Evaluation of Three Molecular Methods of Repetitive Element Loci for Differentiation of Mycobacterium avium subsp. paratuberculosis (MAP)

Amr El-Sayed; Abdulwahed Ahmed Hassan; Saleh Natour; Amir Abdulmawjood; Michael Bulte; Wilfried Wolter; Michael Zschock

doi:10.1007/s12275-008-0257-1

Articles

Page Path: HOME > J. Microbiol > Volume 47(3); 2009 > Article

Research Support, Non-U.S. Gov't
Evaluation of Three Molecular Methods of Repetitive Element Loci for Differentiation of Mycobacterium avium subsp. paratuberculosis (MAP): Amr El-Sayed ^1,2, Abdulwahed Ahmed Hassan ^1,3, Saleh Natour ¹, Amir Abdulmawjood ⁴, Michael Bulte ⁴, Wilfried Wolter ⁵, Michael Zschock ¹; Journal of Microbiology 2009;47(3):253-259.
DOI: https://doi.org/10.1007/s12275-008-0257-1
Published online: June 26, 2009

¹Landesbetrieb Hessisches Landeslabor, Schubertstrasse 60, Haus 13. D-35392, Giessen, Germany, ²Institute of Internal Medicine and Animal Infectious Disease, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Cairo, Egypt, ³GD Animal Health Service, Department of Bacteriology and Parasitology, Postbus 9, 7400 AA Deventer, The Netherlands, ⁴Institut f? Tier?ztliche Nahrungsmittelkunde, Justus-Liebig-Universit?, Frankfurterstr. 92, Giessen, Germany, ⁵Regierungspr?idum Giessen, Dez. 51.2, Schanzenfeldstrasse 8, 35578 Wetzlar, Germany¹Landesbetrieb Hessisches Landeslabor, Schubertstrasse 60, Haus 13. D-35392, Giessen, Germany, ²Institute of Internal Medicine and Animal Infectious Disease, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Cairo, Egypt, ³GD Animal Health Service, Department of Bacteriology and Parasitology, Postbus 9, 7400 AA Deventer, The Netherlands, ⁴Institut f? Tier?ztliche Nahrungsmittelkunde, Justus-Liebig-Universit?, Frankfurterstr. 92, Giessen, Germany, ⁵Regierungspr?idum Giessen, Dez. 51.2, Schanzenfeldstrasse 8, 35578 Wetzlar, Germany

Corresponding author: Abdulwahed Ahmed Hassan , Tel: 31-570-66-0640,

Received: 28 October 2008 • Accepted: 4 February 2009

prev next

57 Views
0 Download
13 Crossref
15 Scopus

Full Article

Abstract

The aim of the present study is to evaluate the efficiency of three methods to determine the molecular diversity of 34 Mycobacterium avium subsp. paratuberculosis (MAP) strains isolated from 17 cattle herds. The applied methods included the analysis of sequence polymorphism of the mononucleotide (G1 and G2) and trinucleotide sequences (GGT) of the Short Sequence Repeats (SSR) and the determination of size polymorphism of 9 different Mycobacterial Interspersed Repetitive Units (MIRU) and 6 Variable Number Tandem Repeats (VNTR). Sequence analysis of SSR of 34 isolates showed 4, 6, and 2 alleles of G1, G2, and GGT repeats, respectively. The amplification of the investigated 9 MIRU units revealed only two discriminatory genotyping systems (MIRU2 and MIRU3). Out of 6 VNTR PCR differentiation methods, only one method could be recommended for genotyping purposes. The profile 7g-12g-4ggt-II-b-2 of the combination systems G1-G2-GGT-MIRU2-MIRU3-VNTR1658 dominates among the examined isolates and was
detected in 14.7% of the isolates. The use of certain repetitive loci of SSR, MIRU, and VNTR techniques in this study showed greater potential than others for the characterization of MAP isolates. The recommended loci can be used for the epidemiological tracing of MAP field strains and to determine the relationships
between isolates in different herds.

