, Kang-Mu Lee, Junhyeok Go, Jae-Chan Ryu, Ji-Hwan Ryu, Sang Sun Yoon, Klaus Hantke
FEMS Microbiology Letters.2016; 363(11): fnw104. CrossRef - A single gene of a commensal microbe affects host susceptibility to enteric infection
Mi Young Yoon, Kyung Bae Min, Kang-Mu Lee, Yujin Yoon, Yaeseul Kim, Young Taek Oh, Keehoon Lee, Jongsik Chun, Byung-Yong Kim, Seok-Hwan Yoon, Insuk Lee, Chan Yeong Kim, Sang Sun Yoon
Nature Communications.2016;[Epub] CrossRef - Anaerobiosis-Induced Loss of Cytotoxicity Is Due to Inactivation of Quorum Sensing in Pseudomonas aeruginosa
Kang-Mu Lee, Mi Young Yoon, Yongjin Park, Joon-Hee Lee, Sang Sun Yoon, B. A. McCormick
Infection and Immunity.2011; 79(7): 2792. CrossRef
- Virulence Characteristics and an Action Mode of Antibiotic Resistance in Multidrug-Resistant Pseudomonas aeruginosa
- Virulence Attenuation of Streptococcus pneumoniae clpP Mutant by Sensitivity to Oxidative Stress in Macrophages via an NO-Mediated Pathway
- Chul-Yong Park , Eun-Hye Kim , Sang-Yoon Choi , Thao Dang-Hien Tran , In-Hye Kim , Su-Nam Kim , Suhkneung Pyo , Dong-Kwon Rhee
- J. Microbiol. 2010;48(2):229-235. Published online May 1, 2010
- DOI: https://doi.org/10.1007/s12275-010-9300-0
- 319 View
- 0 Download
- 19 Crossref
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Abstract
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- ClpP protease is essential for virulence and survival under stress conditions in several pathogenic bacteria. The clpP mutation in a murine infection model has demonstrated both attenuation of virulence and a sensitivity to hydrogen peroxide. However, the underlying mechanisms for these changes have not been resolved. Because macrophages play a major role in immune response and activated macrophages can kill microbes via oxygen-dependant mechanisms, we investigated the effect of the clpP mutation on its sensitivity to macrophage-mediated oxygen-dependant mechanisms. The clpP mutant derived from D39 (serotype 2) exhibited a higher sensitivity to oxidative stresses such as reactive oxygen intermediates, reactive nitrogen intermediates, and H2O2, but no sensitivity to osmotic stress (NaCl) and pH. Moreover, viability of the clpP mutant was significantly increased in murine macrophage cells by treatment with S-methylisothiourea sulfate, which inhibits inducible nitric oxide synthase (iNOS) activity and subsequently elicits lower level secretions of nitric oxide (NO). However, viability of wild type was unchanged. Taken together, these results indicate that ClpP is involved in the resistance to oxidative stresses after entrapment by macrophages and subsequently contributes to virulence via NO mediated pathway.
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Citations
Citations to this article as recorded by-
The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in
Streptococcus pneumoniae
Viktor H. Mebus, Supradipta De, Larissa M. Busch, Manuela Gesell Salazar, Rabea Schlüter, Uwe Völker, Sven Hammerschmidt, Dorte Frees, Carlos J. Blondel
Microbiology Spectrum.2025;[Epub] CrossRef - The oxidative stress response of Streptococcus pneumoniae: its contribution to both extracellular and intracellular survival
Mirelys Hernandez-Morfa, Nadia B. Olivero, Victoria E. Zappia, German E. Piñas, Nicolas M. Reinoso-Vizcaino, Melina B. Cian, Mariana Nuñez-Fernandez, Paulo R. Cortes, Jose Echenique
Frontiers in Microbiology.2023;[Epub] CrossRef - Pathogenicity and virulence ofStreptococcus pneumoniae: Cutting to the chase on proteases
Mary E. Marquart
Virulence.2021; 12(1): 766. CrossRef - ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis
Jinxin Zheng, Yang Wu, Zhiwei Lin, Guangfu Wang, Sibo Jiang, Xiang Sun, Haopeng Tu, Zhijian Yu, Di Qu
BMC Microbiology.2020;[Epub] CrossRef - Las proteasas de serina bacterianas y su implicación en la fisiopatología de la infección
Gerardo García-González, Gloria María González, José P. Palma-Nicolás
Revista del Laboratorio Clínico.2019; 12(3): 137. CrossRef - ClpP Protease, a Promising Antimicrobial Target
Carlos Moreno-Cinos, Kenneth Goossens, Irene G. Salado, Pieter Van Der Veken, Hans De Winter, Koen Augustyns
International Journal of Molecular Sciences.2019; 20(9): 2232. CrossRef - Identification and Characterization of Approved Drugs and Drug-Like Compounds as Covalent Escherichia coli ClpP Inhibitors
Elisa Sassetti, Cristina Durante Cruz, Päivi Tammela, Mathias Winterhalter, Koen Augustyns, Philip Gribbon, Björn Windshügel
International Journal of Molecular Sciences.2019; 20(11): 2686. CrossRef - α-Amino Diphenyl Phosphonates as Novel Inhibitors of Escherichia coli ClpP Protease
Carlos Moreno-Cinos, Elisa Sassetti, Irene G. Salado, Gesa Witt, Siham Benramdane, Laura Reinhardt, Cristina D. Cruz, Jurgen Joossens, Pieter Van der Veken, Heike Brötz-Oesterhelt, Päivi Tammela, Mathias Winterhalter, Philip Gribbon, Björn Windshügel, Koe
