Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression

Mi-Sun Kang; Jin-Hee Kim; Boo-Ahn Shin; Hyun-Chul Lee; Youn-Shin Kim; Hae-Soon Lim; Jong-Suk Oh

doi:10.1007/s12275-013-3015-y

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Research Support, Non-U.S. Gov't
Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression: Mi-Sun Kang ¹, Jin-Hee Kim ¹, Boo-Ahn Shin ¹, Hyun-Chul Lee ¹, Youn-Shin Kim ², Hae-Soon Lim ³, Jong-Suk Oh ¹; Journal of Microbiology 2013;51(6):844-849.
DOI: https://doi.org/10.1007/s12275-013-3015-y
Published online: December 19, 2013

¹Department of Microbiology, School of Medicine, Chonnam National University, Gwangju 501-746, Republic of Korea, ²Department of Forensic Medicine, School of Medicine, Chosun University, Gwangju 501-759, Republic of Korea, ³Department of Dental Education, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea¹Department of Microbiology, School of Medicine, Chonnam National University, Gwangju 501-746, Republic of Korea, ²Department of Forensic Medicine, School of Medicine, Chosun University, Gwangju 501-759, Republic of Korea, ³Department of Dental Education, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea

Corresponding author: Jong-Suk Oh , Tel: +82-62-220-4134,

Received: 8 January 2013 • Accepted: 10 June 2013

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Abstract

Chlorophyllin (CHL), a chlorophyll-derivative, exhibits several beneficial properties, including antibacterial, antioxidant, and anticancer activities. However, its antibacterial and anti-inflammatory activities against Propionibacterium acnes have not been described. The antibacterial activity of this compound was evaluated in vitro using the broth microdilution
method
. CHL had an inhibitory effect on the growth of P. acnes (MIC = 100 μM). In a real-time reverse transcriptionpolymerase chain reaction and an enzyme-linked immunosorbent assay, CHL significantly decreased interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) production in a dose-dependent manner, decreasing both mRNA and protein levels for these chemokines in THP-1 cells indicating the anti-inflammatory effects of it. To investigate the molecular mechanisms underlying the anti-inflammatory properties of CHL in THP-1 cells stimulated by P. acnes, we used western blotting to analyze the effect of CHL on activation of the nuclear factor (NF)-κB. CHL inhibited P. acnes-induced IL-8 and MCP-1 production via blockade of NF-κB activation in THP-1 cells. Therefore, based on these results, we suggest that CHL is a useful agent to control the growth of P. acnes involved in acne inflammation and prevent acne.

Keywords: chlorophyllin;interleukin-8;monocyte chemotactic protein 1;Propionibacterium acnes;THP-1 cells

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Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression

J. Microbiol. 2013;51(6):844-849. Published online December 19, 2013

DOI: https://doi.org/10.1007/s12275-013-3015-y

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