Abstract
The gut bacterial community of wood-feeding beetles has
been examined for its role on plant digestion and biocontrol
method
development. Monochamus alternatus and Psacothea
hilaris, both belonging to the subfamily Lamiinae, are woodfeeding
beetles found in eastern Asia and Europe and generally
considered as destructive pests for pine and mulberry
trees, respectively. However, limited reports exist on the gut
bacterial communities in these species. Here, we characterized
gut bacterial community compositions in larva and imago of
each insect species reared with host tree logs and artificial
diets as food sources. High-throughput 454 pyrosequencing
of bacterial 16S rRNA gene revealed 225 operational taxonomic
units (OTUs) based on a 97% sequences similarity cutoff
from 138,279 sequence reads, the majority of which were
derived from Proteobacteria (48.2%), Firmicutes (45.5%), and
Actinobacteria (5.2%). The OTU network analysis revealed
7 modules with densely connected OTUs in specific gut samples,
in which the distributions of Lactococcus-, Kluyvera-,
Serratia-, and Enterococcus-related OTUs were distinct between
diet types or developmental stages of the host insects.
The gut bacterial communities were separated on a detrended
correspondence analysis (DCA) plot and by c-means fuzzy
clustering analysis, according to diet type. The results from
this study suggest that diet was the main determinant for gut
bacterial community composition in the two beetles.
Citations
Citations to this article as recorded by

- Host genetics and larval host plant modulate microbiome structure and evolution underlying the intimate insect–microbe–plant interactions in Parnassius species on the Qinghai‐Tibet Plateau
Chengyong Su, Tingting Xie, Lijun Jiang, Yunliang Wang, Ying Wang, Ruie Nie, Youjie Zhao, Bo He, Junye Ma, Qun Yang, Jiasheng Hao
Ecology and Evolution.2024;[Epub] CrossRef - Gut bacteria facilitate leaf beetles in adapting to dietary specialization by enhancing larval fitness
Meiqi Ma, Jing Luo, Xiaotong Chen, Chong Li, Siqun Li, Jianghua Sun, Letian Xu
npj Biofilms and Microbiomes.2024;[Epub] CrossRef - Metabolomics and microbiome provide new insights into mechanisms of the variations between the larva and adult stages of the Model Beetle Tribolium castaneum (Herbst)
Jie Bi, Lirui Zhang, Yanping He
Journal of Asia-Pacific Entomology.2024; 27(2): 102241. CrossRef - Enhancing the bioconversion rate and end products of black soldier fly (BSF) treatment – A comprehensive review
Shahida Anusha Siddiqui, Özge Süfer, Gülşah Çalışkan Koç, Hanif Lutuf, Teguh Rahayu, Roberto Castro-Muñoz, Ito Fernando
Environment, Development and Sustainability.2024;[Epub] CrossRef - Substantially altered bacterial diversity associated with developmental stages of litchi stink bug, Tessaratoma javanica (Thunberg) (Hemiptera: Tessaratomidae)
Anita Kumari, Jaipal Singh Choudhary, Anand Kumar Thakur, Sushmita Banra, Priti Kumari Oraon, Kanika Kumari, Subhash Kumar Sahu, Mohammed Fahad Albeshr
Heliyon.2024; 10(11): e32384. CrossRef - Bacterial Diversity in the Intestine of Larvae of the Mesquite Borer Beetle, Placosternus erythropus 1
Orthon R. Vargas-Cardoso, Víctor H. Bustamante, Luz Bretón-Deval, Israel Aguilar-Ordóñez, Jorge Valdez-Carrasco, Víctor H. Toledo-Hernández, César Sotelo-Leyva, Víctor R. Juárez-González
Southwestern Entomologist.2024;[Epub] CrossRef - Unearthing Lactococcus lactis and Scheffersomyeces symbionts from edible wood-boring beetle larvae as a bio-resource for industrial applications
Shadrack Kibet, Cynthia M. Mudalungu, Njogu M. Kimani, JohnMark O. Makwatta, James Kabii, Subramanian Sevgan, Segenet Kelemu, Chrysantus M. Tanga
BMC Microbiology.2024;[Epub] CrossRef - Simbiyotik Fungusların Cerambycid Türler için Önemi
Furkan Doğan, İsmail Oğuz Özdemir, Salih Karabörklü