Journal of Medicinal Chemistry.2019; 62(2): 774. CrossRef - Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence
Huan Wang, Zhongling Yang, Shuo Du, Lin Ma, Yao Liao, Yujie Wang, Ian Toth, Jiaqin Fan
Molecular Plant Pathology.2018; 19(1): 35. CrossRef - Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in Streptococcus pneumoniae D39
John P. Lisher, Ho-Ching Tiffany Tsui, Smirla Ramos-Montañez, Kristy L. Hentchel, Julia E. Martin, Jonathan C. Trinidad, Malcolm E. Winkler, David P. Giedroc, Craig D. Ellermeier
mSphere.2017;[Epub] CrossRef - An ensemble-guided approach identifies ClpP as a major regulator of transcript levels in nitric oxide-stressed Escherichia coli
Jonathan L. Robinson, Mark P. Brynildsen
Metabolic Engineering.2015; 31: 22. CrossRef - Stress responses in Streptococcus species and their effects on the host
Cuong Thach Nguyen, Sang-Sang Park, Dong-Kwon Rhee
Journal of Microbiology.2015; 53(11): 741. CrossRef - Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein from Streptococcus pyogenes
Shabnam Sadoogh Abbasian, Ehsanollah Ghaznavi Rad, Neda Akbari, Mohammad Reza Zolfaghari, Iraj pakzad, Hamid Abtahi
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef - Streptococcus pneumoniae and reactive oxygen species: an unusual approach to living with radicals
Hasan Yesilkaya, Vahid Farshchi Andisi, Peter W. Andrew, Jetta J.E. Bijlsma
Trends in Microbiology.2013; 21(4): 187. CrossRef - Streptococcus pneumoniae ClpP protease induces apoptosis via caspase-independent pathway in human neuroblastoma cells: Cytoplasmic relocalization of p53
Jun-Oh Lee, Ji-Yun Kim, Dong-Kwon Rhee, Suhkneung Pyo
Toxicon.2013; 70: 142. CrossRef - Alveolar macrophages in pulmonary host defence – the unrecognized role of apoptosis as a mechanism of intracellular bacterial killing
J D Aberdein, J Cole, M A Bewley, H M Marriott, D H Dockrell
Clinical and Experimental Immunology.2013; 174(2): 193. CrossRef - The Role of ClpP in Protein Expression of Streptococcus pneumoniae
Qun Zhang, Yuanshuai Huang, Hong Wang, Wenchun Xu, Lan Liu, Yibing Yin, Xuemei Zhang
Current Microbiology.2012; 64(3): 294. CrossRef - Pneumococcal Gene Complex Involved in Resistance to Extracellular Oxidative Stress
Vahid Farshchi Andisi, Cecilia A. Hinojosa, Anne de Jong, Oscar P. Kuipers, Carlos J. Orihuela, Jetta J. E. Bijlsma, J. N. Weiser
Infection and Immunity.2012; 80(3): 1037. CrossRef - A proteome analysis of the response of a Pseudomonas aeruginosa oxyR mutant to iron limitation
Tiffany Vinckx, Qing Wei, Sandra Matthijs, Jean-Paul Noben, Ruth Daniels, Pierre Cornelis
BioMetals.2011; 24(3): 523. CrossRef
-
The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in
Streptococcus pneumoniae
- Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae
- Je Chul Lee , Dong Sun Kim , Dong Chan Moon , Jung-Hwa Lee , Mi Jin Kim , Su Man Lee , Yong Seok Lee , Se-Won Kang , Eun Jung Lee , Sang Sun Kang , Eunpyo Lee , Sung Hee Hyun
- J. Microbiol. 2009;47(5):641-645. Published online October 24, 2009
- DOI: https://doi.org/10.1007/s12275-009-0217-4
- 196 View
- 0 Download
- 15 Scopus
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Abstract
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- Nuclear targeting of bacterial proteins is an emerging pathogenic mechanism whereby bacterial proteins can interact with nuclear molecules and alter the physiology of host cells. The fully sequenced bacterial genome can predict proteins that target the nuclei of host cells based on the presence of nuclear localization signal (NLS). In the present study, we predicted bacterial proteins with the NLS sequences from Klebsiella pneumoniae by bioinformatic analysis, and 13 proteins were identified as carrying putative NLS sequences. Among them, HsdM, a subunit of KpnAI that is a type I restriction-modification system found in K. pneumoniae, was selected for the experimental proof of nuclear targeting in host cells. HsdM carried the NLS sequences, 7KKAKAKK13, in the N-terminus. A transient expression of HsdM-EGFP in COS-1 cells exhibited exclusively a nuclear localization of the fusion proteins, whereas the fusion proteins of HsdM with substitutions in residues lysine to alanine in the NLS sequences, 7AAAKAAA13, were localized in the cytoplasm. HsdM was co-localized with importin α in the nuclei of host cells. Recombinant HsdM alone methylated the eukaryotic DNA in vitro assay. Although HsdM tested in this study has not been considered to be a virulence factor, the prediction of NLS motifs from the full sequenced genome of bacteria extends our knowledge of functional genomics to understand subcellular targeting of bacterial proteins.
