The osmotic stress response operon betIBA is under the functional regulation of BetI and the quorum-sensing regulator AnoR in Acinetobacter nosocomialis

Bindu Subhadra; Surya Surendran; Bo Ra Lim; Jong Sung Yim; Dong Ho Kim; Kyungho Woo; Hwa-Jung Kim; Man Hwan Oh; Chul Hee Choi

doi:10.1007/s12275-020-0186-1

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The osmotic stress response operon betIBA is under the functional regulation of BetI and the quorum-sensing regulator AnoR in Acinetobacter nosocomialis: Bindu Subhadra ^1,2, Surya Surendran ^1,2, Bo Ra Lim ^1,2, Jong Sung Yim ^1,2, Dong Ho Kim ^1,2, Kyungho Woo ^1,2, Hwa-Jung Kim ^1,2, Man Hwan Oh ³, Chul Hee Choi ^1,2; Journal of Microbiology 2020;58(6):519-529.
DOI: https://doi.org/10.1007/s12275-020-0186-1
Published online: May 27, 2020

¹Department of Microbiology, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea, ²Department of Medical Science, Chungnam National University, Daejeon 34134, Republic of Korea, ³Department of Nanobiomedical Science, Dankook University, Cheonan 31116, Republic of Korea

Corresponding author: Man Hwan Oh , Tel: +82-42-580-8246,
Chul Hee Choi , Tel: +82-42-580-8246,

Received: 7 April 2020 • Revised: 28 April 2020 • Accepted: 12 May 2020

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Abstract

Adaptation to changing environmental conditions is crucial for the survival of microorganisms. Bacteria have evolved various mechanisms to cope with osmotic stress. Here, we report the identification and functional characterization of the osmotic stress response operon, betIBA, in Acinetobacter nosocomialis. The betIBA operon encodes enzymes that are important for the conversion of choline to the osmoprotectant, glycine betaine. The betIBA operon is polycistronic and is under the regulation of the first gene, betI, of the same operon. A bioinformatics analysis revealed the presence of a BetI-binding motif upstream of the betIBA operon, and electrophoretic mobility shift assays confirmed the specific binding of BetI. An mRNA expression analysis revealed that expression of betI, betB, and betA genes is elevated in a betIeletion mutant compared with the wild type, confirming that the autorepressor BetI represses the betIBA operon in A. nosocomialis. We further found that the betIBA operon is under the transcriptional control of the quorum-sensing (QS) regulator, AnoR in, A. nosocomialis. A subsequent analysis of the impact of BetI on expression of the QS genes, anoR and anoI, demonstrated that BetI acts as a repressor of anoR and anoI. In addition, it was noticed that the osmotic stress response regulator, OmpR might play an important role in controlling the expression of betIBA operon in A. nosocomialis. Collectively, these data demonstrate that QS and osmotic stress-response systems are correlated in A. nosocomialis and that the expression of genes in both systems is finely tuned by various feedback loops depending on osmolarity conditions.

Keywords: Acinetobacter nosocomialis, osmotic stress, quorum sensing, BetI

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The osmotic stress response operon betIBA is under the functional regulation of BetI and the quorum-sensing regulator AnoR in Acinetobacter nosocomialis

J. Microbiol. 2020;58(6):519-529. Published online May 27, 2020

DOI: https://doi.org/10.1007/s12275-020-0186-1

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