Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans

Won-Jae Chi; Soon-Youl Lee; JaeHag Lee

doi:10.1007/s12275-011-1100-7

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Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans: Won-Jae Chi ¹, Soon-Youl Lee ², JaeHag Lee ³; Journal of Microbiology 2011;49(5):828-833.
DOI: https://doi.org/10.1007/s12275-011-1100-7
Published online: November 9, 2011

¹Department of Biological Science, Myongji University, Gyeonggi-do 449-728, Republic of Korea, ²Department of Biotechnology, Hankyong National University, Gyeonggi-do 456-749, Republic of Korea, ³Department of Food and Nutrition, Seoil University, Seoul 131-702, Republic of Korea¹Department of Biological Science, Myongji University, Gyeonggi-do 449-728, Republic of Korea, ²Department of Biotechnology, Hankyong National University, Gyeonggi-do 456-749, Republic of Korea, ³Department of Food and Nutrition, Seoil University, Seoul 131-702, Republic of Korea

Corresponding author: JaeHag Lee , Tel: +82-2-490-7509,

Received: 2 March 2011 • Accepted: 16 May 2011

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Abstract

The Gram-positive mycelium-producing bacterium Streptomyces undergoes complex morphological differentiation after autolytic degradation of the vegetative mycelium. Cell-wall breakdown during growth stimulates cell development and secondary metabolite production by Streptomyces. N-acetylglucosamine (GlcNAc) produced by cell-wall lysis acts as a signal molecule, triggering the production of secondary metabolites in S. coelicolor A3(2). Here, we report that introduction of multiple copies of the GlcNAc-internalizing gene (sgr4635, encoding nagE2) of S. griseus activates actinorhodin and undecylprodigiosin production during the late growth of S. lividans in the absence of GlcNAc. Furthermore, the repressor-type transcriptional regulator DasR binds to two operator sites upstream of sgr4635. Our findings indicate that sgr4635 induces DasR-mediated antibiotic production by internalizing the GlcNAc accumulated from cell-wall lysis.

Keywords: Streptomyces;antibiotic production;N-acetylglucosamine;transcriptional regulator;DasR

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Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans

J. Microbiol. 2011;49(5):828-833. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1100-7

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