A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger

Da Tian; Liyan Wang; Jun Hu; Liangliang Zhang; Ningning Zhou; Jingjing Xia; Meiyue Xu; Kianpoor Kalkhajeh Yusef; Shimei Wang; Zhen Li; Hongjian Gao

doi:10.1007/s12275-021-1178-5

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A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger: Da Tian ^1,2†, Liyan Wang ^1,2†, Jun Hu ^1,2, Liangliang Zhang ^1,2, Ningning Zhou ^1,2, Jingjing Xia ^1,2, Meiyue Xu ^1,2, Kianpoor Kalkhajeh Yusef ^1,2, Shimei Wang ^3,4, Zhen Li ^3,4, Hongjian Gao ^1,2; Journal of Microbiology 2021;59(9):819-826.
DOI: https://doi.org/10.1007/s12275-021-1178-5
Published online: August 12, 2021

¹Anhui Province Key Laboratory of Farmland Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, P. R. China, ²Research Centre of Phosphorus Efficient Utilization and Water Environment Protection along the Yangtze River Economic Belt, Anhui Agricultural University, Hefei 230036, P. R. China, ³College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P. R. China, ⁴Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, P. R. China

Corresponding author: Da Tian , Tel: +86-0551-65786447,
Hongjian Gao , Tel: +86-0551-65786447,

Received: 5 April 2021 • Revised: 1 July 2021 • Accepted: 2 July 2021

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Abstract

Phosphate solubilizing fungi (PSF) have been widely applied to dissolve insoluble phosphates (IPs). However, the PSF usually demonstrates a different phosphate solubilizing capacity for various IPs. This study explored the mechanisms of Aspergillus niger for the dissolution of ferric phosphate (FePO4, Fe-P), and tricalcium phosphate (Ca3[PO4]2, Ca-P) regarding the tricarboxylic acid (TCA) cycle. Aspergillus niger has higher phosphorus (P) content released from Ca-P, reached the maximum value of 861 mg/L after seven days of incubation, compared with the 169 mg/L from Fe-P. Oxalic acid promoted the release of P from Ca-P through the formation of calcium oxalate. The presence of Fe-P can stimulate A. niger to secrete large amounts of citric acid, confirmed by the enhancement of citrate synthase (CS) activity. However, citric acid only promotes 0.5% of P released from Fe-P. Meanwhile, although oxalic acid still dominates the release of P from Fe-P, its abundance was significantly declined. In contrast, oxalic acid also shows a higher P release ratio in Ca-P than citric acid, i.e., 36% vs. 22%. This study points to the future usage of A. niger to dissolve IPs in soil required to enhance oxalic acid secretion.

Keywords: Aspergillus niger, insoluble phosphates, organic acids, phosphorus release, TCA cycle

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A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger

J. Microbiol. 2021;59(9):819-826. Published online August 12, 2021

DOI: https://doi.org/10.1007/s12275-021-1178-5

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