Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation

May Nyan Taw; Hae-In Lee; Sang-Ho Lee; Woo-Suk Chang

doi:10.1007/s12275-015-5313-z

Articles

Page Path: HOME > J. Microbiol > Volume 53(8); 2015 > Article

Journal Article
Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation: May Nyan Taw ¹, Hae-In Lee ¹, Sang-Ho Lee ², Woo-Suk Chang ^1,3; Journal of Microbiology 2015;53(8):518-525.
DOI: https://doi.org/10.1007/s12275-015-5313-z
Published online: July 31, 2015

¹Department of Biology, University of Texas at Arlington, Arlington, Texas 76019, USA, ²Fusion Technology R&D Division, Korea Institute of Industrial Technology, Ansan 426-910, Republic of Korea, ³Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Republic of Korea¹Department of Biology, University of Texas at Arlington, Arlington, Texas 76019, USA, ²Fusion Technology R&D Division, Korea Institute of Industrial Technology, Ansan 426-910, Republic of Korea, ³Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Republic of Korea

Corresponding author: Woo-Suk Chang , Tel: +1-817-272-3280,

Received: 19 June 2015 • Revised: 15 July 2015 • Accepted: 20 July 2015

prev next

50 Views
0 Download
10 Crossref
9 Scopus

Full Article

Abstract

Bradyrhizobium japonicum is a Gram-negative soil bacterium that can fix nitrogen into ammonia by developing a symbiotic relationship with the soybean plant. MocR proteins make up a subfamily of GntR superfamily, one of the most widely distributed and prolific groups of the helix-turn-helix transcription factors. In this study, we constructed a mutant strain for mocR (blr6977) to investigate its role in cellular processes and symbiosis in B. japonicum. Although growth rate and morphology of the mutant were indistinguishable from those of the wild type, the mutant showed significant differences in motility and attachment (i.e., biofilm formation) from the wild type. The mutant displayed a decrease in biofilm formation, but was more motile than the wild type. The inactivation of mocR did not affect the number of nodules on soybean roots, but caused delayed nodulation. Delayed nodulation intrigued us to study competitiveness of the mutant infecting soybeans. The mutant was less competitive than the wild type, indicating that delayed nodulation might be due to competitiveness. Gene expressions of other MocR subfamily members were also compared between the wild type and mutant strains. None of the mocR-like genes examined in this study were differentially expressed between both strains.

Keywords: GntR transcriptional regulator;MocR;Bradyrhizobium japonicum;motility;biofilm;symbiotic nitrogen fixation

Figure & Data

References

Citations

Citations to this article as recorded by

Antibiofilm efficacies and mechanism of perillaldehyde against Shewanella putrefaciens
Wenxiu Zhu, Yuanhang Cheng, Yankun Zhang, Mingxin Li, Yue Teng, Yunqi Gu, Haisong Wang, Xiaodong Xia
Food Microbiology.2025; 128: 104699. CrossRef
Prevalence and virulence of Vibrio parahaemolyticus isolated from clinical and environmental samples in Huzhou, China
Peng Zhang, Xiaofang Wu, Lei Ji, Wei Yan, Liping Chen, Zhonghao Lu, Deshun Xu, Yunfeng Zha, Dafang Xu, Fenfen Dong
BMC Genomics.2024;[Epub] CrossRef
The MocR family transcriptional regulator DnfR has multiple binding sites and regulates Dirammox gene transcription in Alcaligenes faecalis JQ135
Si‐Qiong Xu, Xiao Wang, Lu Xu, Ke‐Xin Wang, Yin‐Hu Jiang, Fu‐Yin Zhang, Qing Hong, Jian He, Shuang‐Jiang Liu, Ji‐Guo Qiu
Environmental Microbiology.2023; 25(3): 675. CrossRef
The GntR-like transcriptional regulator HutC involved in motility, biofilm-forming ability, and virulence in Vibrio parahaemolyticus
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Microbial Pathogenesis.2022; 167: 105546. CrossRef
Biological and transcriptional studies reveal VmeL is involved in motility, biofilm formation and virulence in Vibrio parahaemolyticus
Peng-xuan Liu, Xiao-yun Zhang, Quan Wang, Yang-yang Li, Wei-dong Sun, Yu Qi, Kai Zhou, Xian-gan Han, Zhao-guo Chen, Wei-huan Fang, Wei Jiang
Frontiers in Microbiology.2022;[Epub] CrossRef
PrbP modulates biofilm formation in Liberibacter crescens
Lei Pan, Christopher L. Gardner, Reagan Beliakoff, Danilo da Silva, Ran Zuo, Fernando A. Pagliai, Kaylie A. Padgett‐Pagliai, Marcelo L. Merli, Erol Bahadiroglu, Claudio F. Gonzalez, Graciela L. Lorca
Environmental Microbiology.2021; 23(11): 7121. CrossRef
The glucose uptake systems in Corynebacterium glutamicum: a review
Haozhe Ruan, Haibo Yu, Jianzhong Xu
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef
Comparative Genomics of Pseudomonas sp. Strain SI-3 Associated With Macroalga Ulva prolifera, the Causative Species for Green Tide in the Yellow Sea
Huihui Fu, Peng Jiang, Jin Zhao, Chunhui Wu
Frontiers in Microbiology.2018;[Epub] CrossRef
The MocR‐like transcription factors: pyridoxal 5′‐phosphate‐dependent regulators of bacterial metabolism
Angela Tramonti, Caterina Nardella, Martino L. di Salvo, Stefano Pascarella, Roberto Contestabile
The FEBS Journal.2018; 285(21): 3925. CrossRef
Negative Regulation of Ectoine Uptake and Catabolism in Sinorhizobium meliloti: Characterization of the EhuR Gene
Qinli Yu, Hanlin Cai, Yanfeng Zhang, Yongzhi He, Lincai Chen, Justin Merritt, Shan Zhang, Zhiyang Dong, Anke Becker
Journal of Bacteriology.2017;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation

J. Microbiol. 2015;53(8):518-525. Published online July 31, 2015

DOI: https://doi.org/10.1007/s12275-015-5313-z

Related articles