Dimethyl sulfoxide reduction by a hyperhermophilic archaeon Thermococcus onnurineus NA1 via a cysteine-cystine redox shuttle

Ae Ran Choi; Min-Sik Kim; Sung Gyun Kang; Hyun Sook Lee

doi:10.1007/s12275-016-5574-1

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Dimethyl sulfoxide reduction by a hyperhermophilic archaeon Thermococcus onnurineus NA1 via a cysteine-cystine redox shuttle: Ae Ran Choi ¹, Min-Sik Kim ¹, Sung Gyun Kang ^1,2, Hyun Sook Lee ^1,2; Journal of Microbiology 2016;54(1):31-38.
DOI: https://doi.org/10.1007/s12275-016-5574-1
Published online: January 5, 2016

¹Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea, ²Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea¹Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea, ²Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea

Corresponding author: Hyun Sook Lee , Tel: +82-31-400-6248,

Received: 19 November 2015 • Accepted: 3 December 2015

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Abstract

A variety of microbes grow by respiration with dimethyl sulfoxide (DMSO) as an electron acceptor, and several distinct DMSO respiratory systems, consisting of electron carriers and a terminal DMSO reductase, have been characterized. The heterotrophic growth of a hyperthermophilic archaeon Thermococcus onnurineus NA1 was enhanced by the addition of DMSO, but the archaeon was not capable of reducing DMSO to DMS directly using a DMSO reductase. Instead, the archaeon reduced DMSO via a cysteine-cystine redox shuttle through a mechanism whereby cystine is microbially reduced to cysteine, which is then reoxidized by DMSO reduction. A thioredoxin reductase-protein disulfide oxidoreductase redox couple was identified to have intracellular cystine-reducing activity, permitting recycle of cysteine. This study presents the first example of DMSO reduction via an electron shuttle. Several Thermococcales species also exhibited enhanced growth coupled with DMSO reduction, probably by disposing of excess reducing power rather than conserving energy.

Keywords: DMSO;Thermococcus onnurineus NA1;cysteinecystine redox shuttle;thioredoxin reductase;protein disulfide oxidoreductase;extracellular electron mediator

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Dimethyl sulfoxide reduction by a hyperhermophilic archaeon Thermococcus onnurineus NA1 via a cysteine-cystine redox shuttle

J. Microbiol. 2016;54(1):31-38. Published online January 5, 2016

DOI: https://doi.org/10.1007/s12275-016-5574-1

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