Kinetic characterization of laccase from Bacillus atrophaeus, and its potential in juice clarification in free and immobilized forms

Lokesh Kumar Narnoliya; Neera Agarwal; Satya N. Patel; Sudhir P. Singh

doi:10.1007/s12275-019-9170-z

Articles

Page Path: HOME > J. Microbiol > Volume 57(10); 2019 > Article

Journal Article
Kinetic characterization of laccase from Bacillus atrophaeus, and its potential in juice clarification in free and immobilized forms: Lokesh Kumar Narnoliya ¹, Neera Agarwal ^1,2, Satya N. Patel ^1,2, Sudhir P. Singh ¹; Journal of Microbiology 2019;57(10):900-909.
DOI: https://doi.org/10.1007/s12275-019-9170-z
Published online: August 28, 2019

¹Center of Innovative and Applied Bioprocessing, S.A.S. Nagar, Mohali-140 306, India, ²Panjab University, Chandigarh, India¹Center of Innovative and Applied Bioprocessing, S.A.S. Nagar, Mohali-140 306, India, ²Panjab University, Chandigarh, India

Corresponding author: Sudhir P. Singh , Tel: +91-172-522-1415,

Received: 3 April 2019 • Revised: 23 May 2019 • Accepted: 24 May 2019

prev next

51 Views
0 Download
17 Web of Science
15 Crossref
21 Scopus

Full Article

Abstract

In the present study, a laccase gene (BaLc) from a lignin degrading bacterium, Bacillus atrophaeus, has been cloned and expressed in Escherichia coli. The optimal catalytic activity of the protein was achieved at 5.5 pH and 35°C temperature, measured by oxidation of ABTS. The Km and Vmax values were determined as 1.42 mM and 4.16 μmole/min, respectively. To achieve the enzyme recovery, the biocatalyst (BaLc) was covalently attached onto the functionalized iron magnetic-nanoparticles. The nanoparticles were characterized by zeta-potential and FTIR analyses. The immobilized BaLc enzyme was physico-kinetically characterized, exhibiting retention of 60% of the residual activity after ten reaction cycles of ABTS oxidation. The immobilized biocatalyst system was tested for its biotechnological exploitability in plant juice processing, achieving 41–58% of phenol reduction, 41–58% decolorization, 50–59% turbidity reduction in the extracts of banana pseudo-stem and sweet sorghum stalk, and apple fruit juice. This is the first study to demonstrate the use of nanoparticle- laccase conjugate in juice clarification. The findings suggest that B. atrophaus laccase is a potential catalytic tool for plant juice bioprocessing activities.

Keywords: laccase;Bacillus atrophaeus;immobilization;magnetic nanoparticles;fruit juice clarification

