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HOME > J. Microbiol > Volume 49(5); 2011 > Article
Research Support, Non-U.S. Gov't
Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection
Li Jun Zhang 1, Li Jun Zhang 1, Wei Quan 1, Bei Bei Wang 1, Bing Ling Shen 1, Teng Teng Zhang 1, Yi Kang 2
Journal of Microbiology 2011;49(5):834-840.
DOI: https://doi.org/10.1007/s12275-011-1051-z
Published online: November 9, 2011
1Department of Pathophysiology, 2Department of Pharmacology, Basic Medical School of Tianjin Medical University, Tianjin 300070, P. R. China
Corresponding author:  Li Jun Zhang , Tel: +86-22-2354-2745, 
Received: 31 January 2011   • Accepted: 18 May 2011
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Abstract

This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.

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    Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection
    J. Microbiol. 2011;49(5):834-840.   Published online November 9, 2011
    DOI: https://doi.org/10.1007/s12275-011-1051-z
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