Journal of Agricultural Biotechnology.2024; 5(2): 79. CrossRef - Risk assessment of predatory lady beetle Propylea japonica's multi-generational exposure to three non-insecticidal agrochemicals
Guofeng Chang, Hui Xue, Jichao Ji, Li Wang, Xiangzhen Zhu, Kaixin Zhang, Dongyang Li, Xueke Gao, Lin Niu, Mengxue Gao, Junyu Luo, Jinjie Cui
Science of The Total Environment.2023; 886: 163931. CrossRef - Gut microbiota assemblages of generalist predators are driven by local- and landscape-scale factors
Hafiz Sohaib Ahmed Saqib, Linyang Sun, Gabor Pozsgai, Pingping Liang, Mohsan Ullah Goraya, Komivi Senyo Akutse, Minsheng You, Geoff M. Gurr, Shijun You
Frontiers in Microbiology.2023;[Epub] CrossRef - Effect of Different Host Plants on the Diversity of Gut Bacterial Communities of Spodoptera frugiperda (J. E. Smith, 1797)
Shipeng Han, Yayuan Zhou, Da Wang, Qiuju Qin, Peng Song, Yunzhuan He
Insects.2023; 14(3): 264. CrossRef - Biotic and abiotic factors affecting the microbiota of Chrysomelidae inhabiting wetland vegetation
Giulia Magoga, Matteo Brunetti, Lukasz Kajtoch, Alberto Spada, Matteo Montagna
Hydrobiologia.2023; 850(17): 3797. CrossRef - Composition and Diversity of Gut Bacterial Community in Different Life Stages of a Leaf Beetle Gastrolina depressa
Meiqi Ma, Xiaotong Chen, Siqun Li, Jing Luo, Runhua Han, Letian Xu
Microbial Ecology.2023; 86(1): 590. CrossRef - Dynamic changes of gut bacterial communities present in larvae of Anoplophora glabripennies collected at different developmental stages
XueFei Wang, HuaLing Wang, XiaoYu Su, Jie Zhang, JiaWei Bai, JianYong Zeng, HuiPing Li
Archives of Insect Biochemistry and Physiology.2023;[Epub] CrossRef - Amendment of a thermophile-fermented compost to humus improves the growth of female larvae of the Hercules beetleDynastes hercules(Coleoptera: scarabaeidae)
Futo Asano, Arisa Tsuboi, Shigeharu Moriya, Tamotsu Kato, Naoko Tsuji, Teruno Nakaguma, Hiroshi Ohno, Hirokuni Miyamoto, Hiroaki Kodama
Journal of Applied Microbiology.2023;[Epub] CrossRef - First Glimpse of Gut Microbiota of Quarantine Insects in China
Yanxue Yu, Qi Wang, Ping Zhou, Na Lv, Wei Li, Fangqing Zhao, Shuifang Zhu, Di Liu
Genomics, Proteomics & Bioinformatics.2022; 20(2): 394. CrossRef - The Comparison of Gut Bacteria Communities and the Functions Among the Sympatric Grasshopper Species From the Loess Plateau
Lu Zhao, Wen-Qiang Wang, Sheng-Quan Xu, De-Long Guan
Frontiers in Microbiology.2022;[Epub] CrossRef - Duplication of Horizontally Acquired GH5_2 Enzymes Played a Central Role in the Evolution of Longhorned Beetles
Na Ra Shin, Daniel Doucet, Yannick Pauchet, Aya Takahashi
Molecular Biology and Evolution.2022;[Epub] CrossRef - Host species identity shapes the diversity and structure of insect microbiota
Antonino Malacrinò
Molecular Ecology.2022; 31(3): 723. CrossRef - Comparison of Gut Bacterial Communities of Locusta migratoria manilensis (Meyen) Reared on Different Food Plants
Qian Wang, Yusheng Liu, Xiangchu Yin
Biology.2022; 11(9): 1347. CrossRef - Investigation of Gut Bacterial Communities of Asian Citrus Psyllid (Diaphorina citri) Reared on Different Host Plants
Lixue Meng, Changxiu Xia, Zhixiong Jin, Hongyu Zhang
Insects.2022; 13(8): 694. CrossRef - Gut Bacterial Diversity and Community Structure of Spodoptera exigua (Lepidoptera: Noctuidae) in the Welsh Onion-producing Areas of North China
Lihong Zhou, Chen Chen, Xingya Wang, Adam C N Wong
Journal of Economic Entomology.2022; 115(4): 1102. CrossRef - Bacterial Communities in the Feces of Laboratory Reared Gampsocleis gratiosa (Orthoptera: Tettigoniidae) across Different Developmental Stages and Sexes
Zhijun Zhou, Huimin Huang, Xuting Che
Insects.2022; 13(4): 361. CrossRef - Integrative Insight into Relationships between Florivorous Thrips Haplothrips leucanthemi and H. niger (Insecta, Thysanoptera)
