Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription

Hyung-Ju Lim; Kwangsoo Kim; Minsang Shin; Jae-Ho Jeong; Phil Youl Ryu; Hyon E. Choy

doi:10.1007/s12275-015-4681-8

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Research Support, Non-U.S. Gov't
Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription: Hyung-Ju Lim , Kwangsoo Kim , Minsang Shin , Jae-Ho Jeong , Phil Youl Ryu , Hyon E. Choy; Journal of Microbiology 2015;53(4):250-255.
DOI: https://doi.org/10.1007/s12275-015-4681-8
Published online: April 8, 2015

Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of KoreaDepartment of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea

Corresponding author: Hyon E. Choy , Tel: +82-62-220-4137,

Received: 3 December 2014 • Revised: 15 January 2015 • Accepted: 26 January 2015

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Abstract

σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone- like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The
results
suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

Keywords: E. coli;promoter specificity;RNA polymerase;rpoS;σ38

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Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription

J. Microbiol. 2015;53(4):250-255. Published online April 8, 2015

DOI: https://doi.org/10.1007/s12275-015-4681-8

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