Cloning, Expression, and Characterization of Thermostable Manganese Superoxide Dismutase from Thermoascus aurantiacus var. levisporus

Ning-Ning Song; Yan Zheng; Shi-Jin E; Duo-Chuan Li

doi:10.1007/s12275-008-0217-9

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Research Support, Non-U.S. Gov't
Cloning, Expression, and Characterization of Thermostable Manganese Superoxide Dismutase from Thermoascus aurantiacus var. levisporus: Ning-Ning Song ¹, Yan Zheng ^1,2, Shi-Jin E ¹, Duo-Chuan Li ¹; Journal of Microbiology 2009;47(1):123-130.
DOI: https://doi.org/10.1007/s12275-008-0217-9
Published online: February 20, 2009

¹Department of Environmental Biology, Shandong Agricultural University, Tai?n, Shandong 271018, P. R. China, ²Wanjie Mddical University, Zibo, Shandong, 255213, P. R. China¹Department of Environmental Biology, Shandong Agricultural University, Tai?n, Shandong 271018, P. R. China, ²Wanjie Mddical University, Zibo, Shandong, 255213, P. R. China

Corresponding author: Duo-Chuan Li , Tel: 86-538-824-9071,

Received: 4 September 2008 • Accepted: 1 December 2008

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Abstract

A superoxide dismutase (SOD) gene of Thermoascus aurantiacus var. levisporus, a thermophilic fungus, was cloned, sequenced, and expressed in Pichia pastoris and its gene product was characterized. The coding sequence predicted a 231 residues protein with a unique 35 amino acids extension at the N-terminus indicating a mitochondrial-targeting sequence. The content of Mn was 2.46 ug/mg of protein and Fe was not detected in the purified enzyme. The enzyme was found to be inhibited by NaN3, but not by KCN or H2O2. These results suggested that the SOD in Thermoascus aurantiacus var. levisporus was the manganese superoxide dismutase type. In comparison with other MnSODs, all manganese-binding sites were also conserved in the sequence (H88, H136, D222, H226). The molecular mass of a single band of the enzyme was estimated to be 21.7 kDa. The protein was expressed in tetramer form with molecular weight of 68.0 kDa. The activity of purified protein was 2,324 U/mg. The optimum temperature of the enzyme was 55oC and it exhibited maximal activity at pH 7.5. The enzyme was thermostable at 50 and 60oC and the half-life at 80oC was approximately 40 min.

Keywords: thermostable;MnSOD;cloning;expression;Thermoascus aurantiacus

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Cloning, Expression, and Characterization of Thermostable Manganese Superoxide Dismutase from Thermoascus aurantiacus var. levisporus

J. Microbiol. 2009;47(1):123-130. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0217-9

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