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HOME > J. Microbiol > Volume 49(3); 2011 > Article
Research Support, Non-U.S. Gov't
Predicting Genetic Traits and Epitope Analysis of apxIVA in Actinobacillus pleuropneumoniae
Min-Kyoung Shin , Seung-Bin Cha , Won-Jung Lee , Han Sang Yoo
Journal of Microbiology 2011;49(3):462-468.
DOI: https://doi.org/10.1007/s12275-011-0449-y
Published online: June 30, 2011
Department of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of KoreaDepartment of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Corresponding author:  Han Sang Yoo , Tel: +82-2-880-1263, 
Received: 3 November 2010   • Accepted: 13 January 2011
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Abstract

Actinobacillus pleuropneumoniae causes a severe hemorrhagic pneumonia in pigs. Fifteen serotypes of A. pleuropneumoniae express four different Apx toxins that belong to the pore-forming repeats-in-toxin (RTX) group of toxins. ApxIV, which is conserved and up-regulated in vivo, could be an excellent candidate for the development of a protective cross-serotype immunity vaccine, and could aid in the differential diagnosis of diseases caused by A. pleuropneumoniae. We identified and sequenced apxIVA from A. pleuropneumoniae serotype 2 isolated in Korea (Kor-ApxIVA). The Kor-ApxIVA was closely related to Switzerland (AF021919), China (CP000687), and China (GQ332268), showing 98.6%, 98.4%, and 97.2% amino acid homology, respectively. The level of amino acid homology, however, was higher than the nucleotide homology. The structural characteristics of ApxIVA showed RTX proteins, including N-terminal hydrophobic domains, signature sequences for potential acylation sites, and repeated glycine-rich nonapeptides in the C-terminal region of the protein. Thirty glycine-rich nonapeptides with the consensus sequence, L/V-X-G-G-X-G-N/D-D-X, were found in the C-terminus of the Kor-ApxIVA. In addition, the Kor-ApxIVA was predicted for the linear B-cell epitopes and conserved domains with determined peptide sequences. This genetic analysis of the Kor-ApxIVA might be an important foundation for future biological and functional research on ApxIVA.

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    Predicting Genetic Traits and Epitope Analysis of apxIVA in Actinobacillus pleuropneumoniae
    J. Microbiol. 2011;49(3):462-468.   Published online June 30, 2011
    DOI: https://doi.org/10.1007/s12275-011-0449-y
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