Variations of SSU rDNA Group I Introns in Different Isolates of Cordyceps militaris and the Loss of an Intron during Cross-Mating

Tiantian Lian; Tao Yang; Junde Sun; Suping Guo; Huaijun Yang; Caihong Dong

doi:10.1007/s12275-014-3681-4

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Variations of SSU rDNA Group I Introns in Different Isolates of Cordyceps militaris and the Loss of an Intron during Cross-Mating: Tiantian Lian ^1,2, Tao Yang ¹, Junde Sun ², Suping Guo ³, Huaijun Yang ³, Caihong Dong ¹; Journal of Microbiology 2014;52(8):659-666.
DOI: https://doi.org/10.1007/s12275-014-3681-4
Published online: July 4, 2014

¹State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China, ²College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, P. R. China, ³Shanxi Research Institute for Medicine and Life Science, Taiyuan, 030006, P. R. China¹State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China, ²College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, P. R. China, ³Shanxi Research Institute for Medicine and Life Science, Taiyuan, 030006, P. R. China

Corresponding author: Caihong Dong , Tel: +86-010-64806138,

Received: 26 December 2013 • Revised: 11 April 2014 • Accepted: 12 May 2014

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Abstract

Cordyceps militaris, the type species of genus Cordyceps, is one of the most popular mushrooms and a nutraceutical in eastern Asia. It is considered a model organism for the study of Cordyceps species because it can complete its life cycle when cultured in vitro. In the present study, the occurrence and sequence variation of SSU rDNA group I introns, Cmi.S943 and Cmi.S1199, among different isolates of C. militaris were analyzed. Based on the secondary structure predictions, the Cmi.S943 intron has been placed in subgroup IC1, and the Cmi.S1199 intron has been placed in subgroup IE. No significant similarity between Cmi.S943 and Cmi.S1199 suggested different origins. Three genotypes, based on the frequency and distribution of introns, were described to discriminate the 57 surveyed C. militaris strains. It was found that the genotype was related to the stroma characteristics. The stromata of all of the genotype II strains, which possessed only Cmi.S943, could produce perithecium. In contrast, the stromata of all genotype III strains, which had both Cmi.S943 and Cmi.S1199, could not produce perithecium. Cmi.S1199 showed the lowest level of intra-specific variation among the tested strains. Group I introns can be lost during strain cross-mating. Therefore, we presumed that during cross-mating and recombination, intron loss could be driven by positive Darwinian selection due to the energetic cost of transcribing long introns.

Keywords: Cordyceps militaris;group I introns;occurrence;sequence variation;stroma characteristics

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Variations of SSU rDNA Group I Introns in Different Isolates of Cordyceps militaris and the Loss of an Intron during Cross-Mating

J. Microbiol. 2014;52(8):659-666. Published online July 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3681-4

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