The Mutation of a Novel Saccharomyces cerevisiae SRL4 Gene Rescues the Lethality of rad53 and lcd1 Mutations by Modulating dNTP Levels

Do-Hee Choi; Young-Mi Oh; Sung-Hun Kwon; Sung-Ho Bae

doi:10.1007/s12275-008-0013-6

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The Mutation of a Novel Saccharomyces cerevisiae SRL4 Gene Rescues the Lethality of rad53 and lcd1 Mutations by Modulating dNTP Levels: Do-Hee Choi , Young-Mi Oh , Sung-Hun Kwon , Sung-Ho Bae; Journal of Microbiology 2008;46(1):75-80.
DOI: https://doi.org/10.1007/s12275-008-0013-6

Author Affiliations

Department of Biological Sciences, College of Natural Science, Inha University, Incheon 402-751, Republic of Korea

Corresponding author: Sung-Ho Bae , Tel: 82-32-860-7712,

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Abstract

The SRL4 (YPL033C) gene was initially identified by the screening of Saccharomyces cerevisiae genes that play a role in DNA metabolism and/or genome stability using the SOS system of Escherichia coli. In this study, we found that the srl4Δ; mutant cells were resistant to the chemicals that inhibit nucleotide metabolism and evidenced higher dNTP levels than were observed in the wild-type cells in the presence of hydroxyurea. The mutant cells also showed a significantly faster growth rate and higher dNTP levels at low temperature (16 oC) than were observed in the wild-type cells, whereas we detected no differences in the growth rate at 30oC. Furthermore, srl4Δ was shown to suppress the lethality of mutations of the essential S phase checkpoint genes, RAD53 and LCD1. These results indicate that SRL4 may be involved in the regulation of dNTP production by its function as a negative regulator of ribonucleotide reductase.

Keywords: SRL4;nucleotide metabolism;ribonucleotide reductase;SML1;checkpoint;Saccharomyces cerevisiae

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The Mutation of a Novel Saccharomyces cerevisiae SRL4 Gene Rescues the Lethality of rad53 and lcd1 Mutations by Modulating dNTP Levels

J. Microbiol. 2008;46(1):75-80.

DOI: https://doi.org/10.1007/s12275-008-0013-6

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