Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola

Qi Liu; Jun Yang; Waqar Ahmed; Xiaoyan Wan; Lanfang Wei; Guanghai Ji

doi:10.1007/s12275-022-1542-0

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Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola: Qi Liu ^1,2, Jun Yang ^1,2,3, Waqar Ahmed ^1,2, Xiaoyan Wan ^1,2, Lanfang Wei ^1,2, Guanghai Ji ^1,2; Journal of Microbiology 2022;60(5):496-510.
DOI: https://doi.org/10.1007/s12275-022-1542-0
Published online: March 31, 2022

¹State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, P. R. China, ²Key Laboratory of Agro-Biodiversity and Pest Management of Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China, ³College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, P. R. China

Corresponding author: Guanghai Ji ,

Received: 19 October 2021 • Revised: 24 January 2022 • Accepted: 28 January 2022

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Abstract

Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most destructive diseases affecting rice production worldwide. In this study, we extracted and purified phenazine substances from the secondary metabolites of Lysobacter antibioticus 13-6. The bacteriostatic mechanism of phenazine substances against Xoc was investigated through physiological response and transcriptomic analysis.
Results
showed that phenazine substances affects the cell membrane permeability of Xoc, which causes cell swelling and deformation, blockage of flagellum synthesis, and imbalance of intracellular environment. The changes in intracellular environment affect the physiological and metabolic functions of Xoc, which reduces the formation of pathogenic factors and pathogenicity. Through transcriptomic analysis, we found that among differentially expressed genes, the expression of 595 genes was induced significantly (275 up-regulated and 320 down-regulated). In addition, we observed that phenazine substances affects three main functions of Xoc, i.e., transmembrane transporter activity, DNA-mediated transposition, and structural molecular activity. Phenazine substances also inhibits the potassium ion transport system that reduces Xoc resistance and induces the phosphate ion transport system to maintain the stability of the internal environment. Finally, we conclude that phenazine substances could retard cell growth and reduce the pathogenicity of Xoc by affecting cell structure and physiological metabolism. Altogether, our study highlights latest insights into the antibacterial mechanism of phenazine substances against Xoc and provides basic guidance to manage the incidence of bacterial leaf streak of rice.

Keywords: Lysobacter antibioticus, Xanthomonas oryzae pv.oryzicola, biological control, phenazine substances, transcriptomic analysis

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Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola

J. Microbiol. 2022;60(5):496-510. Published online March 31, 2022

DOI: https://doi.org/10.1007/s12275-022-1542-0

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