Figure & Data

References

Citations

Citations to this article as recorded by

Immobilized enzymes: exploring its potential in food industry applications
K. A. Jothyswarupha, Swethaa Venkataraman, Devi Sri Rajendran, S. S. Sakthi Shri, Shivani Sivaprakasam, Tholeti Yamini, P. Karthik, Vaidyanathan Vinoth Kumar
Food Science and Biotechnology.2024;[Epub] CrossRef
Harnessing the power of bacterial laccases for xenobiotic degradation in water: A 10-year overview
Mujeeb ur Rahman, Muhammad Wajid Ullah, Junaid Ali Shah, Sivasamy Sethupathy, Hazart Bilal, Sidikov Akmal Abdikakharovich, Afaq Ullah Khan, Khalid Ali Khan, Noureddine Elboughdiri, Daochen Zhu
Science of The Total Environment.2024; 918: 170498. CrossRef
Laccase-mediated degradation of emerging contaminants: unveiling a sustainable solution
Pooja Thathola, Elda M. Melchor-Martínez, Priyanka Adhikari, Saúl Antonio Hernández Martínez, Anita Pandey, Roberto Parra-Saldívar
Environmental Science: Advances.2024; 3(11): 1500. CrossRef
Biochemical Characteristics of Laccases and Their Practical Application in the Removal of Xenobiotics from Water
Agnieszka Gałązka, Urszula Jankiewicz, Andrzej Szczepkowski
Applied Sciences.2023; 13(7): 4394. CrossRef
Application of Immobilized Enzymes in Juice Clarification
Feng Wang, Hui Xu, Miaomiao Wang, Xiaolei Yu, Yi Cui, Ling Xu, Anzhou Ma, Zhongyang Ding, Shuhao Huo, Bin Zou, Jingya Qian
Foods.2023; 12(23): 4258. CrossRef
Bacterial Laccases as Biocatalysts for the Remediation of Environmental Toxic Pollutants: A Green and Eco-Friendly Approach—A Review
Neha Agarwal, Vijendra Singh Solanki, Amel Gacem, Mohd Abul Hasan, Brijesh Pare, Amrita Srivastava, Anupama Singh, Virendra Kumar Yadav, Krishna Kumar Yadav, Chaigoo Lee, Wonjae Lee, Sumate Chaiprapat, Byong-Hun Jeon
Water.2022; 14(24): 4068. CrossRef
Enzymatic treatment, unfermented and fermented fruit-based products: current state of knowledge
Joanne Yi Hui Toy, Yuyun Lu, Dejian Huang, Keisuke Matsumura, Shao-Quan Liu
Critical Reviews in Food Science and Nutrition.2022; 62(7): 1890. CrossRef
Current research progress on laccase-like nanomaterials
Lulu Lei, Xiaoyu Yang, Yudong Song, Hui Huang, Yongxin Li
New Journal of Chemistry.2022; 46(8): 3541. CrossRef
Poly(vinyl Alcohol)-Alginate Immobilized Trametes versicolor IBL-04 Laccase as Eco-friendly Biocatalyst for Dyes Degradation
Sadia Noreen, Muhammad Asgher, Sarmad Ahmad Qamar, Muhammad Bilal, Hafiz M. N. Iqbal
Catalysis Letters.2022; 152(6): 1869. CrossRef
Recent developments in enzyme immobilization technology for high-throughput processing in food industries
Asghar Taheri-Kafrani, Sara Kharazmi, Mahmoud Nasrollahzadeh, Asieh Soozanipour, Fatemeh Ejeian, Parisa Etedali, Hajar-Alsadat Mansouri-Tehrani, Amir Razmjou, Samaneh Mahmoudi-Gom Yek, Rajender S. Varma
Critical Reviews in Food Science and Nutrition.2021; 61(19): 3160. CrossRef
Laccases in food processing: Current status, bottlenecks and perspectives
Emanueli Backes, Camila Gabriel Kato, Rúbia Carvalho Gomes Corrêa, Regina de Fátima Peralta Muniz Moreira, Rosely Aparecida Peralta, Lillian Barros, Isabel C.F.R. Ferreira, Gisella Maria Zanin, Adelar Bracht, Rosane Marina Peralta
Trends in Food Science & Technology.2021; 115: 445. CrossRef
A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement
Peter Adewale, Alice Lang, Fang Huang, Daochen Zhu, Jianzhong Sun, Michael Ngadi, Trent Chunzhong Yang
Scientific Reports.2021;[Epub] CrossRef
Laccases as green and versatile biocatalysts: from lab to enzyme market—an overview
Tatiane Brugnari, Dayane Moreira Braga, Camila Souza Almeida dos Santos, Bruno Henrique Czelusniak Torres, Tatiani Andressa Modkovski, Charles Windson Isidoro Haminiuk, Giselle Maria Maciel
Bioresources and Bioprocessing.2021;[Epub] CrossRef
Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology
Sihai Han, Chonlong Chio, Tianxiao Ma, Aristide Laurel Mokale Kognou, Sarita Shrestha, Feifei Chen, Wensheng Qin
Biofuels, Bioproducts and Biorefining.2021; 15(3): 867. CrossRef
Magnetic nanobiocatalyst for extraction of bioactive ingredients: A novel approach
Shamraja S. Nadar, Pravin D. Patil, Nanda M. Rohra
Trends in Food Science & Technology.2020; 103: 225. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Kinetic characterization of laccase from Bacillus atrophaeus, and its potential in juice clarification in free and immobilized forms

J. Microbiol. 2019;57(10):900-909. Published online August 28, 2019

DOI: https://doi.org/10.1007/s12275-019-9170-z

Related articles