Pseudomonas aeruginosa MdaB and WrbA are Water-soluble Two-electron Quinone Oxidoreductases with the Potential to Defend against Oxidative Stress

Laura K Green; Anne C La Flamme; David F Ackerley

doi:10.1007/s12275-014-4208-8

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Pseudomonas aeruginosa MdaB and WrbA are Water-soluble Two-electron Quinone Oxidoreductases with the Potential to Defend against Oxidative Stress: Laura K Green ^1,2, Anne C La Flamme ^1,2,3, David F Ackerley ^1,2,3; Journal of Microbiology 2014;52(9):771-777.
DOI: https://doi.org/10.1007/s12275-014-4208-8
Published online: August 2, 2014

¹School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand, ²Centre for Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand, ³Maurice Wilkins Centre for Molecular Biodiscovery, School of Biological Sciences, University of Auckland, Auckland 1010, New Zealand¹School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand, ²Centre for Biodiscovery, Victoria University of Wellington, Wellington 6012, New Zealand, ³Maurice Wilkins Centre for Molecular Biodiscovery, School of Biological Sciences, University of Auckland, Auckland 1010, New Zealand

Corresponding author: David F Ackerley , Tel: +64-4-4635576,

Received: 3 April 2014 • Revised: 25 June 2014 • Accepted: 2 July 2014

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Abstract

Water-soluble quinone oxidoreductases capable of reducing quinone substrates via a concerted two-electron mechanism have been implicated in bacterial antioxidant defence. Twoelectron transfer avoids formation of dangerously reactive semi-quinone intermediates, moreover previous work in Pseudomonas putida indicated a direct protective effect for the quinols generated by an over-expressed oxidoreductase. Here, the Pseudomonas aeruginosa orthologs of five quinone oxidoreductases – MdaB, ChrR, WrbA, NfsB, and NQO1 – were tested for their possible role in defending P. aeruginosa against H2O2 challenge. In in vitro assays, each enzyme was shown to reduce quinone substrates with only minimal semiquinone formation. However, when each was individually over-expressed in P. aeruginosa no overt H2O2-protective phenotype was observed. It was shown that this was due to a masking effect of the P. aeruginosa catalase, KatA; in a katA mutant, H2O2 challenged strains over-expressing the WrbA and MdaB orthologs grew significantly better than the empty plasmid control. A growth advantage was also observed for H2O2 challenged P. putida strains over-expressing P. aeruginosa wrbA, mdaB or katA. Despite not conferring a growth advantage to wild type P. aeruginosa, it is possible that these quinone oxidoreductases defend against H2O2 toxicity at lower concentrations.

Keywords: NAD(P)H dependent oxidoreductase;quinone reductase;hydrogen peroxide;KatA;Pseudomonas putida

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Pseudomonas aeruginosa MdaB and WrbA are Water-soluble Two-electron Quinone Oxidoreductases with the Potential to Defend against Oxidative Stress

J. Microbiol. 2014;52(9):771-777. Published online August 2, 2014

DOI: https://doi.org/10.1007/s12275-014-4208-8

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