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Editor's Choice 2024

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Editor’s Choice articles are curated by our senior editors, who represent each section, to highlight research published in 2024 that they consider particularly interesting to our readers and/or important within the respective research area.

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Microbial Physiology and Biochemistry
Journal Articles
Mycobacterium tuberculosis PE_PGRS45 (Rv2615c) Promotes Recombinant Mycobacteria Intracellular Survival via Regulation of Innate Immunity, and Inhibition of Cell Apoptosis
Tao Xu , Chutong Wang , Minying Li , Jing Wei , Zixuan He , Zhongqing Qian , Xiaojing Wang , Hongtao Wang
J. Microbiol. 2024;62(1):49-62.   Published online February 9, 2024
DOI: https://doi.org/10.1007/s12275-023-00101-0
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  • 4 Web of Science
  • 4 Crossref
AbstractAbstract PDF
Tuberculosis (TB), a bacterial infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis), is a significant global public health problem. Mycobacterium tuberculosis expresses a unique family of PE_PGRS proteins that have been implicated in pathogenesis. Despite numerous studies, the functions of most PE_PGRS proteins in the pathogenesis of mycobacterium infections remain unclear. PE_PGRS45 (Rv2615c) is only found in pathogenic mycobacteria. In this study, we successfully constructed a recombinant Mycobacterium smegmatis (M. smegmatis) strain which heterologously expresses the PE_PGRS45 protein. We found that overexpression of this cell wall-associated protein enhanced bacterial viability under stress in vitro and cell survival in macrophages. MS_PE_PGRS45 decreased the secretion of pro-inflammatory cytokines such as IL-1β, IL-6, IL-12p40, and TNF-α. We also found that MS_PE_PGRS45 increased the expression of the anti-inflammatory cytokine IL-10 and altered macrophage-mediated immune responses. Furthermore, PE_PGRS45 enhanced the survival rate of M. smegmatis in macrophages by inhibiting cell apoptosis. Collectively, our findings show that PE_PGRS45 is a virulent factor actively involved in the interaction with the host macrophage.

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Citations to this article as recorded by  
  • Evolution of the PE_PGRS Proteins of Mycobacteria: Are All Equal or Are Some More Equal than Others?
    Bei Chen, Belmin Bajramović, Bastienne Vriesendorp, Herman Pieter Spaink
    Biology.2025; 14(3): 247.     CrossRef
  • Recent advances in research on Mycobacterium tuberculosis virulence factors and their role in pathogenesis
    Ming-Rui Sun, Jia-Yin Xing, Xiao-Tian Li, Ren Fang, Yang Zhang, Zhao-Li Li, Ning-Ning Song
    Journal of Microbiology, Immunology and Infection.2025; 58(5): 497.     CrossRef
  • Rv2741 Promotes Mycobacterium Survival by Modulating Macrophage Function via the IL-1α-MAPK Axis
    Xintong He, Yonglin He, Xichuan Deng, Nan Lu, Anlong Li, Sijia Gao, Shiyan He, Yuran Wang, Nanzhe Fu, Zijie Wang, Yuxin Nie, Lei Xu
    ACS Infectious Diseases.2025; 11(3): 676.     CrossRef
  • The PE/PPE family proteins of Mycobacterium tuberculosis: evolution, function, and prospects for tuberculosis control
    Zhijing Zhang, Le Dong, Xin Li, Taibing Deng, Qinglan Wang
    Frontiers in Immunology.2025;[Epub]     CrossRef
RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA
Uri Han, Nara Han, Byeonggyu Park, Taeck Joong Jeon
J. Microbiol. 2024;62(8):627-637.   Published online June 17, 2024
DOI: https://doi.org/10.1007/s12275-024-00143-y
  • 442 View
  • 7 Download
  • 2 Web of Science
  • 2 Crossref
AbstractAbstract PDF
Ras small GTPases act as molecular switches in various cellular signaling pathways, including cell migration, proliferation, and differentiation. Three Rap proteins are present in Dictyostelium; RapA, RapB, and RapC. RapA and RapC have been reported to have opposing functions in the control of cell adhesion and migration. Here, we investigated the role of RapB, a member of the Ras GTPase subfamily in Dictyostelium, focusing on its involvement in cell adhesion, migration, and developmental processes. This study revealed that RapB, similar to RapA, played a crucial role in regulating cell morphology, adhesion, and migration. rapB null cells, which were generated by CRISPR/Cas9 gene editing, displayed altered cell size, reduced cell-substrate adhesion, and increased migration speed during chemotaxis. These phenotypes of rapB null cells were restored by the expression of RapB and RapA, but not RapC. Consistent with these results, RapB, similar to RapA, failed to rescue the phenotypes of rapC null cells, spread morphology, increased cell adhesion, and decreased migration speed during chemotaxis. Multicellular development of rapB null cells remained unaffected. These results suggest that RapB is involved in controlling cell morphology and cell adhesion. Importantly, RapB appears to play an inhibitory role in regulating the migration speed during chemotaxis, possibly by controlling cell-substrate adhesion, resembling the functions of RapA. These findings contribute to the understanding of the functional relationships among Ras subfamily proteins.

