Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1

Mostafa N. Taha; Amal E. Saafan; A. Ahmedy; Eman El Gebaly; Ahmed S. Khairalla

doi:10.1007/s12275-019-8548-2

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Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1: Mostafa N. Taha ¹, Amal E. Saafan ², A. Ahmedy ³, Eman El Gebaly ⁴, Ahmed S. Khairalla ⁴; Journal of Microbiology 2019;57(7):618-625.
DOI: https://doi.org/10.1007/s12275-019-8548-2
Published online: June 27, 2019

¹Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt, ²Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Menoufia, Egypt, ³Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, ⁴Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt¹Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt, ²Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Menoufia, Egypt, ³Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, ⁴Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

Corresponding author: Mostafa N. Taha , Tel: +20-01019576104,

Received: 1 October 2018 • Revised: 10 January 2019 • Accepted: 30 January 2019

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Abstract

Quorum sensing (QS) regulates virulence factor expression in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence factors without inhibiting the growth of P. aeruginosa is a promising approach for overcoming the widespread resistance of P. aeruginosa. This study was proposed to investigate the effects of two novel synthetic peptides on the biofilm development and virulence factor production of P. aeruginosa. The tested strain was P. aeruginosa PAO1. The results indicated that both of the synthetic peptides (LIVRHK and LIVRRK) inhibited (P < 0.05) the formation of biofilms and the production of virulence factors, including pyocyanin, protease, and rhamnolipids, without inhibiting the growth of PAO1. Additionally, we detected transcriptional changes related to QS and found a significant reduction in the levels of gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates that LIVRRK and LIVRHK are novel synthetic peptides that can act as potent inhibitors of QS-regulated virulence factors in P. aeruginosa. Moreover, these synthetic peptides have potential applications in the treatment of biofilmrelated diseases. Both peptides may be able to control chronic infections and biofilm-associated problems of P. aeruginosa.

Keywords: biofilm;peptides;quorum sensing;virulence factors

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Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1

J. Microbiol. 2019;57(7):618-625. Published online June 27, 2019

DOI: https://doi.org/10.1007/s12275-019-8548-2

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