<i>FgVAC1</i> is an Essential Gene Required for Golgi-to-Vacuole Transport and Fungal Development in <i>Fusarium graminearum</i>

Sieun Kim; Jiyeun Park; You-Kyoung Han; Hokyoung Son

doi:10.1007/s12275-024-00160-x

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FgVAC1 is an Essential Gene Required for Golgi-to-Vacuole Transport and Fungal Development in Fusarium graminearum: Sieun Kim¹, Jiyeun Park², You-Kyoung Han¹, Hokyoung Son^3,4; Journal of Microbiology 2024;62(8):649-660.
DOI: https://doi.org/10.1007/s12275-024-00160-x
Published online: July 30, 2024

¹Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea
²Institute for Plant Sciences, University of Cologne, 50923, Cologne, Germany
³Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea
⁴Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea

Corresponding author: Hokyoung Son,
Email: hogongi7@snu.ac.kr.

Received: 5 July 2024 • Revised: 11 July 2024 • Accepted: 14 July 2024

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Abstract

Fusarium graminearum is an important plant pathogen that causes head blight in cereal crops such as wheat, barley, and rice worldwide. In this study, we identified and functionally characterized FgVAC1, an essential gene in F. graminearum that encodes a Rab5 effector involved in membrane tethering functions. The essentiality of FgVAC1 was confirmed through a conditional promoter replacement strategy using the zearalenone-inducible promoter (P_ZEAR). Cytological analyses revealed that FgVac1 colocalizes with FgRab51 on early endosomes and regulates the proper transport of the vacuolar hydrolase FgCpy1 to the vacuole. Suppression of FgVAC1 led to inhibited vegetative growth, reduced asexual and sexual reproduction, decreased deoxynivalenol (DON) biosynthesis, and diminished pathogenicity. Our findings highlight the significant role of FgVac1 in vacuolar protein sorting, fungal development, and plant infection in F. graminearum.

Keywords: Fusarium graminearum · FgVac1 · Vacuolar protein sorting · Endosome

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FgVAC1 is an Essential Gene Required for Golgi-to-Vacuole Transport and Fungal Development in Fusarium graminearum

J. Microbiol. 2024;62(8):649-660. Published online July 30, 2024

DOI: https://doi.org/10.1007/s12275-024-00160-x

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