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HOME > J. Microbiol > Volume 55(7); 2017 > Article
Journal Article
Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach
Bangzheng Wang 1, Tao Niu 1, Muhammad Zeeshan Bhatti 2, Fenfen Chen 1, Lin Wu 1, Jiwu Chen 1
Journal of Microbiology 2017;55(7):545-553.
DOI: https://doi.org/10.1007/s12275-017-6422-7
Published online: June 30, 2017
1School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China, 2Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China1School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China, 2Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
Corresponding author:  Jiwu Chen , Tel: +86-021-24206741, 
Received: 26 August 2016   • Revised: 2 January 2017   • Accepted: 13 March 2017
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Abstract

Euplotes encysticus is a species of Hypotrich ciliates, which form cyst wall by secreting the special substances on encounter of adverse environment. It has critical significance to study the component and mechanism underlying resting cyst, during resisting unfavorable conditions in dormancy induction. The present study was aimed to investigate the effects of cyst wall proteins of Euplotes encysticus by using biochemical
methods
. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools. We detected 42 cyst wall proteins, 26 were functional proteins and 16 proteins consist of unknown function; which is consistent with cyst wall specificity. These results partially revealed the components of resting cyst wall formed after the cells differentiation of Euplotes encysticus. In addition, our data suggested that the function of cyst wall proteins are more likely involved in the mechanical protection, signal transduction, material transport, protein degradation and energy metabolism to survival, with potentially importance implications in the molecular mechanism of eukaryocyte dormancy under stress condition.

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    Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach
    J. Microbiol. 2017;55(7):545-553.   Published online June 30, 2017
    DOI: https://doi.org/10.1007/s12275-017-6422-7
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