A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

Jin-Il Park; Sun-Min Seo; Jong-Hyung Park; Hee-Yeon Jeon; Jun-Young Kim; Seung-Hyun Ryu; Yang-Kyu Choi

doi:10.1007/s12275-018-7504-x

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A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium: Jin-Il Park ^1,2, Sun-Min Seo ¹, Jong-Hyung Park ¹, Hee-Yeon Jeon ^1,3, Jun-Young Kim ¹, Seung-Hyun Ryu ^1,4, Yang-Kyu Choi ¹; Journal of Microbiology 2018;56(4):272-279.
DOI: https://doi.org/10.1007/s12275-018-7504-x
Published online: April 2, 2018

¹Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea, ²ViroMed Co. Ltd., Seoul 08826, Republic of Korea, ³Department of Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea, ⁴Samjin Pharmaceutical Co., Ltd., Seoul 04054, Republic of Korea¹Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea, ²ViroMed Co. Ltd., Seoul 08826, Republic of Korea, ³Department of Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea, ⁴Samjin Pharmaceutical Co., Ltd., Seoul 04054, Republic of Korea

Corresponding author: Yang-Kyu Choi , Tel: +82-2-2049-6113,

Received: 16 November 2017 • Revised: 29 January 2018 • Accepted: 4 February 2018

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Abstract

Adult mice were treated with dextran sulfate sodium (DSS) and infected with Citrobacter rodentium for developing a novel murine colitis model. C57BL/6N mice (7-week-old) were divided into four groups. Each group composed of control, dextran sodium sulfate-treated (DSS), C. rodentiuminfected (CT), and DSS-treated and C. rodentium-infected (DSS-CT) mice. The DSS group was administered 1% DSS in drinking water for 7 days. The CT group was supplied with normal drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The DSS-CT group was supplied with 1% DSS in drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The mice were sacrificed 10 days after the induction of C. rodentium infection. The DSS-CT group displayed significantly shorter colon length, higher spleen to body weight ratio, and higher histopathological score compared to the other three groups. The mRNA expression levels of tumor necrosis factor (TNF)-α and interferon (INF)-γ were significantly upregulated; however, those of interleukin (IL)-6 and IL-10 were significantly downregulated in the DSS-CT group than in the control group. These results demonstrated that a combination of low DSS concentration (1%) and C. rodentium infection could effectively induce inflammatory bowel disease (IBD) in mice. This may potentially be used as a novel IBD model, in which colitis is induced in mice by the combination of a chemical and a pathogen.

Keywords: Citrobacter rodentium;colitis;dextran sulfate sodium

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A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

J. Microbiol. 2018;56(4):272-279. Published online April 2, 2018

DOI: https://doi.org/10.1007/s12275-018-7504-x

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