Functional Analysis of pilQ Gene in Xanthomanas oryzae pv. oryzae, Bacterial Blight Pathogen of Rice

Seon-Hwa Lim; Byoung-Ho So; Ji-Chun Wang; Eun-Seong Song; Young-Jin Park; Byoung-Moo Lee; Hee-Wan Kang

doi:10.1007/s12275-007-0173-9

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Research Support, Non-U.S. Gov't
Functional Analysis of pilQ Gene in Xanthomanas oryzae pv. oryzae, Bacterial Blight Pathogen of Rice: Seon-Hwa Lim ¹, Byoung-Ho So ¹, Ji-Chun Wang ¹, Eun-Seong Song ¹, Young-Jin Park ², Byoung-Moo Lee ², Hee-Wan Kang ^1,3; Journal of Microbiology 2008;46(2):214-220.
DOI: https://doi.org/10.1007/s12275-007-0173-9
Published online: June 11, 2008

¹Graduate School of Biotechnology and Information Technology, Hankyong National University, Ansung 456-749, Republic of Korea, ²National Institute of Agricultural Biotechnology, Suwon 441-707, Republic of Korea, ³Institute of Genetic Engineering, Hankyoung National University, Ansung 456-749, Republic of Korea

Corresponding author: Hee-Wan Kang , Tel: 82-31-670-5420,

Received: 22 September 2007 • Accepted: 11 March 2008

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Abstract

Bacterial blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is the most devastating bacterial disease in rice. A virulence-attenuated mutant strain HNU89K9 of X. oryzae pv. oryzae (KACC10331), with a transposon insertion in the pilQ gene was used for this study. The pilQ was involved in the gene cluster pilMNOPQ of the Xoo genome. Growth rate of the pilQ mutant was similar to that of wild-type. At level of amino acids, PilQ of Xoo showed that a high sequence identities more than 94% and 70% to Xanthomonas species and to Xyllela fastidiosa, respectively but a low sequence homology less than 30% to other bacterial species. The twitching motility forming a marginal fringe on PSA media was observed on colony of the wild-type strain KACC10331, but not in mutant HNU89K9. Wild-type Xoo cells formed a biofilm on the surface of the PVC plastic test tube, while the mutant strain HNU89K9 did not form a biofilm. The results suggest that the pilQ gene of X. oryzae pv. oryzae plays a critical role in pathogenicity, twitching motility, and biofilm formation.

Keywords: Xanthomonas oryzae pv. oryzae;transposon;pilQ mutant;pathogenecity;twitching motility;biofilm

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Functional Analysis of pilQ Gene in Xanthomanas oryzae pv. oryzae, Bacterial Blight Pathogen of Rice

J. Microbiol. 2008;46(2):214-220. Published online June 11, 2008

DOI: https://doi.org/10.1007/s12275-007-0173-9

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