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HOME > J. Microbiol > Volume 56(8); 2018 > Article
Journal Article
Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
Min Yong Jwa 1, Soyoung Jeong 1, Eun Byeol Ko 1, A Reum Kim 1, Hyun Young Kim 1, Sun Kyung Kim 1, Ho Seong Seo 2, Cheol-Heui Yun 3, Seung Hyun Han 1
Journal of Microbiology 2018;56(8):579-585.
DOI: https://doi.org/10.1007/s12275-018-8347-1
Published online: July 25, 2018
1Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, 2Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea
Corresponding author:  Seung Hyun Han , Tel: +82-2-880-2310;, 
Received: 26 June 2018   • Revised: 2 July 2018   • Accepted: 2 July 2018
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Abstract

Streptococcus pneumoniae is a major respiratory pathogen that causes millions of deaths worldwide. Although subunit vaccines formulated with the capsular polysaccharides or their protein conjugates are currently-available, low-cost vaccines with wide serotype coverage still remain to be developed, especially for developing countries. Recently, gamma- irradiation has been considered as an effective inactivation
method
to prepare S. pneumoniae vaccine candidate. In this study, we investigated the immunogenicity and protective immunity of gamma-irradiated S. pneumoniae (r-SP), by comparing with heat-inactivated S. pneumoniae (h-SP) and formalin-inactivated S. pneumoniae (f-SP), both of which were made by traditional inactivation methods. Intranasal immunization of C57BL/6 mice with r-SP in combination with cholera toxin as an adjuvant enhanced S. pneumoniaespecific antibodies on the airway mucosal surface and in sera more potently than that with h-SP or f-SP under the same conditions. In addition, sera from mice immunized with r- SP potently induced opsonophagocytic killing activity more effectively than those of h-SP or f-SP, implying that r-SP could induce protective antibodies. Above all, immunization with r-SP effectively protected mice against S. pneumoniae infection. Collectively, these results suggest that gamma- irradiation is an effective method for the development of a killed whole cell pneumococcal vaccine that elicits robust mucosal and systemic immune responses.

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    Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
    J. Microbiol. 2018;56(8):579-585.   Published online July 25, 2018
    DOI: https://doi.org/10.1007/s12275-018-8347-1
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