Poly-γ-glutamic acid productivity of Bacillus subtilis BsE1 has positive function in motility and biocontrol against Fusarium graminearum

Luyao Wang; Ning Wang; Dandan Mi; Yuming Luo; Jianhua Guo

doi:10.1007/s12275-017-6589-y

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Poly-γ-glutamic acid productivity of Bacillus subtilis BsE1 has positive function in motility and biocontrol against Fusarium graminearum: Luyao Wang ¹, Ning Wang ¹, Dandan Mi ¹, Yuming Luo ², Jianhua Guo ¹; Journal of Microbiology 2017;55(7):554-560.
DOI: https://doi.org/10.1007/s12275-017-6589-y
Published online: June 30, 2017

¹Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education; Nanjing 210095, P. R. China, ²Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Science, Huaiyin Normal University, Huaian 223300, P. R. China¹Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education; Nanjing 210095, P. R. China, ²Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Science, Huaiyin Normal University, Huaian 223300, P. R. China

Corresponding author: Yuming Luo , Tel: +86-25-84395312,
Jianhua Guo , Tel: +86-25-84395312,

Received: 18 November 2016 • Revised: 5 April 2017 • Accepted: 18 April 2017

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Abstract

In this study, we investigate the relationship between γ-PGA productivity and biocontrol capacity of Bacillus subtilis BsE1; one bacterial isolate displayed 62.14% biocontrol efficacy against Fusarium root rot. The γ-PGA yield assay, motility assay, wheat root colonization assay, and biological control assay were analysed in different γ-PGA yield mutants of BsE1. The pgsB (PGA-synthase-CapB gene) deleted mutant of BsE1 reduced γ-PGA yield and exhibited apparent decline of in vitro motile ability. Deletion of pgsB impaired colonizing capacity of BsE1 on wheat root in 30 days, also lowered biocontrol efficacies from 62.08% (wild type BsE1) to 14.22% in greenhouse experiment against Fusarium root rot. The knockout of pgdS and ggt (genes relate to two γ-PGA degrading enzymes) on BsE1, leads to a considerable improvement in polymer yield and biocontrol efficacy, which attains higher level compared with wild type BsE1. Compared with ΔpgsB mutant, defense genes related to reactive oxygen species (ROS) and phytoalexin expressed changes by notable levels on wheat roots treated with BsE1, demonstrating the functional role γ-PGA plays in biocontrol against Fusarium root rot. γ-PGA is not only important to the motile and plant root colonization ability of BsE1, but also essential to the biological control performed by BsE1 against Fusarium root rot. Our goal in this study is to reveals a new perspective of BCAs screening on bacterial isolates, without good performance during pre-assays of antagonism ability.

Keywords: biocontrol;poly-γ-glutamic acid;motility;root colonization;Fusarium root rot

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Poly-γ-glutamic acid productivity of Bacillus subtilis BsE1 has positive function in motility and biocontrol against Fusarium graminearum

J. Microbiol. 2017;55(7):554-560. Published online June 30, 2017

DOI: https://doi.org/10.1007/s12275-017-6589-y

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