Growth Phase-dependent Roles of Sir2 in Oxidative Stress Resistance and Chronological Lifespan in Yeast

Woo Kyu Kang; Yeong Hyeock Kim; Byoung-Soo Kim; Jeong-Yoon Kim

doi:10.1007/s12275-014-4173-2

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Growth Phase-dependent Roles of Sir2 in Oxidative Stress Resistance and Chronological Lifespan in Yeast: Woo Kyu Kang ¹, Yeong Hyeock Kim ¹, Byoung-Soo Kim ², Jeong-Yoon Kim ¹; Journal of Microbiology 2014;52(8):652-658.
DOI: https://doi.org/10.1007/s12275-014-4173-2
Published online: July 5, 2014

¹Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Department of Physiology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea¹Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Department of Physiology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea

Corresponding author: Jeong-Yoon Kim , Tel: +82-42-821-6419,

Received: 20 March 2014 • Revised: 7 May 2014 • Accepted: 7 May 2014

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Abstract

Silent Information Regulator 2 (Sir2), a conserved NAD+- dependent histone deacetylase, has been implicated as one of the key factors in regulating stress response and longevity. Here, we report that the role of Sir2 in oxidative stress resistance and chronological lifespan is dependent on growth phase in yeast. In exponential phase, sir2Δ cells were more resistant to H2O2 stress and had a longer chronological lifespan than wild type. By contrast, in post-diauxic phase, sir2Δ cells were less resistant to H2O2 stress and had a shorter chronological lifespan than wild type cells. Similarly, the expression of antioxidant genes, which are essential to cope with oxidative stress, was regulated by Sir2 in a growth phasedependent manner. Collectively, our findings highlight the importance of the metabolic state of the cell in determining whether Sir2 can protect against or accelerate cellular aging of yeast.

Keywords: Sir2;oxidative stress resistance;chronological lifespan;aging;Saccharomyces cerevisiae

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Growth Phase-dependent Roles of Sir2 in Oxidative Stress Resistance and Chronological Lifespan in Yeast

J. Microbiol. 2014;52(8):652-658. Published online July 5, 2014

DOI: https://doi.org/10.1007/s12275-014-4173-2

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