Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics

Jae Yoon Hwang; Soo Hee Kim; Hye Ryeung Oh; Eunju Kwon; Doo Hyun Nam

doi:10.1007/s12275-015-4340-0

Articles

Page Path: HOME > J. Microbiol > Volume 53(1); 2015 > Article

Research Support, Non-U.S. Gov't
Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics: Jae Yoon Hwang ¹, Soo Hee Kim ¹, Hye Ryeung Oh ¹, Eunju Kwon ², Doo Hyun Nam ¹; Journal of Microbiology 2015;53(1):84-89.
DOI: https://doi.org/10.1007/s12275-015-4340-0
Published online: December 4, 2014

¹College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea, ²Institute for Drug Development, Yeungnam University, Gyongsan 712-749, Republic of Korea¹College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea, ²Institute for Drug Development, Yeungnam University, Gyongsan 712-749, Republic of Korea

Corresponding author: Doo Hyun Nam , Tel: +82-53-810-2825,

Received: 13 June 2014 • Revised: 31 July 2014 • Accepted: 20 August 2014

prev next

66 Views
0 Download
7 Crossref
9 Scopus

Full Article

Abstract

Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains 8.04 Mb with 73.6% G+C content and 7,810 open reading frames. All the genes for aerial mycelium and spore formations were confirmed in this draft genome. In phylogenetic analysis of MurE proteins (UDPN- acetylmuramyl-L-alanyl-D-glutamate:DAP ligase) in a conserved dcw (division of cell wall) locus, MurE proteins of Kitasatospora species were placed in a separate clade between MurEs of Streptomyces species incorporating LL-diaminopimelic acid (DAP) and MurEs of Saccharopolyspora erythraea as well as Mycobacterium tuberculosis ligating meso- DAP. From this finding, it was assumed that Kitasatospora MurEs exhibit the substrate specificity for both LL-DAP and meso-DAP. The bafilomycin biosynthetic gene cluster was located in the left subtelomeric region. In 71.3 kb-long gene cluster, 17 genes probably involved in the biosynthesis of bafilomycin derivatives were deduced, including 5 polyketide synthase (PKS) genes comprised of 12 PKS modules.

Keywords: draft genome;Kitasatospora;MurE;polyketide synthase;bafilomycin

Figure & Data

References

Citations

Citations to this article as recorded by

Genome-directed discovery of antiproliferative bafilomycins from a deepsea-derived Streptomyces samsunensis
Runyi Wang, Yilei Bao, Yujing Dong, Yun Dong, Huayue Li
Bioorganic Chemistry.2023; 138: 106599. CrossRef
Engineering Bafilomycin High-Producers by Manipulating Regulatory and Biosynthetic Genes in the Marine Bacterium Streptomyces lohii
Zhong Li, Shuai Li, Lei Du, Xingwang Zhang, Yuanyuan Jiang, Wenhua Liu, Wei Zhang, Shengying Li
Marine Drugs.2021; 19(1): 29. CrossRef
Genome based characterization of Kitasatospora sp. MMS16-BH015, a multiple heavy metal resistant soil actinobacterium with high antimicrobial potential
Bo-Ram Yun, Adeel Malik, Seung Bum Kim
Gene.2020; 733: 144379. CrossRef
Genome Mining of the Genus Streptacidiphilus for Biosynthetic and Biodegradation Potential
Adeel Malik, Yu Ri Kim, Seung Bum Kim
Genes.2020; 11(10): 1166. CrossRef
Biosynthesis of 2-amino-3-hydroxycyclopent-2-enone moiety of bafilomycin in Kitasatospora cheerisanensis KCTC2395
Nguyen Phan Kieu Hanh, Jae Yoon Hwang, Hye Ryeung Oh, Geum Jin Kim, Hyukjae Choi, Doo Hyun Nam
Journal of Microbiology.2018; 56(8): 571. CrossRef
Genus Kitasatospora, taxonomic features and diversity of secondary metabolites
Yōko Takahashi
The Journal of Antibiotics.2017; 70(5): 506. CrossRef
Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques
Kateřina Petříčková, Alica Chroňáková, Tomáš Zelenka, Tomáš Chrudimský, Stanislav Pospíšil, Miroslav Petříček, Václav Krištůfek
Frontiers in Microbiology.2015;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics

J. Microbiol. 2015;53(1):84-89. Published online December 4, 2014

DOI: https://doi.org/10.1007/s12275-015-4340-0

Related articles