Morphologies and phenotypes in Bacillus subtilis biofilms

Xiaoling Wang; Shuo Meng; Jingshi Han

doi:10.1007/s12275-017-7041-z

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Morphologies and phenotypes in Bacillus subtilis biofilms: Xiaoling Wang ^1,2, Shuo Meng ¹, Jingshi Han ¹; Journal of Microbiology 2017;55(8):619-627.
DOI: https://doi.org/10.1007/s12275-017-7041-z
Published online: July 4, 2017

¹School of Mechanical Engineering, University of Science and Technology, Beijing, P. R. China, ²John A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138, USA¹School of Mechanical Engineering, University of Science and Technology, Beijing, P. R. China, ²John A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138, USA

Corresponding author: Xiaoling Wang , Tel: .,

Received: 26 January 2017 • Revised: 29 May 2017 • Accepted: 2 June 2017

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Abstract

In this study, we explored Bacillus subtilis biofilm growth under various conditions such as the use of substrates with different stiffnesses and nutrient levels using a well-developed optical imaging technique to spatially and temporally track biofilm growth. We also developed a quantitative method to characterize B. subtilis biofilm morphologies under various growth conditions. To determine biofilm rim irregularities, we used the dimensionless P2A ratio, defined as P2/4πA, where P is the perimeter and A is the area of the biofilm. To estimate biofilm thickness from transmission images, we developed a calibration procedure based on Beer- Lambert’s law and cross sectioning. Furthermore, to determine the distributions of different B. subtilis cell phenotypes during biofilm growth, we used a triple-fluorescence-labeled B. subtilis strain that expressed motility, matrix production, and sporulation. Based on this work, we are able to tune biofilm growth by changing its growing environment.

Keywords: Bacillus subtilis;biofilm;fluorescence;morphology;phenotype

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Morphologies and phenotypes in Bacillus subtilis biofilms

J. Microbiol. 2017;55(8):619-627. Published online July 4, 2017

DOI: https://doi.org/10.1007/s12275-017-7041-z

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