Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells

Jia-He Wang; Ke Zhang; Nan Wang; Xiao-Min Qiu; Yi-Bing Wang; Ping He

doi:10.1007/s12275-013-3040-x

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Research Support, Non-U.S. Gov't
Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells: Jia-He Wang ¹, Ke Zhang ², Nan Wang ¹, Xiao-Min Qiu ¹, Yi-Bing Wang ³, Ping He ¹; Journal of Microbiology 2013;51(5):644-650.
DOI: https://doi.org/10.1007/s12275-013-3040-x
Published online: June 25, 2013

¹Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, 110004, P. R. China, ²2Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110004, P. R. China , ³Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, P. R. China¹Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, 110004, P. R. China, ²2Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110004, P. R. China , ³Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, P. R. China

Corresponding author: Jia-He Wang , Tel: .,

Received: 18 January 2013 • Accepted: 15 April 2013

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Abstract

The invasion of Staphylococcus aureus into alveolar epithelial cells is regarded as the key step for S. aureus lung infection. However, the mechanism of internalization of S. aureus by alveolar epithelial cells is not clear, and was the aim of this investigation Human lung adenocarcinomic epithelial cells and A549 cells were used. Human β1 integrin and rat β1 integrin were detected by real-time reverse transcription (RT)-PCR. The expressions of β1 integrin, Akt and p-Akt were detected by Western blot analysis. To further investigate the role of β1 integrin in S. aureus internalization by alveolar epithelial cells, we next performed siRNA-mediated knockdown of β1 integrin expression. In this study, we found that S. aureus invades human alveolar epithelial cells and rat primary alveolar epithelial cells. The β1 integrin ligand competitive inhibitor, GRGDS-peptide, blocked the internalization of S. aureus by A549 cells. Knockdown of β1 integrin also inhibited the internalization of S. aureus. In addition, the PI3K/Akt signaling pathway in alveolar epithelial cells was activated by the infection with S. aureus. Furthermore, Akt phosphorylation was abolished by transient transfection with β1 integrin siRNA in A549 cells challenged with S. aureus. Our results suggest that the phosphatidylinositol 3-kinase/Akt signaling pathway plays an important role in β1 integrin-mediated internalization of S. aureus by alveolar epithelial cells.

Keywords: Staphylococcus aureus;phosphatidylinositol 3-kinase;β1 integrin;alveolar epithelial cells;internalization

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Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells

J. Microbiol. 2013;51(5):644-650. Published online June 25, 2013

DOI: https://doi.org/10.1007/s12275-013-3040-x

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