Keywords: Mycobacterium avium subsp. paratuberculosis (MAP);short sequence repeats (SSR);mycobacterial interspersed repetitive units (MIRU);variable number tandem repeats (VNTR);Johne's disease (JD)

Figure & Data

References

Citations

Citations to this article as recorded by

More insights about genomic population structure of Mycobacterium avium subspecies paratuberculosis (Map) from multiple hosts in west and central provinces of Iran using a boosted genotyping approach
Reza Najafpour, Mohammad Reza Zolfaghari, Nader Mosavari, Razieh Nazari, Keyvan Tadayon
Comparative Immunology, Microbiology and Infectious Diseases.2023; 100: 101912. CrossRef
Genotyping methods and molecular epidemiology ofMycobacterium aviumsubsp.paratuberculosis(MAP)
Ahmad Fawzy, Michael Zschöck, Christa Ewers, Tobias Eisenberg
International Journal of Veterinary Science and Medicine.2018; 6(2): 258. CrossRef
Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis
Milka P. Podder, Susan E. Banfield, Greg P. Keefe, Hugh G. Whitney, Kapil Tahlan, Igor Mokrousov
PLOS ONE.2015; 10(4): e0126071. CrossRef
Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods
G. S. Abdellrazeq, M. M. El-Naggar, S. A. Khaliel, A. E. Gamal-Eldin
Veterinary World.2014; 7(8): 586. CrossRef
Molecular characterization ofMycobacterium aviumsubsp.paratuberculosisfield isolates recovered from dairy cattle in Germany
Mohamed Salem, Saleh Natur, Amr A. El-Sayed, Abdulwahed Hassan, Georg Baljer, Michael Zschöck
International Journal of Veterinary Science and Medicine.2013; 1(1): 30. CrossRef
Mycobacterium avium subspecies paratuberculosis: an insidious problem for the ruminant industry
Mohamed Salem, Carsten Heydel, Amr El-Sayed, Samia A. Ahmed, Michael Zschöck, George Baljer
Tropical Animal Health and Production.2013; 45(2): 351. CrossRef
Genotyping ofMycobacterium aviumfield isolates based on repetitive elements
A. El-Sayed, S. Natur, Nadra-Elwgoud M.I. Abdou, M. Salem, A. Hassan, M. Zschöck
International Journal of Veterinary Science and Medicine.2013; 1(1): 36. CrossRef
Progress in molecular typing of Mycobacterium avium subspecies paratuberculosis
Elena Castellanos, Lucía de Juan, Lucas Domínguez, Alicia Aranaz
Research in Veterinary Science.2012; 92(2): 169. CrossRef
Isolation of Mycobacterium avium subspecies paratuberculosis from Ugandan cattle and strain differentiation using optimised DNA typing techniques
Julius Okuni, Chrysostomos I Dovas, Panayiotis Loukopoulos, Ilias G Bouzalas, David Kateete, Moses L Joloba, Lonzy Ojok
BMC Veterinary Research.2012; 8(1): 99. CrossRef
Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis at a regional scale in Germany
J.A. Fernández-Silva, A. Abdulmawjood, Ö. Akineden, K. Dräger, W. Klawonn, M. Bülte
Research in Veterinary Science.2012; 93(2): 776. CrossRef
Suspicion of Mycobacterium avium subsp. paratuberculosis Transmission between Cattle and Wild-Living Red Deer (Cervus elaphus) by Multitarget Genotyping
Isabel Fritsch, Gabriele Luyven, Heike Köhler, Walburga Lutz, Petra Möbius
Applied and Environmental Microbiology.2012; 78(4): 1132. CrossRef
Effectiveness of combination of Mini-and Microsatellite loci to sub-type Mycobacterium avium subsp. paratuberculosis Italian type C isolates
Matteo Ricchi, Gianluca Barbieri, Roberta Taddei, Gian L Belletti, Elena Carra, Giuliana Cammi, Chiara A Garbarino, Norma Arrigoni
BMC Veterinary Research.2011; 7(1): 54. CrossRef
Diagnosis and Molecular Characterization ofMycobacterium aviumsubsp.paratuberculosisfrom Dairy Cows in Colombia
J. A. Fernández-Silva, A. Abdulmawjood, M. Bülte
Veterinary Medicine International.2011; 2011: 1. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Evaluation of Three Molecular Methods of Repetitive Element Loci for Differentiation of Mycobacterium avium subsp. paratuberculosis (MAP)

J. Microbiol. 2009;47(3):253-259. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-008-0257-1

Related articles