Alpha‑Hemolysin from Staphylococcus aureus Obstructs Yeast‑Hyphae Switching and Diminishes Pathogenicity in Candida albicans

Xiaoyu Yu; Yinhe Mao; Guangbo Li; Xianwei Wu; Qiankun Xuan; Simin Yang; Xiaoqing Chen; Qi Cao; Jian Guo; Jinhu Guo; Wenjuan Wu

doi:10.1007/s12275-022-00006-4

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Alpha‑Hemolysin from Staphylococcus aureus Obstructs Yeast‑Hyphae Switching and Diminishes Pathogenicity in Candida albicans: Xiaoyu Yu ¹, Yinhe Mao ², Guangbo Li ¹, Xianwei Wu ², Qiankun Xuan ¹, Simin Yang ¹, Xiaoqing Chen ², Qi Cao ³, Jian Guo ¹, Jinhu Guo ⁴, Wenjuan Wu ¹; Journal of Microbiology 2023;61(2):233-243.
DOI: https://doi.org/10.1007/s12275-022-00006-4
Published online: February 9, 2023

¹Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, People’s Republic of China, ²Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, People’s Republic of China, ³Pharmaceutical Analysis Center, School of Pharmacy, The Naval Military Medical University, Shanghai 200433, People’s Republic of China, ⁴Department of Clinical Laboratory, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People’s Republic of China¹Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, People’s Republic of China, ²Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, People’s Republic of China, ³Pharmaceutical Analysis Center, School of Pharmacy, The Naval Military Medical University, Shanghai 200433, People’s Republic of China, ⁴Department of Clinical Laboratory, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People’s Republic of China

Corresponding author: Xiaoyu Yu ,
Wenjuan Wu ,

Received: 9 June 2022 • Revised: 18 November 2022 • Accepted: 29 November 2022

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Abstract

The use of antibiotics can disrupt the body’s natural balance and increase the susteptibility of patients towards fungal infections. Candida albicans is a dimorphic opportunistic fungal pathogen with niches similar to those of bacteria. Our aim was to study the interaction between this pathogen and bacteria to facilitate the control of C. albicans infection. Alpha-hemolysin (Hla), a protein secreted from Staphylococcus aureus, causes cell wall damage and impedes the yeast–hyphae transition in C. albicans. Mechanistically, Hla stimulation triggered the formation of reactive oxygen species that damaged the cell wall and mitochondria of C. albicans. The cell cycle was arrested in the G0/G1 phase, CDC42 was downregulated, and Ywp1 was upregulated, disrupting yeast hyphae switching. Subsequently, hyphae development was inhibited. In mouse models, C. albicans pretreated with Hla reduced the C. albicans burden in skin and vaginal mucosal infections, suggesting that S. aureus Hla can inhibit hyphal development and reduce the pathogenicity of candidiasis in vivo.

Keywords: FtsH;LapB;LpxC;YejM;cold stress;lipopolysaccharide;protease;protein-protein interaction

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Candida albicans and Candida glabrata : global priority pathogens
Myrto Katsipoulaki, Mark H. T. Stappers, Dhara Malavia-Jones, Sascha Brunke, Bernhard Hube, Neil A. R. Gow, Joseph Heitman
Microbiology and Molecular Biology Reviews.2024;[Epub] CrossRef

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Alpha‑Hemolysin from Staphylococcus aureus Obstructs Yeast‑Hyphae Switching and Diminishes Pathogenicity in Candida albicans

J. Microbiol. 2023;61(2):233-243. Published online February 9, 2023

DOI: https://doi.org/10.1007/s12275-022-00006-4

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