σB Affects Biofilm Formation under the Dual Stress Conditions Imposed by Adding Salt and Low Temperatur in Listeria monocytogenes

Jin-Ju Lee; Gilho Lee; Ji-Hyun Shin

doi:10.1007/s12275-014-4369-5

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Research Support, Non-U.S. Gov't
σB Affects Biofilm Formation under the Dual Stress Conditions Imposed by Adding Salt and Low Temperatur in Listeria monocytogenes: Jin-Ju Lee ¹, Gilho Lee ², Ji-Hyun Shin ¹; Journal of Microbiology 2014;52(10):849-855.
DOI: https://doi.org/10.1007/s12275-014-4369-5
Published online: October 1, 2014

¹Institute of Science and Technology, College of Science and Technology, Korea University, Sejong 339-700, Republic of Korea, ²Department of Urology, Dankook University College of Medicine, Cheonan 330-715, Republic of Korea¹Institute of Science and Technology, College of Science and Technology, Korea University, Sejong 339-700, Republic of Korea, ²Department of Urology, Dankook University College of Medicine, Cheonan 330-715, Republic of Korea

Corresponding author: Ji-Hyun Shin , Tel: +82-44-860-1912,

Received: 25 June 2014 • Revised: 19 August 2014 • Accepted: 26 August 2014

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Abstract

The food-borne pathogenic bacteria Listeria monocytogenes can form biofilms on various surfaces including food-processing equipment. Biofilms offer survival benefits to the organisms entrapped against environmental insults. Moreover, the σB transcription factor of L. monocytogenes plays an important role in its survival under various stress conditions. In this study, we evaluated whether σB contributes to biofilm formation when L. monocytogenes is grown under various temperatures and media. When the wild-type strain was grown under static biofilm culture below ambient temperature (15°C) for 72 h, the difference in viable cell number (in both planktonic and biofilm cells) between the wild-type and ΔsigB mutant increased by adding NaCl to BHI broth (9% salt BHI > 6% salt BHI > BHI, w/v), and the specific activity of β-galactosidase was highly induced in the wild-type strain grown in 6% salt containing BHI broth. Furthermore, we measured surface-adhered biofilm forming ability using the crystal violet staining method. The wild-type strain formed a four times larger biofilm than that of the ΔsigB mutant in 6% salt-BHI medium at 15°C over a 72 h incubation and also showed the highest level of β-galactosidase specific activity. However, both the wild-type and ΔsigB mutant L. monocytogenes were defective for forming a biofilm in 9% salt-BHI medium at 15°C. Our results suggest that σB plays an enhanced role in surface-adhered biofilm formation when L. monocytogenes encounters dual stress conditions, such as 6% NaCl and low temperature.

Keywords: Listeria monocytogenes;biofilm;σB;low temperature;salt stress

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σB Affects Biofilm Formation under the Dual Stress Conditions Imposed by Adding Salt and Low Temperatur in Listeria monocytogenes

J. Microbiol. 2014;52(10):849-855. Published online October 1, 2014

DOI: https://doi.org/10.1007/s12275-014-4369-5

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