The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores

Yeo Jin Kim; Ji Yeon Kim; Ji Hye Kim; Seon Mee Yoon; Young-Bok Yoo; Se Won Yie

doi:10.1007/s12275-007-0171-y

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The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores: Yeo Jin Kim ¹, Ji Yeon Kim ¹, Ji Hye Kim ¹, Seon Mee Yoon ², Young-Bok Yoo ³, Se Won Yie ¹; Journal of Microbiology 2008;46(1):95-99.
DOI: https://doi.org/10.1007/s12275-007-0171-y

Author Affiliations

¹Department of Molecular Bioscience, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea, ²Gyengggido Agricultural Research and Extension Services/ Mushroom Research Institute, Rural Development Administration, Suwon 441-707, Republic of Korea, ³National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea

Corresponding author: Se Won Yie , Tel: 82-33-250-8544,

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Abstract

Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses.

Keywords: Pleurotus virus;dsRNA;fungal virus;virus distribution;cDNA

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The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores

J. Microbiol. 2008;46(1):95-99.

DOI: https://doi.org/10.1007/s12275-007-0171-y

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