Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India

Joseph J. Kingston; Kuruvilla Zachariah; Urmil Tuteja; Sanjay Kumar; Harsh Vardhan Batra

doi:10.1007/s12275-008-0162-7

Articles

Page Path: HOME > J. Microbiol > Volume 47(1); 2009 > Article

Journal Article
Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India: Joseph J. Kingston ¹, Kuruvilla Zachariah ¹, Urmil Tuteja ², Sanjay Kumar ², Harsh Vardhan Batra ¹; Journal of Microbiology 2009;47(1):110-115.
DOI: https://doi.org/10.1007/s12275-008-0162-7
Published online: February 20, 2009

¹Division of Microbiology, Defence Food Research Laboratory, Sidhartha Nagar, Mysore, Karnataka 570011, India, ²Division of Microbiology, Defence R&D Establishment, Jhansi Road, Gwalior, Madhya Pradesh 474002, India

Corresponding author: Harsh Vardhan Batra , Tel: 91-821-247-9779,

Received: 1 July 2008 • Accepted: 10 November 2008

prev next

77 Views
0 Download
6 Crossref
12 Scopus

Full Article

Abstract

Vibrio cholerae isolates recovered from cholera outbreaks in Bhind district of Madhya Pradesh and Delhi, Northern India were characterized. The O1 serogroup isolates from Bhind outbreak were of Inaba serotype whereas both Ogawa and Inaba serotypes were recovered from Delhi. PCR analysis revealed that only O1 serogroup V. cholerae isolates carried the virulence-associated genes like ctxA, tcpA, ace, and zot. Molecular typing by repetitive sequence based ERIC, VCR1, and VC1 PCR’s revealed similar DNA profile for both Inaba and Ogawa serotypes. A discrete VC1-PCR band identified among the El Tor strains had greater similarity (>97%) to the V. cholerae genome sequence and therefore has the potential to be used as a marker for the identification of the V. cholerae strains. Non-O1 strains recovered from Bhind region differed among themselves as well as from that of the O1 isolates. All the O1 serogroup isolates possessed SXT element and were uniformly resistant to the antibiotics nalidixic acid, polymyxin-B, furazolidone, cloxacilin, trimethoprim-sulfamethaxazole, and vibriostatic agent O129. Inaba strains from both Delhi and Bhind differed from Ogawa strains by their resistance to streptomycin despite sharing similar DNA patterns in all the three rep-PCRs. Though Delhi and Bhind are separate geographical regions in Northern India, Inaba strains from both these places appear to be closely related owing to their similarity in antibiogram and genetic profile.

Keywords: ERIC-PCR;DNA fingerprinting;SXT;VC1-PCR;Vibrio cholerae

Figure & Data

References

Citations

Citations to this article as recorded by

Clonal relationship among Vibrio parahaemolyticus isolated from Mediterranean mussels (Mytilus galloprovincialis) and grooved carpet shells (Ruditapes decussatus) harvested in Sardinia (Italy)
Sonia Lamon, Asmine Bastardo, Domenico Meloni, Simonetta Gianna Consolati, Federica Fois, Gabriella Porcheddu, Vanessa Agus, Margherita Pes, Maria Giovanna Cambula, Anna Mureddu, Jesus L. Romalde
Food Microbiology.2019; 84: 103258. CrossRef
Virulence gene profiles, biofilm formation, and antimicrobial resistance of Vibrio cholerae non-O1/non-O139 bacteria isolated from West Bengal, India
Parimal Dua, Amit Karmakar, Chandradipa Ghosh
Heliyon.2018; 4(12): e01040. CrossRef
Virulence and Multidrug Resistance Patterns ofVibrio choleraeO1 Isolates from Diarrheal Outbreaks of South India During 2006–2009
Kannan Balaji, Peter Awili Okonjo, Ramalingam Thenmozhi, Shunmugiah Karutha Pandian
Microbial Drug Resistance.2013; 19(3): 198. CrossRef
The use of multiple typing methods allows a more accurate molecular characterization ofVibrio parahaemolyticusstrains isolated from the Italian Adriatic Sea
Greta Caburlotto, Maria M. Lleo, Micol Gennari, Sabela Balboa, Jesus L. Romalde
FEMS Microbiology Ecology.2011; 77(3): 611. CrossRef
Continuing challenge of infectious diseases in India
T Jacob John, Lalit Dandona, Vinod P Sharma, Manish Kakkar
The Lancet.2011; 377(9761): 252. CrossRef
Chemoprophylaxis in Contacts of Patients with Cholera: Systematic Review and Meta-Analysis
Ludovic Reveiz, Evelina Chapman, Pilar Ramon-Pardo, Tracey Perez Koehlmoos, Luis Gabriel Cuervo, Sylvain Aldighieri, Amy Chambliss, Lorenz von Seidlein
PLoS ONE.2011; 6(11): e27060. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India

J. Microbiol. 2009;47(1):110-115. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0162-7

Related articles