Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge

Jae Kyu Lim; Yun Jae Kim; Jhung-Ahn Yang; Teddy Namirimu; Sung-Hyun Yang; Mi-Jeong Park; Yong Min Kwon; Hyun Sook Lee; Sung Gyun Kang; Jung-Hyun Lee; Kae Kyoung Kwon

doi:10.1007/s12275-020-9424-9

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Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge: Jae Kyu Lim ^1,2, Yun Jae Kim ^1,2, Jhung-Ahn Yang ¹, Teddy Namirimu ¹, Sung-Hyun Yang ¹, Mi-Jeong Park ^1,2, Yong Min Kwon ³, Hyun Sook Lee ^1,2, Sung Gyun Kang ^1,2, Jung-Hyun Lee ^1,2, Kae Kyoung Kwon ^1,2; Journal of Microbiology 2020;58(4):260-267.
DOI: https://doi.org/10.1007/s12275-020-9424-9
Published online: April 1, 2020

¹Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea, ²KIOST School, University of Science and Technology, Daejeon 34113, Republic of Korea, ³Department of Applied Research, National Marine Biodiversity Institute of Korea, Chungcheongnam-do 33662, Republic of Korea

Corresponding author: Kae Kyoung Kwon , Tel: +82-51-664-3371,

Received: 3 September 2019 • Revised: 30 September 2019 • Accepted: 23 January 2020

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Abstract

A strictly anaerobic, dissimilatory Fe(III)-reducing hyperthermophilic archaeon, designated as strain IOH1T, was isolated from a new deep-sea hydrothermal vent (Onnuri Vent Field) area in the Central Indian Ocean ridge. Strain IOH1T showed > 99% 16S rRNA gene sequence similarity with Thermococcus celericrescens TS2T (99.4%) and T. siculi DSM 12349T (99.2%). Additional three species T. barossii SHCK-94T (99.0%), T. celer Vu13T (98.8%), and T. piezophilus (98.6%) showed > 98.6% of 16S rRNA gene sequence similarity, however, the maximum OrthoANI value is 89.8% for the genome of T. celericrescens TS2T. Strain IOH1T cells are coccoid, 1.2–1.8 μm in diameter, and motile by flagella. Growth was at 70–82°C (optimum 80°C), pH 5.4–8.0 (optimum pH 6.0) with 2–4% (optimum 3%) NaCl. Growth of strain IOH1T was enhanced by starch, pyruvate, D(+)-maltose and maltodextrin as a carbon sources, and elemental sulfur as an electron acceptor; clearly different from those of related species T. celecrescens DSM 17994T and T. siculi DSM 12349T. Strain IOH1T, T. celercrescence DSM 17994T, and T. siculi DSM 12349T reduced soluble Fe(III)-citrate present in the medium, whereas the amount of total cellular proteins increased with the concomitant accumulation of Fe(II). We determined a circular chromosome of 2,234 kb with an extra-chromosomal archaeal plasmid, pTI1, of 7.7 kb and predicted 2,425 genes. The DNA G + C content was 54.9 mol%. Based on physiological properties, phylogenetic, and genome analysis, we proposed that strain IOH1T (= KCTC 15844T = JCM 39077T) is assigned to a new species in the genus Thermococcus and named Thermococcus indicus sp. nov.

Keywords: Thermococcus indicus sp. nov., Fe(III)-reduction, hyperthermophile, Indian Ocean, Onnuri Vent Field

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Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge

J. Microbiol. 2020;58(4):260-267. Published online April 1, 2020

DOI: https://doi.org/10.1007/s12275-020-9424-9

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