Agnieszka Kaczmarczyk-Ziemba, Halina Kucharczyk, Marek Kucharczyk, Kinga Kucharska
Insects.2022; 13(3): 279. CrossRef - Gut microbial communities associated with phenotypically divergent populations of the striped stem borer Chilo suppressalis (Walker, 1863)
Haiying Zhong, Juefeng Zhang, Fang Li, Jianming Chen
Scientific Reports.2021;[Epub] CrossRef - Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations
Si-Xun Ge, Feng-Ming Shi, Jia-He Pei, Ze-Hai Hou, Shi-Xiang Zong, Li-Li Ren
Frontiers in Microbiology.2021;[Epub] CrossRef - The phyllosphere microbiome of host trees contributes more than leaf phytochemicals to variation in the Agrilus planipennis Fairmaire gut microbiome structure
Judith Mogouong, Philippe Constant, Pierre Legendre, Claude Guertin
Scientific Reports.2021;[Epub] CrossRef - Gut bacterial communities across 12 Ensifera (Orthoptera) at different feeding habits and its prediction for the insect with contrasting feeding habits
Xiang Zheng, Qidi Zhu, Zhijun Zhou, Fangtong Wu, Lixuan Chen, Qianrong Cao, Fuming Shi, Tushar Kanti Dutta
PLOS ONE.2021; 16(4): e0250675. CrossRef - Host Phylogeny and Diet Shape Gut Microbial Communities Within Bamboo-Feeding Insects
Kuanguan Huang, Jie Wang, Junhao Huang, Shouke Zhang, Alfried P. Vogler, Quanquan Liu, Yongchun Li, Maowei Yang, You Li, Xuguo Zhou
Frontiers in Microbiology.2021;[Epub] CrossRef - Gut Bacterial Diversity in Different Life Cycle Stages of Adelphocoris suturalis (Hemiptera: Miridae)
Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Changcai Wu, Xueke Gao, Junyu Luo, Jinjie Cui
Frontiers in Microbiology.2021;[Epub] CrossRef - Gamma radiation effect on the midgut bacteria of Plodia interpunctella and its role in organic wastes biodegradation
Eman A. Mahmoud, Ola E. A. Al-Hagar, Mona F. Abd El-Aziz
International Journal of Tropical Insect Science.2021; 41(1): 261. CrossRef - Host-Plant Induced Shifts in Microbial Community Structure in Small Brown Planthopper, Laodelphax striatellus (Homoptera: Delphacidae)
Hai-Bo Pan, Mu-Yu Li, Wei Wu, Zheng-Liang Wang, Xiao-Ping Yu, Scott Geib
Journal of Economic Entomology.2021; 114(2): 937. CrossRef - Microbiomes of willow-galling sawflies: effects of host plant, gall type, and phylogeny on community structure and function
Craig T. Michell, Tommi Nyman
Genome.2021; 64(6): 615. CrossRef - First Insight into Microbiome Profiles of Myrmecophilous Beetles and Their Host, Red Wood Ant Formica polyctena (Hymenoptera: Formicidae)—A Case Study
Agnieszka Kaczmarczyk-Ziemba, Mirosław Zagaja, Grzegorz K. Wagner, Ewa Pietrykowska-Tudruj, Bernard Staniec
Insects.2020; 11(2): 134. CrossRef - Changes on the intestinal bacterial community of white shrimp Penaeus vannamei fed with green seaweeds
Regina Elizondo-González, Eduardo Quiroz-Guzmán, Adina Howe, Fan Yang, Jared Flater, Maxence Gemin, Elena Palacios, Alberto Peña-Rodríguez
Journal of Applied Phycology.2020; 32(3): 2061. CrossRef - Glyphosate-based herbicide affects the composition of microbes associated with Colorado potato beetle (Leptinotarsa decemlineata)
Carlos Gómez-Gallego, Miia J Rainio, M Carmen Collado, Anastasia Mantziari, Seppo Salminen, Kari Saikkonen, Marjo Helander
FEMS Microbiology Letters.2020;[Epub] CrossRef - The role of the gut microbiome in mediating standard metabolic rate after dietary shifts in the viviparous cockroach,Diploptera punctata
Paul A. Ayayee, George Kinney, Chris Yarnes, Thomas Larsen, Gordon F. Custer, Linda T. A. van Diepen, Agustí Muñoz-Garcia
Journal of Experimental Biology.2020;[Epub] CrossRef - Gut Bacteria in the Holometabola: A Review of Obligate and Facultative Symbionts
R A Kucuk, Oliver Martin
Journal of Insect Science.2020;[Epub] CrossRef - Gut compartments and ovary bacterial symbionts of the Sunn pest