Citations

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  • Intracellular Calcium Responses to External Calcium Stimuli in Dictyostelium
    Dahyeon Kim, Jiseong Seo, Taeck Joong Jeon
    Journal of Microbiology and Biotechnology.2025;[Epub]     CrossRef
  • Advanced MD Simulation Methods Uncover Mechanisms of SH3 Domain Functions in Small GTPase Signaling
    Muslum Yildiz
    Proteins: Structure, Function, and Bioinformatics.2025; 93(12): 2055.     CrossRef
Unexpected Requirement of Small Amino Acids at Position 183 for DNA Binding in the Escherichia coli cAMP Receptor Protein
Marcus Carranza, Amanda Rea, Daisy Pacheco, Christian Montiel, Jin Park, Hwan Youn
J. Microbiol. 2024;62(10):871-882.   Published online September 6, 2024
DOI: https://doi.org/10.1007/s12275-024-00169-2
  • 456 View
  • 9 Download
  • 2 Web of Science
  • 1 Crossref
AbstractAbstract PDF
The Escherichia coli cAMP receptor protein (CRP) relies on the F-helix, the recognition helix of the helix-turn-helix motif, for DNA binding. The importance of the CRP F-helix in DNA binding is well-established, yet there is little information on the roles of its non-base-contacting residues. Here, we show that a CRP F-helix position occupied by a non-base-contacting residue Val183 bears an unexpected importance in DNA binding. Codon randomization and successive in vivo screening selected six amino acids (alanine, cysteine, glycine, serine, threonine, and valine) at CRP position 183 to be compatible with DNA binding. These amino acids are quite different in their amino acid properties (polar, non-polar, hydrophobicity), but one commonality is that they are all relatively small. Larger amino acid substitutions such as histidine, methionine, and tyrosine were made site-directedly and showed to have no detectable DNA binding, further supporting the requirement of small amino acids at CRP position 183. Bioinformatics analysis revealed that small amino acids (92.15% valine and 7.75% alanine) exclusively occupy the position analogous to CRP Val183 in 1,007 core CRP homologs, consistent with our mutant data. However, in extended CRP homologs comprising 3700 proteins, larger amino acids could also occupy the position analogous to CRP Val183 albeit with low occurrence. Another bioinformatics analysis suggested that large amino acids could be tolerated by compensatory small-sized amino acids at their neighboring positions. A full understanding of the unexpected requirement of small amino acids at CRP position 183 for DNA binding entails the verification of the hypothesized compensatory change(s) in CRP.

Citations

Citations to this article as recorded by  
  • SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence in Streptococcus pneumoniae D39
    Ye Tao, Li Lei, Shuhui Wang, Xuemei Zhang, Yibing Yin, Yuqiang Zheng
    Frontiers in Microbiology.2025;[Epub]     CrossRef

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