Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque

Ahmed N Aljarbou; Mohamad Aljofan

doi:10.1007/s12275-014-3380-1

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Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque: Ahmed N Aljarbou ¹, Mohamad Aljofan ^1,2; Journal of Microbiology 2014;52(7):609-618.
DOI: https://doi.org/10.1007/s12275-014-3380-1
Published online: May 30, 2014

¹Department of Pharmaceutics, College of Pharmacy, Qassim University, Saudi Arabia, ²Department of Microbiology, School of Medicine and Health Sciences, Monash University, 3600, Australia

Corresponding author: Ahmed N Aljarbou , Tel: .,

Received: 17 July 2013 • Revised: 3 March 2014 • Accepted: 12 March 2014

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Abstract

The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (~175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.

Keywords: Neisseria sp.;Siphoviridae;lytic phage A2;human dental plaques;contigs;phylogenetic relationships

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Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque

J. Microbiol. 2014;52(7):609-618. Published online May 30, 2014

DOI: https://doi.org/10.1007/s12275-014-3380-1

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