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HOME > J. Microbiol > Volume 47(1); 2009 > Article
Randomized Controlled Trial
Antimicrobial Activity of Enterocins from Enterococcus faecalis SL-5 against Propionibacterium acnes, the Causative Agent in Acne Vulgaris, and Its Therapeutic Effect
Bong Seon Kang 1, Jae-Gu Seo 2, Gwa-Su Lee 2, Jung-Hwa Kim 2, Sei Yeon Kim 1, Ye Won Han 1, Hoon Kang 3, Hyung Ok Kim 1, Ji Hwan Rhee 2, Myung-Jun Chung 2, Young Min Park 1
Journal of Microbiology 2009;47(1):101-109.
DOI: https://doi.org/10.1007/s12275-008-0179-y
Published online: February 20, 2009
1Department of Dermatology, Kangnam St. Mary’s Hospital, The Catholic University of Korea, Seoul 137-040, Republic of Korea, 2R&D Center, Cellbiotech, Co. Ltd., Gimpo 415-871, Republic of Korea, 3St. Paul’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Republic of Korea
Corresponding author:  Young Min Park , Tel: 82-2-590-1351, 
Received: 15 July 2008   • Accepted: 10 October 2008
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Abstract

A lactic acid bacterial strain was isolated from human fecal specimen and identified as Enterococcus faecalis SL-5. The isolated strain showed antimicrobial activity against Gram-positive pathogens assayed, especially the highest activity against Propionibacterium acnes. The antimicrobial substance was purified and verified as a bacteriocin (named ESL5) of E. faecalis SL-5 by activity-staining using P. acnes as an indicator. N-terminal sequence of ESL5 was determined (MGAIAKLVAK) and sequence analysis revealed that it is almost identical to the some of enterocins including L50A/B of E. faecium L50 and MR10A/B of E. faecalis MRR 10-3. From the sequencing data of L50A/B structural genes, the nucleotide sequence showed 100% identity with that of the MR10A/B structural genes, implying that ESL5 is an equivalent of enterocin MR10. Meanwhile, we also tested the therapeutic effect of anti-P. acnes activity in patients with mild to moderate acne because of its pathogenic role to acne vulgaris. For this purpose, a concentrated powder of CBT SL-5 was prepared using cell-free culture supernatant (CFCS) of E. faecalis SL-5 and included in a lotion for application in the patients. The study showed that CBT SL-5 lotion significantly reduced the inflammatory lesions like pustules compared to the placebo lotion. Therefore our results indicate that the anti-P. acnes activity produced by E. faecalis SL-5 has potential role to the treatment of acne as an alternative to topical antibiotics.

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    Antimicrobial Activity of Enterocins from Enterococcus faecalis SL-5 against Propionibacterium acnes, the Causative Agent in Acne Vulgaris, and Its Therapeutic Effect
    J. Microbiol. 2009;47(1):101-109.   Published online February 20, 2009
    DOI: https://doi.org/10.1007/s12275-008-0179-y
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