Journal Articles
- Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae
- Daiwen Xiao , Yongchang Yang , Hua Liu , Hua Yu , Yingjun Yan , Wenfang Huang , Wei Jiang , Weijin Liao , Qi Hu , Bo Huang
- J. Microbiol. 2009;47(5):646-650. Published online October 24, 2009
- DOI: https://doi.org/10.1007/s12275-009-0092-z
- 245 View
- 0 Download
- 2 Crossref
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Abstract
PDF
- A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.
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Citations
Citations to this article as recorded by- Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS
Daiwen Xiao, Yongchang Yang, Wei Jiang, Hangfeng Zhang, Hua Liu, Hua Yu, Chunbao Xie, Min Zhong, Liang Chen, Wenfang Huang
Journal of Microbiological Methods.2014; 105: 116. CrossRef - Advances in mass spectrometry for the identification of pathogens
Yen‐Peng Ho, P. Muralidhar Reddy
Mass Spectrometry Reviews.2011; 30(6): 1203. CrossRef
- Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS
- Proteomic Analysis of Protein Expression in Streptococcus pneumoniae in Response to Temperature Shift
- Myoung-Ro Lee , Song-Mee Bae , Tong-Soo Kim , Kwang-Jun Lee
- J. Microbiol. 2006;44(4):375-382.
- DOI: https://doi.org/2417 [pii]
- 209 View
- 0 Download
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Abstract
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- From its initial colonization to causation of disease, Streptococcus pneumoniae has evolved strategies to cope with a number of stressful in vivo environmental conditions. In order to analyze a global view of this organism’s response to heat shock, we established a 2-D electrophoresis proteome map of the S. pneumoniae D39 soluble proteins under in vitro culture conditions and performed the comparative proteome analysis to a 37 to 42°C temperature up-shift in S. pneumoniae. When the temperature of an exponentially growing S. pneumoniae D39 culture was raised to 42°C, the expression level of 25 proteins showed changes when compared to the control. Among these 25 proteins, 12 were identified by MALDI-TOF and LC-coupled ESI MS/MS. The identified proteins were shown to be involved in the general stress response, energy metabolism, nucleotide biosynthesis pathways, and purine metabolism. These results provide clues for understanding the mechanism of adaptation to heat shock by S. pneumoniae and may facilitate the assessment <br>of a possible role for these proteins in the physiology and pathogenesis of this pathogen.
Research Support, U.S. Gov't, Non-P.H.S.
- Utilization of Putrescine by Streptococcus pneumoniae During Growth in Choline-limited Medium
- D. Ware , J. Watt , E. Swiatlo
- J. Microbiol. 2005;43(5):398-405.
- DOI: https://doi.org/2284 [pii]
- 218 View
- 0 Download
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Abstract
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- Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in chemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.
Research Support, Non-U.S. Gov't
- The Effect of Transformation on the Virulence of Streptococcus pneumoniae
- Xue-Mei Zhang , Yi-Bing Yin , Dan Zhu , Bao-De Chen , Jin-Yong Luo , Yi-Ping Deng , Ming-Fang Liu , Shu-Hui Chen , Jiang-Ping Meng , Kai Lan , Yuan-Shuai Huang , Ge-Fei Kang
- J. Microbiol. 2005;43(4):337-344.
