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HOME > J. Microbiol > Volume 47(3); 2009 > Article
Research Support, Non-U.S. Gov't
Effect of Glycosylation on the Biochemical Properties of beta-Xylosidases from Aspergillus versicolor
Alexandre Favarin Somera 1, Marita Gimenez Pereira 1, Luis Henrique Souza Guimaraes 1, Maria de Lourdes Teixeira de Moraes Polizeli 1, Hector Francisco Terenzi 1, Rosa Prazeres Melo Furriel 2, Joao Atilio Jorge 1
Journal of Microbiology 2009;47(3):270-276.
DOI: https://doi.org/10.1007/s12275-008-0286-9
Published online: June 26, 2009
1Departamentos de Biologia e Quimica, Universidade de Sao Paulo, Avenida Bandeirantes, 3900, CEP 14040-901 Ribeirao Preto, SP, Brasil, 2Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Avenida Bandeirantes, 3900, CEP 14040-901 Ribeirau Preto, SP, Brasil
Corresponding author:  Joao Atilio Jorge , Tel: 55-16-3602-4679, 
Received: 21 November 2008   • Accepted: 4 March 2009
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Abstract

Aspergillus versicolor grown on xylan or xylose produces two beta-xylosidases with differences in biochemical properties and degree of glycosylation. We investigated the alterations in the biochemical properties of these beta-xylosidases after deglycosylation with Endo-H or PNGase F. After deglycosylation, both enzymes migrated faster in PAGE or SDS-PAGE exhibiting the same Rf. Temperature optimum of xylan-induced and xylose-induced beta-xylosidases was 45oC and 40oC, respectively, and 35oC after deglycosylation. The xylan- induced enzyme was more active at acidic pH. After deglycosylation, both enzymes had the same pH optimum of 6.0. Thermal resistance at 55oC showed half-life of 15 min and 9 min for xylose- and xylan-induced enzymes, respectively. After deglycosylation, both enzymes exhibited half-lives of 7.5 min. Native enzymes exhibited different responses to ions, while deglycosylated enzymes exhibited identical responses. Limited proteolysis yielded similar polypeptide profiles for the deglycosylated enzymes, suggesting a common polypeptide core with differential glycosylation apparently responsible for their biochemical and biophysical differences.

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    Effect of Glycosylation on the Biochemical Properties of beta-Xylosidases from Aspergillus versicolor
    J. Microbiol. 2009;47(3):270-276.   Published online June 26, 2009
    DOI: https://doi.org/10.1007/s12275-008-0286-9
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