The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export

DongGeRaMi Moon; Jin-Ah Bae; Hyun Jin Cho; Jin Ho Yoon

doi:10.1007/s12275-008-0177-0

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The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export: DongGeRaMi Moon ¹, Jin-Ah Bae ², Hyun Jin Cho ¹, Jin Ho Yoon ¹; Journal of Microbiology 2008;46(4):422-428.
DOI: https://doi.org/10.1007/s12275-008-0177-0
Published online: August 31, 2008

¹Department of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea , ²Department of Education, Sungshin Women's University, Seoul 136-742, Republic of Korea¹Department of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea , ²Department of Education, Sungshin Women's University, Seoul 136-742, Republic of Korea

Corresponding author: Jin Ho Yoon , Tel: 82-2-920-7675,

Received: 14 July 2008 • Accepted: 22 July 2008

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Abstract

We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.

Keywords: mRNA export;Schizosaccharomyces pombe;spgle1

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Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef
Control of mRNA Export and Translation Termination by Inositol Hexakisphosphate Requires Specific Interaction with Gle1
Abel R. Alcázar-Román, Timothy A. Bolger, Susan R. Wente
Journal of Biological Chemistry.2010; 285(22): 16683. CrossRef
Generation of expression vectors for high-throughput functional analysis of target genes in Schizosaccharomyces pombe
Jiwon Ahn, Chung-Hae Choi, Chang-Mo Kang, Chun-Ho Kim, Hee-Moon Park, Kyung-Bin Song, Kwang-Lae Hoe, Misun Won, Kyung-Sook Chung
The Journal of Microbiology.2009; 47(6): 789. CrossRef

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The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export

J. Microbiol. 2008;46(4):422-428. Published online August 31, 2008

DOI: https://doi.org/10.1007/s12275-008-0177-0

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