Abstract
CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.
Citations
Citations to this article as recorded by
- Unraveling the biofilm armor: How ROS drives biofilm formation in aquatic pathogen Edwardsiella piscicida
Qingjian Fang, Yin Gou, Hanjie Gu, Qingjuan Wu, Jiaojiao He, Chenghua Li, Bin Sun, Yonghua Hu
Aquaculture.2025; 609: 742935. CrossRef - Microbial Contributions to Heavy Metal Phytoremediation in Agricultural Soils: A Review
Zobia Khatoon, Ma. del Carmen Orozco-Mosqueda, Gustavo Santoyo
Microorganisms.2024; 12(10): 1945. CrossRef - A Copper-Responsive Two-Component System Governs Lipoprotein Remodeling in Listeria monocytogenes
Gloria Komazin, Amena A. Rizk, Krista M. Armbruster, Victoria A. Bonnell, Manuel Llinás, Timothy C. Meredith, Mohamed Y. El-Naggar
Journal of Bacteriology.2023;[Epub] CrossRef - TCS regulator CpxR of Edwardsiella piscicida is vital for envelope integrity by regulating the new target gene yccA, stress resistance, and virulence
Qingjian Fang, Qingjuan Wu, Huiqin Huang, Jiarui Xu, Jianqiang Huang, Hanjie Gu, Yonghua Hu
Aquaculture.2023; 574: 739703. CrossRef - Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection
Dongmei Ma, Hanjie Gu, Yanjie Shi, Huiqin Huang, Dongmei Sun, Yonghua Hu
Frontiers in Microbiology.2021;[Epub] CrossRef - Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus
Majid Rasool Kamli, Nada A. Y. Alzahrani, Nahid H. Hajrah, Jamal S. M. Sabir, Adeel Malik
Microorganisms.2021; 9(3): 499. CrossRef - Edwardsiella piscicida HigB: A type II toxin that is essential to oxidative resistance, biofilm formation, serum survival, intracellular propagation, and host infection
Jinhong Xie, Qianyun Zhao, Huiqin Huang, Zaiguang Fang, Yonghua Hu
Aquaculture.2021; 535: 736382. CrossRef - Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site
Satya Prathyusha Bhamidimarri, Tessa R. Young, Muralidharan Shanmugam, Sandra Soderholm, Arnaud Baslé, Dirk Bumann, Bert van den Berg, Ann M. Stock
PLOS Biology.2021; 19(11): e3001446. CrossRef - The Two-Component System CopRS Maintains Subfemtomolar Levels of Free Copper in the Periplasm of Pseudomonas aeruginosa Using a Phosphatase-Based Mechanism
Lorena Novoa-Aponte, Cheng Xu, Fernando C. Soncini, José M. Argüello, Craig D. Ellermeier
mSphere.2020;[Epub] CrossRef - Characterization of Acinetobacter baumannii Copper Resistance Reveals a Role in Virulence
Caitlin L. Williams, Heather M. Neu, Yonas A. Alamneh, Ryan M. Reddinger, Anna C. Jacobs, Shweta Singh, Rania Abu-Taleb, Sarah L. J. Michel, Daniel V. Zurawski, D. Scott Merrell
Frontiers in Microbiology.2020;[Epub] CrossRef - The direct and indirect effects of environmental toxicants on the health of bumblebees and their microbiomes
Jason A. Rothman, Kaleigh A. Russell, Laura Leger, Quinn S. McFrederick, Peter Graystock
Proceedings of the Royal Society B: Biological Sciences.2020; 287(1937): 20200980. CrossRef - Changes in sediment microbial diversity following chronic copper-exposure induce community copper-tolerance without increasing sensitivity to arsenic
Ayanleh Mahamoud Ahmed, Vincent Tardy, Chloé Bonnineau, Patrick Billard, Stéphane Pesce, Emilie Lyautey
Journal of Hazardous Materials.2020; 391: 122197. CrossRef - Thioredoxin H (TrxH) contributes to adversity adaptation and pathogenicity of Edwardsiella piscicida
Bi-ying Wang, Hui-qin Huang, Shuang Li, Ping Tang, Hao-fu Dai, Jian-an Xian, Dong-mei Sun, Yong-hua Hu
Veterinary Research.2019;[Epub] CrossRef - HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida
Yan-Jie Shi, Qing-Jian Fang, Hui-Qin Huang, Chun-Guang Gong, Yong-Hua Hu
Veterinary Research.2019;[Epub] CrossRef - The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence
Saleh F. Alquethamy, Marjan Khorvash, Victoria G. Pederick, Jonathan J. Whittall, James C. Paton, Ian T. Paulsen, Karl A. Hassan, Christopher A. McDevitt, Bart A. Eijkelkamp
International Journal of Molecular Sciences.2019; 20(3): 575. CrossRef - Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants
Surajit Das, Hirak R. Dash, Jaya Chakraborty
Applied Microbiology and Biotechnology.2016; 100(7): 2967. CrossRef - Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity
Yuan-Yuan Sun, Heng Chi, Li Sun
Frontiers in Microbiology.2016;[Epub] CrossRef - Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
L Liu, H Chi, L Sun
Diseases of Aquatic Organisms.2015; 115(1): 67. CrossRef - Edwardsiella tarda Hfq: impact on host infection and global protein expression
Yong-hua Hu, Yong-xin Li, Li Sun
Veterinary Research.2014;[Epub] CrossRef - An Intimate Link: Two-Component Signal Transduction Systems and Metal Transport Systems in Bacteria
Kamna Singh, Dilani B Senadheera, Dennis G Cvitkovitch
Future Microbiology.2014; 9(11): 1283. CrossRef - Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
Sr Zhang, L Zhang, L Sun
Diseases of Aquatic Organisms.2014; 110(3): 181. CrossRef - The impact of transition metals on bacterial plant disease
Helen Fones, Gail M. Preston
FEMS Microbiology Reviews.2013; 37(4): 495. CrossRef - Overlapping and unique contributions of two conserved polysaccharide loci in governing distinct survival phenotypes in Vibrio vulnificus
Yunzhi Guo, Dean A. Rowe‐Magnus
Environmental Microbiology.2011; 13(11): 2888. CrossRef - Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain
Yun Sun, Chun-sheng Liu, Li Sun
Vaccine.2010; 28(40): 6603. CrossRef
MSK
Cite this Article
