Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells

Qiang Fu; Huijun Shi; Yan Ren; Fei Guo; Wei Ni; Jun Qiao; Pengyan Wang; Hui Zhang; Chuangfu Chen

doi:10.1007/s12275-014-3479-4

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Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells: Qiang Fu ¹, Huijun Shi ¹, Yan Ren ², Fei Guo ², Wei Ni ³, Jun Qiao ¹, Pengyan Wang ¹, Hui Zhang ¹, Chuangfu Chen ¹; Journal of Microbiology 2014;52(7):619-625.
DOI: https://doi.org/10.1007/s12275-014-3479-4
Published online: June 28, 2014

¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, P.R. China, ²College of Medicine, Shihezi University, Shihezi 832003, Xinjiang, P.R. China, ³College of Life Science, Shihezi University, Shihezi 832003, Xinjiang, P.R. China¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, P.R. China, ²College of Medicine, Shihezi University, Shihezi 832003, Xinjiang, P.R. China, ³College of Life Science, Shihezi University, Shihezi 832003, Xinjiang, P.R. China

Corresponding author: Chuangfu Chen , Tel: +86-993-2058002,

Received: 13 September 2013 • Revised: 20 February 2014 • Accepted: 5 April 2014

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Abstract

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

Keywords: bovine viral diarrhea virus NADL;autophagy;Madin-Darby Bovine Kidney (MDBK) cells;autophagosome;GFP-LC3

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Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells

J. Microbiol. 2014;52(7):619-625. Published online June 28, 2014

DOI: https://doi.org/10.1007/s12275-014-3479-4

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