Antimicrobial Resistance, Virulence Genes and PFGE-profiling of Escherichia coli Isolates from South Korean Cattle Farms

Seung Won Shin; Jae-Won Byun; Myounghwan Jung; Min-Kyoung Shin; Han Sang Yoo

doi:10.1007/s12275-014-4166-1

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Antimicrobial Resistance, Virulence Genes and PFGE-profiling of Escherichia coli Isolates from South Korean Cattle Farms: Seung Won Shin ¹, Jae-Won Byun ², Myounghwan Jung ¹, Min-Kyoung Shin ¹, Han Sang Yoo ^1,3; Journal of Microbiology 2014;52(9):785-793.
DOI: https://doi.org/10.1007/s12275-014-4166-1
Published online: July 30, 2014

¹Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea, ²Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea, ³Institute of Green Bio Science and Technology, Seoul National University, Pyeungchang 232-916, Republic of Korea¹Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea, ²Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea, ³Institute of Green Bio Science and Technology, Seoul National University, Pyeungchang 232-916, Republic of Korea

Corresponding author: Han Sang Yoo , Tel: +82-2-880-1263,

Received: 18 March 2014 • Revised: 18 June 2014 • Accepted: 18 June 2014

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Abstract

To estimate the prevalence of Escherichia coli with potential pathogenicity in cattle farm in South Korea, a total of 290 E. coli isolates were isolated from cattle farms over a period of 2 years in South Korea. These were examined for phenotypic and genotypic characteristics including antimicrobial susceptibility, serotype, and gene profiles of virulence and antimicrobial resistance. The most dominant virulence gene was f17 (26.2%), followed by stx2 (15.9%), ehxA (11.0%), stx1 (8.3%), eae (5.2%), and sta (4.1%). Some shiga-toxin producing E. coli isolates possessed eae (15.9%). All isolates except for one showed resistance to one or more antimicrobials, with 152 isolates exhibiting multidrug-resistance. The most prevalent resistance phenotype detected was streptomycin (63.1%), followed by tetracycline (54.5%), neomycin (40.3%), cephalothin (32.8%), amoxicillin (30.0%), ampicillin (29.7%), and sulphamethoxazole/trimethoprim (16.6%). The associated resistance determinants detected were strAstrB (39.0%), tet(E) (80.0%), tet(A) (27.6%), aac(3)-IV (33.1%), aphA1 (21.4%), blaTEM (23.8%), and sul2 (22.1%). When investigated by O serotyping and PFGE molecular subtyping, the high degree of diversity was exhibited in E. coli isolates. These results suggest that E. coli isolates from South Korean cattle farms are significantly diverse in terms of virulence and antimicrobial resistance. In conclusion, the gastroinstestinal flora of cattle could be a significant reservoir of diverse virulence and antimicrobial resistance determinants, which is potentially hazardous to public health.

Keywords: Escherichia coli;prevalence;virulence;antimicrobial resistance;serotyping;PFGE

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Antimicrobial Resistance, Virulence Genes and PFGE-profiling of Escherichia coli Isolates from South Korean Cattle Farms

J. Microbiol. 2014;52(9):785-793. Published online July 30, 2014

DOI: https://doi.org/10.1007/s12275-014-4166-1

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