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HOME > J. Microbiol > Volume 46(1); 2008 > Article
Research Support, Non-U.S. Gov't
The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores
Yeo Jin Kim 1, Ji Yeon Kim 1, Ji Hye Kim 1, Seon Mee Yoon 2, Young-Bok Yoo 3, Se Won Yie 1
Journal of Microbiology 2008;46(1):95-99.
DOI: https://doi.org/10.1007/s12275-007-0171-y
1Department of Molecular Bioscience, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Gyengggido Agricultural Research and Extension Services/ Mushroom Research Institute, Rural Development Administration, Suwon 441-707, Republic of Korea, 3National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea
Corresponding author:  Se Won Yie , Tel: 82-33-250-8544, 
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Abstract

Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses.

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    The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores
    J. Microbiol. 2008;46(1):95-99.
    DOI: https://doi.org/10.1007/s12275-007-0171-y
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