Azam Amiri, Ali R. Bandani, Maryam Kafil
Journal of Asia-Pacific Entomology.2020; 23(3): 723. CrossRef - Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblings Heliconius erato phyllis caterpillars
Rosana Huff, Rebeca Inhoque Pereira, Caroline Pissetti, Aldo Mellender de Araújo, Pedro Alves d’Azevedo, Jeverson Frazzon, Ana Paula GuedesFrazzon
PeerJ.2020; 8: e8647. CrossRef - Effects of Different Hosts on Bacterial Communities of Parasitic Wasp Nasonia vitripennis
Ruxin Duan, Heng Xu, Shanshan Gao, Zheng Gao, Ningxin Wang
Frontiers in Microbiology.2020;[Epub] CrossRef - Bacterial Communities Associated with the Pine Wilt Disease Insect Vector Monochamus alternatus (Coleoptera: Cerambycidae) during the Larvae and Pupae Stages
Hongjian Chen, Dejun Hao, Zhiqiang Wei, Lujie Wang, Tao Lin
Insects.2020; 11(6): 376. CrossRef - Divergence in Gut Bacterial Community Among Life Stages of the Rainbow Stag Beetle Phalacrognathus muelleri (Coleptera: Lucanidae)
Miaomiao Wang, Xingjia Xiang, Xia Wan
Insects.2020; 11(10): 719. CrossRef - The effects of taxonomy, diet, and ecology on the microbiota of riverine macroinvertebrates
Shawn A. Kroetsch, Karen A. Kidd, Wendy A. Monk, Joseph M. Culp, Zacchaeus G. Compson, Scott A. Pavey
Ecology and Evolution.2020; 10(24): 14000. CrossRef - The microbiome profiling of fungivorous black tinder fungus beetleBolitophagus reticulatusreveals the insight into bacterial communities associated with larvae and adults
Agnieszka Kaczmarczyk-Ziemba, Grzegorz K. Wagner, Krzysztof Grzywnowicz, Marek Kucharczyk, Sylwia Zielińska
PeerJ.2019; 7: e6852. CrossRef - Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)
Suresh J. Gawande, Sivalingam Anandhan, Ashish Ingle, Praveen Roylawar, Kiran Khandagale, Tushar Gawai, Alana Jacobson, Ramasamy Asokan, Major Singh, Ulrich Melcher
PLOS ONE.2019; 14(9): e0223281. CrossRef - Fungal, Bacterial, and Archaeal Diversity in the Digestive Tract of Several Beetle Larvae (Coleoptera)
Elvira E. Ziganshina, Waleed S. Mohammed, Elena I. Shagimardanova, Petr Y. Vankov, Natalia E. Gogoleva, Ayrat M. Ziganshin
BioMed Research International.2018; 2018: 1. CrossRef - Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor
Amandine Gales, Lucile Chatellard, Maider Abadie, Anaïs Bonnafous, Lucas Auer, Hélène Carrère, Jean-Jacques Godon, Guillermina Hernandez-Raquet, Claire Dumas
Frontiers in Microbiology.2018;[Epub] CrossRef - First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis
Agnieszka Kaczmarczyk, Halina Kucharczyk, Marek Kucharczyk, Przemysław Kapusta, Jerzy Sell, Sylwia Zielińska
Scientific Reports.2018;[Epub] CrossRef - Different laboratory populations similar bacterial profile? The case of Glossina palpalis gambiensis
Vangelis Doudoumis, Antonios Augustinos, Aggeliki Saridaki, Andrew Parker, Adly M M Abd-Alla, Kostas Bourtzis, George Tsiamis
BMC Microbiology.2018;[Epub] CrossRef - Impact of Rearing Conditions on the Ambrosia Beetle’s Microbiome
Luis Arturo Ibarra-Juarez, Damaris Desgarennes, Mirna Vázquez-Rosas-Landa, Emanuel Villafan, Alexandro Alonso-Sánchez, Ofelia Ferrera-Rodríguez, Andrés Moya, Daniel Carrillo, Luisa Cruz, Gloria Carrión, Abel López-Buenfil, Clemente García-Avila, Enrique I
Life.2018; 8(4): 63. CrossRef - Dynamic Effects of Initial pH of Substrate on Biological Growth and Metamorphosis of Black Soldier Fly (Diptera: Stratiomyidae)
Junhua Ma, Yanyan Lei, Kashif ur Rehman, Ziniu Yu, Jibin Zhang, Wu Li, Qing Li, Jeffery K Tomberlin, Longyu Zheng
Environmental Entomology.2018; 47(1): 159. CrossRef - Insect biorefinery: a green approach for conversion of crop residues into biodiesel and protein
Hui Wang, Kashif ur Rehman, Xiu Liu, Qinqin Yang, Longyu Zheng, Wu Li, Minmin Cai, Qing Li, Jibin Zhang, Ziniu Yu
Biotechnology for Biofuels.2017;[Epub] CrossRef