Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli

Su-Mi Choi; Jae-Ho Jeong; Hyon E. Choy; Minsang Shin

doi:10.1007/s12275-016-6027-6

Journal Article
Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli: Su-Mi Choi ¹, Jae-Ho Jeong ¹, Hyon E. Choy ¹, Minsang Shin ²; Journal of Microbiology 2016;54(8):559-564.
DOI: https://doi.org/10.1007/s12275-016-6027-6
Published online: August 2, 2016

¹Department of Microbiology, Chonnam National University Medical School, Gwangju 61186, Republic of Korea, ²Department of Microbiology, Kyungpook National University School of Medicine, Daegu 41944, Republic of Korea¹Department of Microbiology, Chonnam National University Medical School, Gwangju 61186, Republic of Korea, ²Department of Microbiology, Kyungpook National University School of Medicine, Daegu 41944, Republic of Korea

Corresponding author: Hyon E. Choy , Tel: +82-53-420-4841,
Minsang Shin , Tel: +82-53-420-4841,

Received: 21 January 2016 • Revised: 30 May 2016 • Accepted: 16 June 2016

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Abstract

Enteropathogenic E. coli causes attaching and effacing (A/E) intestinal lesions. The genes involved in the formation of A/E lesions are encoded within a chromosomal island comprising of five major operons, LEE1-5. The global regulator H-NS represses the expression of these operons. Ler, a H-NS homologue, counteracts the H-NS–mediated repression. Using a novel genetic approach, we identified the amino acid residues in Ler that are involved in the interaction with H-NS: I20 and L23 in the C-terminal portion of α-helix 3, and I42 in the following unstructured linker region.

Keywords: enteropathogenic E. coli (EPEC);H-NS;LEE;Ler;transcription repression

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Regulation of the Locus of Enterocyte Effacement in Attaching and Effacing Pathogens
R. Christopher D. Furniss, Abigail Clements, William Margolin
Journal of Bacteriology.2018;[Epub] CrossRef

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Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli

J. Microbiol. 2016;54(8):559-564. Published online August 2, 2016

DOI: https://doi.org/10.1007/s12275-016-6027-6

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