NF-κB/ROS and ERK pathways regulate NLRP3 inflammasome activation in Listeria monocytogenes infected BV2 microglia cells

Lin Yuan; Yurong Zhu; Shuang Huang; Lin Lin; Xugan Jiang; Shengxia Chen

doi:10.1007/s12275-021-0692-9

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NF-κB/ROS and ERK pathways regulate NLRP3 inflammasome activation in Listeria monocytogenes infected BV2 microglia cells: Lin Yuan ^1,2, Yurong Zhu ^1,3, Shuang Huang ¹, Lin Lin ¹, Xugan Jiang ¹, Shengxia Chen ¹; Journal of Microbiology 2021;59(8):771-781.
DOI: https://doi.org/10.1007/s12275-021-0692-9
Published online: June 1, 2021

¹School of Medicine, Jiangsu University, Zhenjiang 212013, P. R. China, ²Department of Clinical Laboratory, Northern Jiangsu People' s Hospital, Yangzhou 225001, P. R. China, ³Department of Microbiology Laboratory, Linfen Central Hospital, Linfen 041000, P. R. China¹School of Medicine, Jiangsu University, Zhenjiang 212013, P. R. China, ²Department of Clinical Laboratory, Northern Jiangsu People' s Hospital, Yangzhou 225001, P. R. China, ³Department of Microbiology Laboratory, Linfen Central Hospital, Linfen 041000, P. R. China

Corresponding author: Shengxia Chen ,

Received: 28 December 2020 • Revised: 12 April 2021 • Accepted: 10 May 2021

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Abstract

Listeria monocytogenes is a food-borne pathogen responsible for neurolisteriosis, which is potentially lethal in immunocompromised individuals. Microglia are the main target cells for L. monocytogenes in central nervous system (CNS). However, the precise mechanisms by which they trigger neuroinflammatory processes remain unknown. The BV2 microglial cell line and a murine model of L. monocytogenes infection were used for experiments in this study. Listeria monocytogenes induced pyroptosis and nucleotide binding and oligomerization, leucine-rich repeat, pyrin domain-containing 3 (NLRP3) inflammasome activation in BV2. Pharmacological inhibition of the NLRP3 inflammasome attenuated L. monocytogenes- induced pyroptosis. Moreover, inhibition of nuclear factor kappa-B (NF-κB) and extracellular regulated protein kinases (ERK) pathways induced a decrease in caspase1 activation and mature IL-1β-17 secretion. Our collective findings support critical involvement of the NLRP3 inflammasome in L. monocytogenes-induced neuroinflammation and, to an extent, ROS production. In addition, ERK and NF-κB signaling play an important role in activation of the NLRP3 inflammasome, both in vitro and in vivo.

Keywords: KIF20A;influenza A virus;paprotrain

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NF-κB/ROS and ERK pathways regulate NLRP3 inflammasome activation in Listeria monocytogenes infected BV2 microglia cells

J. Microbiol. 2021;59(8):771-781. Published online June 1, 2021

DOI: https://doi.org/10.1007/s12275-021-0692-9

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