- DOI: https://doi.org/2256 [pii]
- 190 View
- 0 Download
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Abstract
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- Although pneumococcus is one of the most frequently encountered opportunistic pathogen in the world, the mechanisms responsible for its infectiveness have not yet been fully understood. In this paper, we have attempted to characterize the effects of pneumococcal transformation on the pathogenesis of the organism. We constructed three transformation-deficient pneumococcal strains, which were designated as Nos. 1d, 2d, and 22d. The construction of these altered strains was achieved via the insertion of the inactivated gene, comE, to strains 1, 2 and 22. We then conducted a comparison between the virulence of the transformation-deficient strains and that of the wild-type strains, via an evaluation of the ability of each strain to adhere to endothelial cells, and also assessed psaA mRNA expression, and the survival of hosts after bacterial challenge. Compared to what was observed with the wild-type strains, our results indicated that the ability of all of the transformation-deficient strains to adhere to the ECV304 cells had been significantly reduced (p < 0.05), the expression of psaA mRNA was reduced significantly (p < 0.05) in strains 2d and 22d, and the median survival time of mice infected with strains 1d and 2d was increased significantly after intraperitoneal bacterial challenge (p < 0.05). The results of our study also clearly indicated that transformation exerts significant effects on the virulence characteristics of S. pneumoniae, although the degree to which this effect is noted appears to depend primarily on the genetic background of the bacteria.
- Optimization of culture conditions for production of pneumococcal capsular polysaccharide type I
- Kim, Su Nam , Min, Kwan Ki , Kim, Seung Hwan , Choi, In Hwa , Lee, Suhk Hyung , Pyo, Suhk Noung , Rhee, Dong Kwon
- J. Microbiol. 1996;34(2):179-183.
- 207 View
- 2 Download
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Abstract
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- Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/l concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.
- Human Antibody Responses to Capsular Polysaccharides of Streptococcus pneumoniae 6B, 14, and 19F
- Kim, Ji Hye , Kim, Kyung Hyo , Kim, Jung Soo , Song, Jae Ho , Park, Moon Kook
- J. Microbiol. 1998;36(4):303-307.
- 243 View
- 0 Download
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Abstract
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- Human antibody responses to Streptococcus pneumoniae 6B, 14, and 19F capsular polysaccharide were analyzed. Thirty-one healthy young adults were immunized with the pneumococcal 23-valent PS vaccine. serum samples were obtained from them before and 1 month after vaccination. The amounts of total antibody, heavy chain and light chain isotypes were determined by enzyme-linked immunosorbent assay (ELISA). Vaccination increased the total lebvels of anti6B, anti-14, and anti-19F PS antibodies by 3.4-fold, 3.8-fold and 4.1-fold, respectively. Some inantibody was predominant in the responses to the three PSs, and most of the IgG anti-PS antibodies were IgG2 isotype. There was no significant difference in the k and λresponses.
Research Support, Non-U.S. Gov't
- Detection of Enterovirus, Cytomegalovirus, and Chlamydia pneumoniae in Atheromas
- Tae Won Kwon , Do Kyun Kim , Jeong Sook Ye , Won Joo Lee , Mi Sun Moon , Chul Hyun Joo , Heuiran Lee , Yoo Kyum Kim
- J. Microbiol. 2004;42(4):299-304.
- 181 View
- 0 Download
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Abstract
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- To investigate the presence of infectious agents in human atherosclerotic arterial tissues. Atherosclerotic plaques were removed from 128 patients undergoing carotid endarterectomy or other bypass procedures for occlusive disease, and from twenty normal arterial wall samples, obtained from transplant donors with no history of diabetes, hypertension, smoking, or hyperlipidemia. Using the polymerase chain reaction (PCR) or reverse transcription-PCR, these samples were analyzed for the presence of Chlamydia pneumoniae, cytomegalovirus, enterovirus, adenovirus, herpes simplex viruses types 1 and 2, and Epstein-Barr virus. The amplicons were then sequenced, and phylogenetic analyses were performed. Enteroviral RNA was found in 22 of 128 atherosclerotic vascular lesions (17.2%), and C. pneumoniae and cytomegalovirus were each found in 2 samples (1.6%). In contrast, adenovirus, herpes simplex viruses, and Epstein-Barr virus were not identified in any of the atherosclerotic samples. Enterovirus was detected in 6/24 (25.0%) aortas, 7/33 (21.2%) carotid arteries, 6/40 (15.0%) femoral arteries, and 3/31 (9.7%) radial arteries of patients with chronic renal failure. There were no infectious agents detected in any of the control specimens. Using phylogenetic analysis, the enterovirus isolates were clustered into 3 groups, arranged as echovirus 9 and coxsackieviruses B1 and B3. Enteroviral RNA was detected in 17.2% of atherosclerotic plaques, but was not observed in any of the control specimens. This suggests a connection between enteroviral infection and atherosclerosis. These findings differ from those of other studies, which found more frequent incidence of C. pneumoniae and cytomegalovirus infection in atherosclerotic plaques.
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