Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes

Chang Yan; Jeffrey S. Owen; Eun-Young Seo; Dawoon Jung; Shan He

doi:10.1007/s12275-023-00063-3

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Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes: Chang Yan ^1,2, Jeffrey S. Owen ³, Eun-Young Seo ², Dawoon Jung ², Shan He ^2,4; Journal of Microbiology 2023;61(7):655-662.
DOI: https://doi.org/10.1007/s12275-023-00063-3
Published online: August 17, 2023

¹Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, People’s Republic of China, ²Ningbo Institute of Marine Medicine, Peking University, Ningbo 315832, People’s Republic of China, ³Department of Environmental Science, Hankuk University of Foreign Studies, Yongin 17035, Republic of Korea, ⁴Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, People’s Republic of Chin

Corresponding author: Dawoon Jung ,
Shan He ,

Received: 12 May 2023 • Revised: 13 June 2023 • Accepted: 18 June 2023

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Abstract

Pure cultivation of microbes is still limited by the challenges of microbial uncultivability, with most microbial strains unable to be cultivated under standard laboratory conditions. The experience accumulated from advanced techniques such as in situ cultivation has identified that microbial interactions exist in natural habitats but are absent in laboratory cultures. These microbial interactions are likely one of the key factors in isolating previously uncultured microbes. The need for better knowledge of the mechanisms operating in microbial interactions has led to various experiments that have utilized microbial interactions in different approaches to microbial cultivation. These new attempts to understand microbial interactions not only present a new perspective on microbial uncultivability but also provide an opportunity to access uncultured phylogenetically novel microbes with their potential biotechnology applications. In this review, we focus on studies of the mechanisms of microbial interaction where the growth of other microbes is affected. Additionally, we review some successful applications of microbial interactions in cultivation methods, an approach that can play an important role in the bioprospecting of untapped microbial resources.

Keywords: Uncultured microbes · Microbial uncultivability · Microbial cultivation · Growth factors

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Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes

J. Microbiol. 2023;61(7):655-662. Published online August 17, 2023

DOI: https://doi.org/10.1007/s12275-023-00063-3

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