Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens

Sung Hun Kim; Shukho Kim; Sung Guen Chun; Mi-Sun Park; Jeong Hyun Park; Bok-Kwon Lee

doi:10.1007/s12275-007-0197-1

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Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens: Sung Hun Kim ¹, Shukho Kim ¹, Sung Guen Chun ¹, Mi-Sun Park ¹, Jeong Hyun Park ², Bok-Kwon Lee ¹; Journal of Microbiology 2008;46(2):209-213.
DOI: https://doi.org/10.1007/s12275-007-0197-1
Published online: June 11, 2008

¹Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul 122-701, Republic of Korea, ²Department of Anatomy, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea

Corresponding author: Bok-Kwon Lee , Tel: 82-2-380-1462,

Received: 26 October 2007 • Accepted: 20 February 2008

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Abstract

We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.

Keywords: Salmonella Enteritidis;pulsed-field gel electrophoresis;phage typing;resistance

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Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens

J. Microbiol. 2008;46(2):209-213. Published online June 11, 2008

DOI: https://doi.org/10.1007/s12275-007-0197-1

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