Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid

Xia Wan; Yinbo Zhang; Ping Wang; Mulan Jiang

doi:10.1007/s12275-011-0254-7

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Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid: Xia Wan ^1,2, Yinbo Zhang ¹, Ping Wang ¹, Mulan Jiang ^1,2; Journal of Microbiology 2011;49(1):151-154.
DOI: https://doi.org/10.1007/s12275-011-0254-7
Published online: March 3, 2011

¹Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China, ²Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wuhan 430062, P. R. China

Corresponding author: Mulan Jiang , Tel: +86-27-8683-8791,

Received: 8 July 2010 • Accepted: 5 November 2010

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Abstract

The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ6,9,12) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.

Keywords: desaturase;Rhizopus stolonifer;gamma-linolenic acid

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Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid

J. Microbiol. 2011;49(1):151-154. Published online March 3, 2011

DOI: https://doi.org/10.1007/s12275-011-0254-7

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