Mutants defective in the production of encapsulin show a tan-phaselocked phenotype in Myxococcus xanthus

Dohee Kim; Juo Choi; Sunjin Lee; Hyesook Hyun; Kyoung Lee; Kyungyun Cho

doi:10.1007/s12275-019-8683-9

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Mutants defective in the production of encapsulin show a tan-phaselocked phenotype in Myxococcus xanthus: Dohee Kim ¹, Juo Choi ¹, Sunjin Lee ¹, Hyesook Hyun ¹, Kyoung Lee ², Kyungyun Cho ¹; Journal of Microbiology 2019;57(9):795-802.
DOI: https://doi.org/10.1007/s12275-019-8683-9
Published online: June 11, 2019

¹Department of Biotechnology, Hoseo University, Asan 31499, Republic of Korea, ²Department of Microbiology, Changwon National University, Changwon 51140, Republic of Korea

Corresponding author: Kyungyun Cho , Tel: +82-41-540-5627,

Received: 11 December 2018 • Revised: 6 March 2019 • Accepted: 8 April 2019

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Abstract

Myxococcus xanthus, a myxobacterium, displays phase variation between yellow phase and tan phase. We found that deletion of the encA gene encoding encapsulin and the encF gene encoding a metalloprotease causes formation of tan colonies that never transform into yellow colonies. The encA and encF mutants were defective in the production of DKxanthene and myxovirescin. They did not produce extracellular polysaccharides; hence, the cells did not aggregate in liquid and showed reduced swarming on agar plates. The mutants had defective sporulation, but were rescued extracellularly by wild type cells. All these traits indicate that the encA and encF mutants are likely to be tan-phase-locked, and encapsulin has a close relationship with phase variation in M. xanthus. The encA and encF genes are localized in the same gene cluster, encBAEFG (MXAN_3557~MXAN_3553). Unlike the encA and encF genes, deletion of other genes in the cluster did not show tan-phase-locked phenotype.

Keywords: myxobacteria;Myxococcus xanthus;encapsulin;phase variation

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Mutants defective in the production of encapsulin show a tan-phaselocked phenotype in Myxococcus xanthus

J. Microbiol. 2019;57(9):795-802. Published online June 11, 2019

DOI: https://doi.org/10.1007/s12275-019-8683-9

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