Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi

Yue Wang; Kuan-Yeh Huang; Yanan Huo

doi:10.1007/s12275-014-3204-3

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Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi: Yue Wang ¹, Kuan-Yeh Huang ^2,3, Yanan Huo ⁴; Journal of Microbiology 2014;52(1):71-76.
DOI: https://doi.org/10.1007/s12275-014-3204-3
Published online: January 4, 2014

¹Department of Gynecology and Obstetrics, Women’s Hospital School of Medicine Zhejiang University, Hangzhou, P. R. China, ²Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan, ³Molecualr Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan, ⁴Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang University, Hangzhou, P. R. China¹Department of Gynecology and Obstetrics, Women’s Hospital School of Medicine Zhejiang University, Hangzhou, P. R. China, ²Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan, ³Molecualr Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan, ⁴Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang University, Hangzhou, P. R. China

Received: 8 April 2013 • Revised: 30 July 2013 • Accepted: 5 August 2013

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Abstract

The genus Salmonella contains more than 2500 serovars. While most cause the self-limiting gastroenteritis, a few serovars can elicit typhoid fever, a severe systemic infection. S. enterica subsp. enterica serovar Typhimurium and S. Typhi are the representatives of the gastroenteritis and typhoid fever types of Salmonella. In this study, we adopted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) technology to quantitatively compare the proteomes of the two serovars. We found several proteins with serovar- specific expression, which could be developed as new biomarkers for clinical serotype diagnosis. We found that flagella and chemotaxis genes were down-regulated in S. Typhi in comparison with S. Typhimurium. We attributed this observation to the fact that the smooth cellular structure of S. Typhi may better fit its systemic lifestyle. Instead of known virulence factors that were located within Salmonella Pathogenecity Islands, a number of core genes, which were involved in metabolism and transport of carbohydrates and amino acids, showed differential expression between the two serovars. Further studies on the roles of these differentially- expressed genes in the pathogenesis should be undertaken.

Keywords: SILAC;proteome;Salmonella;typhoid;biomarker

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Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi

J. Microbiol. 2014;52(1):71-76. Published online January 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3204-3

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